Inbred corn plant 85DGD1 and seeds thereof

ABSTRACT

According to the invention, there is provided inbred corn plants, separately designated GMLEA, 6F905, 91CSV-1, 91DFA-5, WDAQ2, WDDQ1, 85DGD1, PHEI4, 01ASB1, 01IBH2, NL054B and FBMU. This invention thus relates to the plants, seeds and tissue cultures of the fore-mentioned inbred corn plants and to methods for producing a corn plant produced by crossing one of the inbred plants with itself or with another corn plant, such as another inbred. This invention further relates to corn seeds and plants produced by crossing any of the inbred plants GMLEA, 6F905, 91CSV-1, 91DFA-5, WDAQ2, WDDQ1, 85DGD1, PHEI4, 01ASB1, 01IBH2, NL054B or FBMU with another corn plant, such as another inbred, and to crosses with related species. This invention further relates to the inbred and hybrid genetic complements of the inbred corn plants GMLEA, 6F905, 91CSV-1, 91DFA-5, WDAQ2, WDDQ1, 85DGD1, PHEI4, 01ASB1, 01IBH2, NL054B and FBMU, and also to the RFLP and genetic isozyme typing profiles of such inbred corn plants.

This application is a continuation application of U.S. patentapplication Ser. No. 09/693,329, filed Oct. 19, 2000, now abandoned,which application is a continuation application of Ser. No. 08/702,920,filed Aug. 26, 1996, now issued as U.S. Pat. No. 6,372,969, whichapplication is a continuation-in-part application of U.S. patentapplication Ser. No. 08/384,266, filed Feb. 3, 1995, now abandoned, thedisclosure of which is incorporated herein by reference.

BACKGROUND OF THE INVENTION

I. Technical Field of the Invention

The present invention relates to the field of corn breeding. Inparticular, the invention relates to inbred corn seed and plantsdesignated GMLEA, 6F905, 91CSV-1, 91DFA-5, WDAQ2, WDDQ1, 85DGD1, PHEI4,01ASB1, 01IBH2, NL054B and FBMU, and derivatives and tissue cultures ofsuch inbred plants.

II. Description of the Background Art

The goal of field crop breeding is to combine various desirable traitsin a single variety/hybrid. Such desirable traits include greater yield,better stalks, better roots, resistance to insecticides, pests, anddisease, tolerance to heat and drought, reduced time to crop maturity,better agronomic quality, and uniformity in germination times, standestablishment, growth rate, maturity, and fruit size.

Breeding techniques take advantage of a plant's method of pollination.There are two general methods of pollination: a plant self-pollinates ifpollen from one flower is transferred to the same or another flower ofthe same plant. A plant cross-pollinates if pollen comes to it from aflower on a different plant.

Corn plants (Zea mays L.) can be bred by both self-pollination andcross-pollination. Both types of pollination involve the corn plant'sflowers. Corn has separate male and female flowers on the same plant,located on the tassel and the ear, respectively. Natural pollinationoccurs in corn when wind blows pollen from the tassels to the silks thatprotrude from the tops of the ears.

Plants that have been self-pollinated and selected for type over manygenerations become homozygous at almost all gene loci and produce auniform population of true breeding progeny, a homozygous plant. A crossbetween two such homozygous plants produce a uniform population ofhybrid plants that are heterozygous for many gene loci. Conversely, across of two plants each heterozygous at a number of gene loci producesa population of hybrid plants that differ genetically and are notuniform. The resulting non-uniformity make performance unpredictable.

The development of uniform corn plant hybrids requires the developmentof homozygous inbred plants, the crossing of these inbred plants, andthe evaluation of the crosses. Pedigree breeding and recurrent selectionbreeding methods are used to develop inbred plants from breedingpopulations. Those breeding methods combine the genetic backgrounds fromtwo or more inbred plants or various other broad-based sources intobreeding pools from which new inbred plants are developed by selfing andselection of desired phenotypes. The new inbreds are crossed with otherinbred plants and the hybrids from these crosses are evaluated todetermine which of those have commercial potential.

The pedigree breeding method for single-gene traits involves crossingtwo genotypes. Each genotype can have one or more desirablecharacteristics lacking in the other; or, each genotype can complementthe other. If the two original parental genotypes do not provide all ofthe desired characteristics, other genotypes can be included in thebreeding population. Superior plants that are the products of thesecrosses are selfed and selected in successive generations. Eachsucceeding generation becomes more homogeneous as a result ofself-pollination and selection. Typically, this method of breedinginvolves five or more generations of selfing and selection: S₁→S₂;S₂→S₃; S₃→S₄; S₄→S₅, etc. After at least five generations, the inbredplant is considered genetically pure.

Backcrossing can also be used to improve an inbred plant. Backcrossingtransfers a specific desirable trait from one inbred or source to aninbred that lacks that trait. This can be accomplished for example byfirst crossing a superior inbred (A) (recurrent parent) to a donorinbred (non-recurrent parent), which carries the appropriate gene(s) forthe trait in question. The progeny of this cross are then mated back tothe superior recurrent parent (A) followed by selection in the resultantprogeny for the desired trait to be transferred from the non-recurrentparent. After five or more backcross generations with selection for thedesired trait, the progeny are heterozygous for loci controlling thecharacteristic being transferred, but are like the superior parent formost or almost all other genes. The last backcross generation would beselfed to give pure breeding progeny for the gene(s) being transferred.

A single cross hybrid corn variety is the cross of two inbred plants,each of which has a genotype which complements the genotype of theother. The hybrid progeny of the first generation is designated F₁.Preferred F₁ hybrids are more vigorous than their inbred parents. Thishybrid vigor, or heterosis, is manifested in many polygenic traits,including markedly improved higher yields, better stalks, better roots,better uniformity and better insect and disease resistance. In thedevelopment of hybrids only the F₁ hybrid plants are sought. An F₁single cross hybrid is produced when two inbred plants are crossed. Adouble cross hybrid is produced from four inbred plants crossed in pairs(A×B and C×D) and then the two F₁ hybrids are crossed again (A×B)×(C×D).

The development of a hybrid corn variety involves three steps: (1) theselection of plants from various germplasm pools; (2) the selfing of theselected plants for several generations to produce a series of inbredplants, which, although different from each other, each breed true andare highly uniform; and (3) crossing the selected inbred plants withunrelated inbred plants to produce the hybrid progeny (F₁). During theinbreeding process in corn, the vigor of the plants decreases. Vigor isrestored when two unrelated inbred plants are crossed to produce thehybrid progeny (F₁). An important consequence of the homozygosity andhomogeneity of the inbred plants is that the hybrid between any twoinbreds is always the same. Once the inbreds that give a superior hybridhave been identified, hybrid seed can be reproduced indefinitely as longas the homogeneity of the inbred parents is maintained. Conversely, muchof the hybrid vigor exhibited by F₁ hybrids is lost in the nextgeneration (F₂). Consequently, seed from hybrid varieties is not usedfor planting stock. It is not generally beneficial for farmers to saveseed of F₁ hybrids. Rather, farmers purchase F₁ hybrid seed for plantingevery year.

North American farmers plant over 70 million acres of corn at thepresent time and there are extensive national and internationalcommercial corn breeding programs. A continuing goal of these cornbreeding programs is to develop high-yielding corn hybrids that arebased on stable inbred plants that maximize the amount of grain producedand minimize susceptibility to environmental stresses. To accomplishthis goal, the corn breeder must select and develop superior inbredparental plants for producing hybrids.

SUMMARY OF THE INVENTION

In one aspect, the present invention provides corn plants designated,separately, GMLEA, 6F905, 91CSV-1, 91DFA-5, WDAQ2, WDDQ1, 85DGD1, PHEI4,01ASB1, 01IBH2, NL054B and FBMU. Also provided are corn plants havingthe functional and morphological characteristics of corn plants GMLEA,6F905, 91CSV-1, 91DFA-5, WDAQ2, WDDQ1, 85DGD1, PHEI4, 01ASB1, 01IBH2,NL054B or FBMU, as exemplified by corn plants having all thephysiological and morphological characteristics of corn plants GMLEA,6F905, 91CSV-1, 91DFA-5, WDAQ2, WDDQ1, 85DGD1, PHEI4, 01ASB1, 01IBH2,NL054B or FBMU.

The inbred corn plants of the invention may further comprise, or have, acytoplasmic factor that is capable of conferring male sterility. Partsof the corn plants of the present invention are also provided, such as,e.g., pollen obtained from an inbred plant and an ovule of the inbredplant.

The invention also concerns seed of the corn plant 85DGD1 which has beendeposited with the ATCC. The invention thus provides inbred corn seeddesignated 85DGD1 and having ATCC Accession No. PTA-6160.

The inbred corn seed of the invention may be provided as essentiallyhomogeneous populations of inbred corn seed designated GMLEA, 6F905,91CSV-1, 91DFA-5, WDAQ2, WDDQ1, 85DGD1, PHEI4, 01ASB1, 01IBH2, NL054B orFBMU. Essentially homogeneous populations of inbred seed are those thatconsist essentially of the particular inbred seed and are generallypurified free from substantial numbers of other seed, so that the inbredseed forms between about 90% and about 100% of the total seed, andpreferably, between about 95% and about 100% of the total seed. Mostpreferably, an essentially homogeneous population of inbred corn seedwill contain between about 98.5%, 99%, 99.5% and about 100% of inbredseed, as measured by seed grow outs.

In any event, even if a population of inbred corn seed was found, forsome reason, to contain about 50%, or even about 20% or 15% of inbredseed, this would still be distinguished from the small fraction ofinbred seed that may be found within a population of hybrid seed, e.g.,within a bag of hybrid seed. In such a bag of hybrid seed offered forsale, the Governmental regulations require that the hybrid seed be atleast about 95% of the total seed. In the practice of the presentinventors, the hybrid seed generally forms at least about 97% of thetotal seed. In the most preferred practice of the inventors, the femaleinbred seed that may be found within a bag of hybrid seed will be about1% of the total seed, or less, and the male inbred seed that may befound within a bag of hybrid seed will be negligible, i.e., will be onthe order of about a maximum of 1 per 100,000, and usually less thanthis value.

The populations of inbred corn seed of the invention are furtherparticularly defined as being essentially free from hybrid seed. Theinbred seed populations may be separately grown to provide essentiallyhomogeneous populations of inbred corn plants designated GMLEA, 6F905,91CSV-1, 91DFA-5, WDAQ2, WDDQ1, 85DGD1, PHEI4, 01ASB1, 01IBH2, NL054B orFBMU.

In another aspect, the present invention provides a tissue culture ofregenerable cells of inbred corn plant GMLEA, 6F905, 91CSV-1, 91DFA-5,WDAQ2, WDDQ1, 85DGD1, PHEI4, 01ASB1, 01IBH2, NL054B or FBMU. The tissueculture will preferably be capable of regenerating plants having thephysiological and morphological characteristics of one of the foregoinginbred corn plants, and of regenerating plants having substantially thesame genotype as one of the foregoing inbred corn plants. Preferably,the regenerable cells in such tissue cultures will be embryos,protoplasts, meristematic cells, pollen, leaves, anthers, roots, roottips, silk, flowers, kernels, ears, cobs, husks or stalks. Stillfurther, the present invention provides corn plants regenerated from oneof the tissue cultures of the invention.

In another aspect, the present invention provides for single geneconverted plants of GMLEA, 6F905, 91CSV-1, 91DFA-5, WDAQ2, WDDQ1,85DGD1, PHEI4, 01ASB1, 01IBH2, NL054B or FBMU. The single transferredgene may preferably be a dominant or recessive allele. Preferably, thesingle transferred gene will confer such traits as male sterility,herbicide resistance, insect resistance, resistance for bacterial,fungal, or viral disease, male fertility, enhanced nutritional quality,and industrial usage.

In yet another aspect, the present invention provides processes forpreparing corn seed or plants, which processes generally comprisecrossing a first parent corn plant with a second parent corn plant,wherein at least one of the first or second parent corn plants is theinbred corn plant designated GMLEA, 6F905, 91CSV-1, 91DFA-5, WDAQ2,WDDQ1, 85DGD1, PHEI4, 01ASB1, 01IBH2, NL054B or FBMU. These processesmay be further exemplified as processes for preparing hybrid corn seedor plants, wherein a first inbred corn plant is crossed with a second,distinct inbred corn plant to provide a hybrid that has, as one of itsparents, the inbred corn plant GMLEA, 6F905, 91CSV-1, 91DFA-5, WDAQ2,WDDQ1, 85DGD1, PHEI4, 01ASB1, 01IBH2, NL054B or FBMU.

In a preferred embodiment, crossing comprises planting, in pollinatingproximity, seeds of the first and second parent corn plant, andpreferably, seeds of a first inbred corn plant and a second, distinctinbred corn plant; cultivating or growing the seeds of said first andsecond parent corn plants into plants that bear flowers; emasculatingthe male flowers of the first or second parent corn plant, i.e.,treating the flowers so as to prevent pollen production, in order toproduce an emasculated parent corn plant; allowing naturalcross-pollination to occur between the first and second parent cornplants; and harvesting the seeds from the emasculated parent corn plant.Where desired, the harvested seed is grown to produce a corn plant orhybrid corn plant.

The present invention also provides corn seed and plants produced by aprocess that comprises crossing a first parent corn plant with a secondparent corn plant, wherein at least one of the first or second parentcorn plants is the inbred corn plant designated GMLEA, 6F905, 91CSV-1,91DFA-5, WDAQ2, WDDQ1, 85DGD1, PHEI4, 01ASB1, 01IBH2, NL054B or FBMU. Inone embodiment, corn plants produced by the process are first generation(F₁) hybrid corn plants produced by crossing an inbred in accordancewith the invention with another, distinct inbred. The present inventionfurther contemplates seed of an F₁ hybrid corn plant.

In certain exemplary embodiments, the invention provides the followingF₁ hybrid corn plants and seed thereof:

-   -   hybrid corn plant designated DK706, having GMLEA and 6F905, both        inbreds of the invention, as parents;    -   hybrid corn plant designated DK446, having 91CSV-1 and 01ASB1,        both inbreds of the invention, as parents;    -   hybrid corn plant designated DK604, having WDAQ2 and 01IBH2,        both inbreds of the invention, as parents;    -   hybrid corn plant designated DK527, having 01IBH2 and FBMU, both        inbreds of the invention, as parents;    -   hybrid corn plant designated DK474, having 91DFA-5 as one inbred        parent;    -   hybrid corn plant designated DK642, having WDDQ1 as one inbred        parent;    -   hybrid corn plant designated DK560, having 85DGD1 as one inbred        parent;    -   hybrid corn plant designated DK566, having PHEI4 as one inbred        parent;    -   hybrid corn plant designated DK442, also having 01IBH2 as one        inbred parent; and    -   hybrid corn plant designated DK626, having NL054B as one inbred        parent.

It should be noted here that although the present invention provides 12inbreds, the details concerning only 10 exemplary hybrids are set forthherein. This results from the crossing of certain of the claimed inbredswith other inbreds of this invention. Four hybrids are disclosed hereinthat have both parents from the presently claimed inbreds, namely DK706,DK446, DK604, and DK527. Also, the inbred 01IBH2 is employed to generatethree different, particularly successful hybrids. Accounting for thesevarious combinations results in the ten hybrids disclosed herein thathave commercially advantageous features. Table 30 is provided herein forinstant cross-reference of the disclosed inbreds and exemplary hybrids.

In yet a further aspect, the invention provides inbred geneticcomplements of corn plants designated, separately, GMLEA, 6F905,91CSV-1, 91DFA-5, WDAQ2, WDDQ1, 85DGD1, PHEI4, 01ASB1, 01IBH2, NL054Band FBMU. The phrase “genetic complement” is used to refer to theaggregate of nucleotide sequences, the expression of which sequencesdefines the phenotype of, in the present case, a corn plant, or a cellor tissue of that plant. An inbred genetic complement thus representsthe genetic make up of an inbred cell, tissue or plant, and a hybridgenetic complement represents the genetic make up of a hybrid cell,tissue or plant. The invention thus provides corn plant cells that havea genetic complement in accordance with the inbred corn plant cellsdisclosed herein, and plants, seeds and diploid plants containing suchcells.

Plant genetic complements may be assessed by genetic marker profiles,and by the expression of phenotypic traits that are characteristic ofthe expression of the genetic complement, e.g., isozyme typing profiles.Thus such corn plant cells may be defined as having an RFLP geneticmarker profile in accordance with the profile shown in Table 52, Table53, Table 54, Table 55, Table 56, Table 57, Table 58, Table 59, Table60, Table 61, Table 62 or Table 63; or a genetic isozyme typing profilein accordance with the profile shown in Table 64, Table 65, Table 66,Table 67, Table 68, Table 69, Table 70, Table 71, Table 72, Table 73,Table 74 or Table 75; or having both an RFLP genetic marker profile anda genetic isozyme typing profile in accordance with the profiles shownin Tables 52 and 64; Tables 53 and 65; Tables 54 and 66; Tables 55 and67; Tables 56 and 68; Tables 57 and 69; Tables 58 and 70; Tables 59 and71; Tables 60 and 72; Tables 61 and 73; Tables 62 and 74; or as shown inboth Tables 63 and 75.

In another aspect, the present invention provides hybrid geneticcomplements, as represented by corn plant cells, tissues, plants andseeds, formed by the combination of a haploid genetic complement of aninbred corn plant of the invention with a haploid genetic complement ofa second corn plant, preferably, another, distinct inbred corn plant. Inanother aspect, the present invention provides a corn plant regeneratedfrom a tissue culture that comprises a hybrid genetic complement of thisinvention.

DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS

I. Definitions

-   Barren Plants:    -   Plants that are barren, i.e. lack an ear with grain, or have an        ear with only a few scattered kernels.-   cg:    -   Colletotrichum graminicola rating. Rating times 10 is        approximately equal to percent total plant infection.-   CLN:    -   Corn lethal necrosis (combination of Maize Chlorotic Mottle        Virus and Maize Dwarf Mosaic virus) rating: numerical ratings        are based on a severity scale where 1=most resistant to        9=susceptible.-   Cn:    -   Corynebacterium nebraskense rating. Rating times 10 is        approximately equal to percent total plant infection.-   Cz:    -   Cercospora zeae-maydis rating. Rating times 10 is approximately        equal to percent total plant infection.-   Dgg:    -   Diatraea grandiosella girdling rating (values are percent plants        girdled and stalk lodged).-   Dropped Ears:    -   Ears that have fallen from the plant to the ground.-   Dsp:    -   Diabrotica species root ratings (1=least affected to 9=severe        pruning).-   Ear-Attitude:    -   The attitude or position of the ear at harvest scored as        upright, horizontal, or pendant.-   Ear-Cob Color:    -   The color of the cob, scored as white, pink, red, or brown.-   Ear-Cob Diameter:    -   The average diameter of the cob measured at the midpoint.-   Ear-Cob Strength:    -   A measure of mechanical strength of the cobs to breakage, scored        as strong or weak.-   Ear-Diameter:    -   The average diameter of the ear at its midpoint.-   Ear-Dry Husk Color:    -   The color of the husks at harvest scored as buff, red, or        purple.-   Ear-Fresh Husk Color:    -   The color of the husks 1 to 2 weeks after pollination scored as        green, red, or purple.-   Ear-Husk Bract:    -   The length of an average husk leaf scored as short, medium, or        long.-   Ear-Husk Cover:    -   The average distance from the tip of the ear to the tip of the        husks. Minimum value no less than zero.-   Ear-Husk opening:    -   An evaluation of husk tightness at harvest scored as tight,        intermediate, or open.-   Ear-Length:    -   The average length of the ear.-   Ear-Number Per Stalk:    -   The average number of ears per plant.-   Ear-Shank Internodes:    -   The average number of internodes on the ear shank.-   Ear-Shank Length:    -   The average length of the ear shank.-   Ear-Shelling Percent:    -   The average of the shelled grain weight divided by the sum of        the shelled grain weight and cob weight for a single ear.-   Ear-Silk Color:    -   The color of the silk observed 2 to 3 days after silk emergence        scored as green-yellow, yellow, pink, red, or purple.-   Ear-Taper (Shape):    -   The taper or shape of the ear scored as conical, semi-conical,        or cylindrical.-   Ear-Weight:    -   The average weight of an ear.-   Early Stand:    -   The percent of plants that emerge from the ground as determined        in the early spring.-   ER:    -   Ear rot rating (values approximate percent ear rotted).-   Final Stand Count:    -   The number of plants just prior to harvest.-   GDUs to Shed:    -   The number of growing degree units (GDUs) or heat units required        for an inbred line or hybrid to have approximately 50 percent of        the plants shedding pollen as measured from time of planting.        Growing degree units are calculated by the Barger Method, where        the heat units for a 24-hour period are calculated as        GDUs=[Maximum daily temperature+Minimum daily        temperature)/2]−50. The highest maximum daily temperature used        is 86 degrees Fahrenheit and the lowest minimum temperature used        is 50 degrees Fahrenheit. GDUs to shed is then determined by        summing the individual daily values from planting date to the        date of 50 percent pollen shed.-   GDUs to silk:    -   The number of growing degree units for an inbred line or hybrid        to have approximately 50 percent of the plants with silk        emergence as measured from time of planting. Growing degree        units are calculated by the same methodology as indicated in the        GDUs to shed definition.-   Hc2:    -   Helminthosporium carbonum race 2 rating. Rating times 10 is        approximately equal to percent total plant infection.-   Hc3:    -   Helminthosporium carbonum race 3 rating. Rating times 10 is        approximately equal to percent total plant infection.-   Hm:    -   Helminthosporium Maydis race 0 rating. Rating times 10 is        approximately equal to percent total plant infection.-   Ht1:    -   Helminthosporium turcicum race 1 rating. Rating times 10 is        approximately equal to percent total plant infection.-   Ht2:    -   Helminthosporium turcicum race 2 rating. Rating times 10 is        approximately equal to percent total plant infection.-   HtG:    -   +=Presence of Ht chlorotic-lesion type resistance. Rating times        10 is approximately equal to percent total plant infection.    -   −=Absence of a Ht chlorotic-lesion type resistance. Rating times        10 is approximately equal to percent total plant infection.    -   +/−=Segregation of a Ht chlorotic-lesion type resistance. Rating        times 10 is approximately equal to percent total plant        infection.-   Kernel-Aleurone Color:    -   The color of the aleurone scored as white, pink, tan, brown,        bronze, red, purple, pale purple, colorless, or variegated.-   Kernel-Cap Color:    -   The color of the kernel cap observed at dry stage, scored as        white, lemon-yellow, yellow or orange.-   Kernel-Endosperm Color:    -   The color of the endosperm scored as white, pale yellow, or        yellow.-   Kernel-Endosperm Type:    -   The type of endosperm scored as normal, waxy, or opaque.-   Kernel-Grade:    -   The percent of kernels that are classified as rounds.-   Kernel-Length:    -   The average distance from the cap of the kernel to the pedicel.-   Kernel-Number Per Row:    -   The average number of kernels in a single row.-   Kernel-Pericarp Color:    -   The color of the pericarp scored as colorless, red-white crown,        tan, bronze, brown, light red, cherry red, or variegated.-   Kernel-Row Direction:    -   The direction of the kernel rows on the ear scored as straight,        slightly curved, spiral, or indistinct (scattered).-   Kernel-Row Number:    -   The average number of rows of kernels on a single ear.-   Kernel-Side Color:    -   The color of the kernel side observed at the dry stage, scored        as white, pale yellow, yellow, orange, red, or brown.-   Kernel-Thickness:    -   The distance across the narrow side of the kernel.-   Kernel-Type:    -   The type of kernel scored as dent, flint, or intermediate.-   Kernel-Weight:    -   The average weight of a predetermined number of kernels.-   Kernel-Width:    -   The distance across the flat side of the kernel.-   Kz:    -   Kabatiella zeae rating. Rating times 10 is approximately equal        to percent total plant infection.-   Leaf-Angle:    -   Angle of the upper leaves to the stalk scored as upright (0 to        30 degrees), intermediate (30 to 60 degrees), or lax (60 to 90        degrees).-   Leaf-Color:    -   The color of the leaves 1 to 2 weeks after pollination scored as        light green, medium green, dark green, or very dark green.-   Leaf-Length:    -   The average length of the primary ear leaf.-   Leaf-Longitudinal Creases:    -   A rating of the number of longitudinal creases on the leaf        surface 1 to 2 weeks after pollination. Creases are scored as        absent, few, or many.-   Leaf-Marginal Waves:    -   A rating of the waviness of the leaf margin 1 to 2 weeks after        pollination. Rated as none, few, or many.-   Leaf-Number:    -   The average number of leaves of a mature plant. Counting begins        with the cotyledonary leaf and ends with the flag leaf.-   Leaf-Sheath Anthocyanin:    -   A rating of the level of anthocyanin in the leaf sheath 1 to 2        weeks after pollination, scored as absent, basal-weak,        basal-strong, weak or strong.-   Leaf-Sheath Pubescence:    -   A rating of the pubescence of the leaf sheath. Ratings are taken        1 to 2 weeks after pollination and scored as light, medium, or        heavy.-   Leaf-Width:    -   The average width of the primary ear leaf measured at its widest        point.-   LSS:    -   Late season standability (values times 10 approximate percent        plants lodged in disease evaluation plots).-   Moisture:    -   The moisture of the grain at harvest.-   On1:    -   Ostrinia nubilalis 1st brood rating (1=resistant to        9=susceptible).-   On2:    -   Ostrinia nubilalis 2nd brood rating (1=resistant to        9=susceptible).-   Relative Maturity:    -   A maturity rating based on regression analysis. The regression        analysis is developed by utilizing check hybrids and their        previously established day rating versus actual harvest        moistures. Harvest moisture on the hybrid in question is        determined and that moisture value is inserted into the        regression equation to yield a relative maturity.-   Root Lodging:    -   Root lodging is the percentage of plants that root lodge. A        plant is counted as root lodged if a portion of the plant leans        from the vertical axis by approximately 30 degrees or more.-   Seedling Color:    -   Color of leaves at the 6 to 8 leaf stage.-   Seedling Height:    -   Plant height at the 6 to 8 leaf stage.-   Seedling Vigor:    -   A visual rating of the amount of vegetative growth on a 1 to 9        scale, where 9 equals best. The score is taken when the average        entry in a trial is at the fifth leaf stage.-   Selection Index:    -   The selection index gives a single measure of hybrid's worth        based on information from multiple traits. One of the traits        that is almost always included is yield. Traits may be weighted        according to the level of importance assigned to them.-   Sr:    -   Sphacelotheca reiliana rating is actual percent infection.-   Stalk-Anthocyanin:    -   A rating of the amount of anthocyanin pigmentation in the stalk.        The stalk is rated 1 to 2 weeks after pollination as absent,        basal-weak, basal-strong, weak, or strong.-   Stalk-Brace Root Color:    -   The color of the brace roots observed 1 to 2 weeks after        pollination as green, red, or purple.-   Stalk-Diameter:    -   The average diameter of the lowest visible internode of the        stalk.-   Stalk-Ear Height:    -   The average height of the ear measured from the ground to the        point of attachment of the ear shank of the top developed ear to        the stalk.-   Stalk-Internode Direction:    -   The direction of the stalk internode observed after pollination        as straight or zigzag.-   Stalk-Internode Length:    -   The average length of the internode above the primary ear.-   Stalk Lodging:    -   The percentage of plants that did stalk lodge. Plants are        counted as stalk lodged if the plant is broken over or off below        the ear.-   Stalk-Nodes With Brace Roots:    -   The average number of nodes having brace roots per plant.-   Stalk-Plant Height:    -   The average height of the plant as measured from the soil to the        tip of the tassel.-   Stalk-Tillers:    -   The percent of plants that have tillers. A tiller is defined as        a secondary shoot that has developed as a tassel capable of        shedding pollen.-   Staygreen:    -   Staygreen is a measure of general plant health near the time of        black layer formation (physiological maturity). It is usually        recorded at the time the ear husks of most entries within a        trial have turned a mature color. Scoring is on a 1 to 9 basis        where 9 equals best.-   STR:    -   Stalk rot rating (values represent severity rating of 1=25        percent of inoculated internode rotted to 9=entire stalk rotted        and collapsed).-   SVC:    -   Southeastern Virus Complex combination of Maize Chlorotic Dwarf        Virus and Maize Dwarf Mosaic Virus) rating; numerical ratings        are based on a severity scale where 1=most resistant to        9=susceptible (1988 reactions are largely Maize Dwarf Mosaic        Virus reactions).-   Tassel-Anther Color:    -   The color of the anthers at 50 percent pollen shed scored as        green-yellow, yellow, pink, red, or purple.-   Tassel-Attitude:    -   The attitude of the tassel after pollination scored as open or        compact.-   Tassel-Branch Angle:    -   The angle of an average tassel branch to the main stem of the        tassel scored as upright (less than 30 degrees), intermediate        (30 to 45 degrees), or lax (greater than 45 degrees).-   Tassel-Branch Number:    -   The average number of primary tassel branches.-   Tassel-Glume Band:    -   The closed anthocyanin band at the base of the glume scored as        present or absent.-   Tassel-Glume Color:    -   The color of the glumes at 50 percent shed scored as green, red,        or purple.-   Tassel-Length:    -   The length of the tassel measured from the base of the bottom        tassel branch to the tassel tip.-   Tassel-Peduncle Length:    -   The average length of the tassel peduncle, measured from the        base of the flag leaf to the base of the bottom tassel branch.-   Tassel-Pollen Shed:    -   A visual rating of pollen shed determined by tapping the tassel        and observing the pollen flow of approximately five plants per        entry. Rated on a 1 to 9 scale where 9=sterile, 1=most pollen.-   Tassel-Spike Length:    -   The length of the spike measured from the base of the top tassel        branch to the tassel tip.-   Test Weight:    -   The measure of the weight of the grain in pounds for a given        volume (bushel) adjusted to 15.5 percent moisture.-   Yield:    -   Yield of grain at harvest adjusted to 15.5 percent moisture.        II. Other Definitions

Allele is any of one or more alternative forms of a gene, all of whichalleles relate to one trait or characteristic. In a diploid cell ororganism, the two alleles of a given gene occupy corresponding loci on apair of homologous chromosomes.

Backcrossing is a process in which a breeder crosses a first generationhybrid (F₁) with one of the parental genotypes.

Chromatography is a technique wherein a mixture of dissolved substancesare bound to a solid support followed by passing a column of fluidacross the solid support and varying the composition of the fluid. Thecomponents of the mixture are separated by selective elution.

Crossing refers to the mating of two parent plants.

Cross-pollination refers to fertilization by the union of two gametesfrom different plants.

Diploid refers to a cell or organism having two sets of chromosomes.

Electrophoresis is a process by which particles suspended in a fluid aremoved under the action of an electrical field, and thereby separatedaccording to their charge and molecular weight. This method ofseparation is well known to those skilled in the art and is typicallyapplied to separating various forms of enzymes and of DNA fragmentsproduced by restriction endonucleases.

Emasculate refers to the removal of plant male sex organs.

Enzymes are organic catalysts that can exist in various forms calledisozymes.

F₁ Hybrid refers to the first generation progeny of the cross of twoplants.

Genetic Complement refers to an aggregate of nucleotide sequences, theexpression of which sequences defines the phenotype in corn plants, orcomponents of plants including cells or tissue.

Genotype refers to the genetic constitution of a cell or organism.

Haploid refers to a cell or organism having one set of the two sets ofchromosomes in a diploid.

Isozymes are one of a number of enzymes which catalyze the samereaction(s) but differ from each other, e.g. in primary structure and/orelectrophoretic mobility. The differences between isozymes are undersingle gene, codominant control. Consequently, electrophoreticseparation to produce band patterns can be equated to different allelesat the DNA level. Structural differences that do not alter charge cannotbe detected by this method.

Isozyme typing profile refers to a profile of band patterns of isozymesseparated by electrophoresis that can be equated to different alleles atthe DNA level.

Linkage refers to a phenomenon wherein alleles on the same chromosometend to segregate together more often than expected by chance if theirtransmission was independent.

Marker is a readily detectable phenotype, preferably inherited incodominant fashion (both alleles at a locus in a diploid heterozygoteare readily detectable), with no environmental variance component, i.e.,heritability of 1.

85DGD1 refers to the corn plant from which seeds having ATCC AccessionNo. PTA-6160 were obtained, as well as plants grown from those seeds.

Phenotype refers to the detectable characteristics of a cell ororganism, which characteristics are the manifestation of geneexpression.

Quantitative Trait Loci (QTL) refer to genetic loci that control to somedegree numerically representable traits that are usually continuouslydistributed.

Regeneration refers to the development of a plant from tissue culture.

RFLP genetic marker profile refers to a profile of band patterns of DNAfragment lengths typically separated by agarose gel electrophoresis,after restriction endonuclease digestion of DNA.

Self-pollination refers to the transfer of pollen from the anther to thestigma of the same plant.

Single Gene Converted (Conversion) Plant refers to plants which aredeveloped by a plant breeding technique called backcrossing whereinessentially all of the desired morphological and physiologicalcharacteristics of an inbred are recovered in addition to the singlegene transferred into the inbred via the backcrossing technique.

Tissue Culture refers to a composition comprising isolated cells of thesame or a different type or a collection of such cells organized intoparts of a plant.

The following examples are included to demonstrate preferred embodimentsof the invention. It should be appreciated by those of skill in the artthat the techniques disclosed in the examples that follow representtechniques discovered by the inventor to function well in the practiceof the invention, and thus can be considered to constitute preferredmodes for its practice. However, those of skill in the art should, inlight of the present disclosure, appreciate that many changes can bemade in the specific embodiments that are disclosed and still obtain alike or similar result without departing from the spirit and scope ofthe invention.

III. Inbred Corn Plant GMLEA

In accordance with one aspect of the present invention, there isprovided a novel inbred corn plant, designated GMLEA. Inbred corn plantGMLEA is a yellow, dent corn inbred that can be compared to inbred cornplants 88145 and 8M116, proprietary inbreds of DEKALB GeneticsCorporation, and the single cross parent 6M502.6M502A. GMLEA differssignificantly (at the 5% level) from these inbred lines in severalaspects (Table 1A, Table 1B, Table 1C).

TABLE 1A COMPARISON OF GMLEA WITH 88145 BARREN DROP EHT MST PHT RTL SHEDSILK STL YLD INBRED % % INCH FINAL % INCH % GDU GDU % BU/A GMLEA 0.6 0.042.3 59.4 22.9 86.4 0.3 1662.5 1680.1 4.3 79.0 88145 0.3 0.1 42.9 59.524.1 87.5 6.2 1635.5 1679.9 1.7 57.8 DIFF 0.3 −0.1 −0.6 −0.1 −1.2 −1.1−5.9 27.0 0.5 2.6 21.2 # 6 8 8 8 7 8 8 8 8 8 8 LOC/TESTS P VALUE 0.940.81 0.63 0.89 0.36 0.46 0.00** 0.04* 0.96 0.04* 0.00** LegendAbbreviations BARREN % = Barren Plants (Percent) DROP % = Dropped Ears(Percent) EHT INCH = Ear Height (Inches) FINAL = Final Stand MST % =Moisture (Percent) PHT INCH = Plant Height (Inches) RTL % = Root Lodging(Percent) SHED GDU = GDUs to Shed SILK GDU = GDUs to Silk STL % = StalkLodging (Percent) YLD BU/A = Yield (Bushels/Acre) Significance levelsare indicated as: *5 percent **1 percent

TABLE 1B COMPARISON OF GMLEA WITH 8M116 BARREN DROP EHT MST PHT RTL SHEDSILK STL YLD INBRED % % INCH FINAL % INCH % GDU GDU % BU/A GMLEA 0.6 0.042.3 59.4 22.9 86.4 0.3 1662.5 1680.1 4.3 79.0 8M116 0.2 0.3 37.9 59.721.6 88.6 0.5 1598.3 1650.8 3.6 70.7 DIFF 0.4 −0.3 4.3 −0.3 1.4 −2.3−0.2 64.3 29.3 0.7 8.3 # 6 8 8 8 7 8 8 8 8 8 8 LOC/TESTS P VALUE 0.900.48 0.00** 0.81 0.29 0.17 0.85 0.00** 0.02* 0.53 0.23 LegendAbbreviations BARREN % = Barren Plants (Percent) DROP % = Dropped Ears(Percent) EHT INCH = Ear Height (Inches) FINAL = Final Stand MST % =Moisture (Percent) PHT INCH = Plant Height (Inches) RTL % = Root Lodging(Percent) SHED GDU = GDUs to Shed SILK GDU = GDUs to Silk STL % = StalkLodging (Percent) YLD BU/A = Yield (Bushels/Acre) Significance levelsare indicated as: *5 percent **1 percent

TABLE 1C COMPARISON OF GMLEA WITH 6M502.6M502A BARREN DROP EHT MST PHTRTL SHED SILK STL YLD INBRED % % INCH FINAL % INCH % GDU GDU % BU/AGMLEA 0.6 0.0 42.3 59.4 22.9 86.4 0.3 1662.5 1680.1 4.3 79.0 6M502. 0.30.4 35.4 59.5 24.2 79.9 0.1 1540.6 1544.2 7.2 90.2 6M502A DIFF 0.2 −0.46.8 −0.1 −1.3 6.4 0.1 121.9 135.9 −2.9 −11.2 # 6 8 8 8 7 8 8 8 8 8 8LOC/TESTS P VALUE 0.87 0.21 0.00** 0.95 0.22 0.00** 0.94 0.00** 0.00**0.01** 0.06+ Legend Abbreviations BARREN % = Barren Plants (Percent)DROP % = Dropped Ears (Percent) EHT INCH = Ear Height (Inches) FINAL =Final Stand MST % = Moisture (Percent) PHT INCH = Plant Height (Inches)RTL % = Root Lodging (Percent) SHED GDU = GDUs to Shed SILK GDU = GDUsto Silk STL % = Stalk Lodging (Percent) YLD BU/A = Yield (Bushels/Acre)Significance levels are indicated as: +10 percent **1 percent

A. Origin and Breeding History

Inbred plant GMLEA was derived from the cross of three way cross ofinbred lines designated MO17HT (a publicly available inbred), 75802 (aproprietary inbred developed by DEKALB Genetics Corporation) and LH38 (aline developed by Holden's Foundation Seeds, Inc.).

GMLEA shows uniformity and stability within the limits of environmentalinfluence for the traits described hereinafter in Table 2. GMLEA hasbeen self-pollinated and ear-rowed a sufficient number of generationswith careful attention paid to uniformity of plant type to ensurehomozygosity and phenotypic stability. No variant traits have beenobserved or are expected in GMLEA.

Inbred corn plants can be reproduced by planting such inbred seeds,growing the resulting corn plants under self-pollinating orsib-pollinating conditions with adequate isolation using standardtechniques well known to an artisan skilled in the agricultural arts.Seeds can be harvested from such a plant using standard, well knownprocedures.

The origin and breeding history of inbred plant GMLEA can be summarizedas follows:

Winter 1982

-   -   The cross of LH38 to the cross between MO17HT.75802 was made        (nursery row numbers 215 and 216).

Winter 1983

-   -   LH38, a line developed by Holden's Foundation Seeds, Inc., was        crossed to the single cross described above (equivalent to S₁        generation) (nursery row numbers 35 and 36).

Summer 1984

-   -   The S₁ was grown and plants were self-pollinated (nursery row        number 1309-1312).

Winter 1985

-   -   S₂ ears were grown ear-to-row and self-pollinated (nursery rows        85-90).

Summer 1985

-   -   S₃ ears were grown ear-to-row and self-pollinated (nursery rows        2415-2418).

Winter 1986

-   -   S₄ ears were grown ear-to-row and self-pollinated (nursery rows        98-101).

Summer 1986

-   -   S₅ ears were grown ear-to-row and self-pollinated (nursery rows        4491-4493).

Summer 1987

-   -   S₆ ears were grown ear-to-row and self-pollinated (nursery rows        5247-5248). Three ears were selected from row 5247, bulked, and        designated GMLEA at harvest.

B. Phenotypic Description

In accordance with another aspect of the present invention, there isprovided a corn plant having the functional and morphologicalcharacteristics of corn plant GMLEA. A description of the functional andmorphological characteristics of corn plant GMLEA is presented in Table2.

TABLE 2 MORPHOLOGICAL TRAITS FOR THE GMLEA PHENOTYPE YEAR OF DATA: 1992,1993 CHARACTERISTIC VALUE* 1. Stalk Diameter (Width) cm. 2.4 AnthocyaninABSENT Nodes With Brace Roots 2.8 Brace Root Color GREEN InternodeDirection STRAIGHT Internode Length cm. 18.9 2. Leaf Angle UPRIGHTNumber 19.1 Color MEDIUM GREEN Length cm. 73.8 Width cm. 9.8 SheathPubescence LIGHT Marginal Waves FEW 3. Tassel Length cm. 35.6 SpikeLength cm. 19.3 Peduncle Length cm. 9.5 Attitude COMPACT Branch AngleUPRIGHT Branch Number 6.7 Glume Color GREEN Glume Band ABSENT 4. EarSilk Color PINK Number Per Stalk 1.1 Position (Attitude) UPRIGHT Lengthcm. 18.3 Shape SEMI-CONICAL Diameter cm. 37.8 Weight gm. 126.8 ShankLength cm. 10.8 Shank Internode Number 7.7 Husk Bract SHORT Husk Covercm. 2.0 Husk Color Fresh GREEN Husk Color Dry BUFF Cob Diameter cm. 22.0Cob Color WHITE Shelling Percent 86.0 5. Kernel Row Number 14.4 NumberPer Row 33.3 Row Direction CURVED Type DENT Cap Color YELLOW Side ColorORANGE Length (Depth) mm. 10.1 Width mm. 7.4 Thickness 4.6 Weight of1000K gm. 276.5 Endosperm Type NORMAL Endosperm Color YELLOW *These aretypical values. Values may vary due to environment. Other values thatare substantially equivalent are also within the scope of the invention.Substantially equivalent refers to quantitative traits that whencompared do not show statistical differences of their means.IV. Inbred Corn Plant 6F905

In accordance with another aspect of the present invention, there isprovided a novel inbred corn plant, designated 6F905. Inbred corn plant6F905 is a yellow, dent corn inbred that can be compared to inbred cornplants F118, LH132, and LH195. F118 is a proprietary inbred of DEKALBGenetics Corporation. LH132 and LH195 are inbreds developed by Holden'sFoundation Seeds, Inc. 6F905 differs significantly (at the 5% level)from these inbred lines in several aspects (Table 3A, Table 3B, Table3C).

TABLE 3A COMPARISON OF 6F905 WITH F118 BARREN DROP EHT MST PHT RTL SHEDSILK STL YLD INBRED % % INCH FINAL % INCH % GDU GDU % BU/A 6F905 0.0 0.335.6 58.5 26.9 78.3 0.0 1560.6 1588.1 3.2 91.9 F118 1.1 0.0 32.9 58.627.9 75.5 0.1 1640.6 1652.4 3.1 68.3 DIFF −1.1 0.3 2.7 −0.1 −1.0 2.8−0.1 −80.1 −64.3 0.1 23.6 # 6 8 8 8 8 8 8 8 8 8 8 LOC/TESTS P VALUE 0.770.31 0.05+ 0.85 0.42 0.08+ 0.94 0.00** 0.00** 0.99 0.00** LegendAbbreviations BARREN % = Barren Plants (Percent) DROP % = Dropped Ears(Percent) EHT INCH = Ear Height (Inches) FINAL = Final Stand MST % =Moisture (Percent) PHT INCH = Plant Height (Inches) RTL % = Root Lodging(Percent) SHED GDU = GDUs to Shed SILK GDU = GDUs to Silk STL % = StalkLodging (Percent) YLD BU/A = Yield (Bushels/Acre) Significance levelsare indicated as: +10 percent **1 percent

TABLE 3B COMPARISON OF 6F905 WITH LH132 BARREN DROP EHT MST PHT RTL SHEDSILK STL YLD INBRED % % INCH FINAL % INCH % GDU GDU % BU/A 6F905 0.9 0.235.4 58.5 28.2 75.9 0.1 1592.8 1619.0 3.4 77.5 LH132 2.3 0.1 28.0 58.217.8 71.4 0.0 1512.4 1516.7 1.3 73.0 DIFF −1.5 0.1 7.4 0.3 10.4 4.6 0.180.4 102.3 2.2 4.5 # 14 16 16 16 15 16 16 16 16 16 16 LOC/TESTS P VALUE0.54 0.57 0.00** 0.87 0.00** 0.00** 0.95 0.00** 0.00** 0.07+ 0.46 LegendAbbreviations BARREN % = Barren Plants (Percent) DROP % = Dropped Ears(Percent) EHT INCH = Ear Height (Inches) FINAL = Final Stand MST % =Moisture (Percent) PHT INCH = Plant Height (Inches) RTL % = Root Lodging(Percent) SHED GDU = GDUs to Shed SILK GDU = GDUs to Silk STL % = StalkLodging (Percent) YLD BU/A = Yield (Bushels/Acre) Significance levelsare indicated as: +10 percent **1 percent

TABLE 3C COMPARISON OF 6F905 WITH LH195 BARREN DROP EHT MST PHT RTL SHEDSILK STL YLD INBRED % % INCH FINAL % INCH % GDU GDU % BU/A 6F905 1.2 0.234.4 58.8 27.5 74.6 0.1 1591.7 1616.1 3.5 77.2 LH195 1.7 0.0 25.3 58.422.7 62.2 0.0 1623.2 1625.7 0.8 64.1 DIFF −0.5 0.2 9.1 0.3 4.7 12.4 0.1−31.5 −9.6 2.7 13.1 # 10 12 12 12 10 12 12 12 12 12 12 LOC/TESTS P VALUE0.86 0.40 0.00** 0.86 0.00** 0.00** 0.94 0.00** 0.47 0.05* 0.01** LegendAbbreviations BARREN % = Barren Plants (Percent) DROP % = Dropped Ears(Percent) EHT INCH = Ear Height (Inches) FINAL = Final Stand MST % =Moisture (Percent) PHT INCH = Plant Height (Inches) RTL % = Root Lodging(Percent) SHED GDU = GDUs to Shed SILK GDU = GDUs to Silk STL % = StalkLodging (Percent) YLD BU/A = Yield (Bushels/Acre) Significance levelsare indicated as: *5 percent **1 percent

A. Origin and Breeding History

Inbred plant 6F905 was derived from the cross of two inbred plantsdesignated 4682 and 88051B (both proprietary inbreds of DEKALB GeneticsCorporation) in the summer of 1982.

6F905 shows uniformity and stability within the limits of environmentalinfluence for the traits described hereinafter in Table 4. 6F905 hasbeen self-pollinated and ear-rowed a sufficient number of generationswith careful attention paid to uniformity of plant type to ensurehomozygosity and phenotypic stability. No variant traits have beenobserved or are expected in 6F905.

Inbred corn plants can be reproduced by planting such inbred seeds,growing the resulting corn plants under self-pollinating orsib-pollinating conditions with adequate isolation using standardtechniques well known to an artisan skilled in the agricultural arts.Seeds can be harvested from such a plant using standard, well knownprocedures.

The origin and breeding history of inbred plant 6F905 can be summarizedas follows:

Summer 1982

-   -   The cross 4682×88051B was made (nursery book row numbers 707,        708).

Winter 1982

-   -   S₀ seed was grown and selfed (nursery row number 6108).

Summer 1983

-   -   Bulked S₁ seed was grown and plants were selfed (nursery row        number 1823).

Summer 1984

-   -   S₂ ears were grown ear-to-row and plants were selfed (nursery        row numbers 1224 to 1235).

Summer 1985

-   -   S₃ ears were grown ear-to-row and plants were selfed (nursery        row number 1232).

Summer 1987

-   -   S₄ ears were grown ear-to-row (nursery row number 3802).

Summer 1988

-   -   S₅ ears were grown (nursery row number 2879).

Winter 1988

-   -   S₆ ears were grown (nursery row numbers 4105-4106).

Winter 1989

-   -   S₇ ear was grown (nursery row number 4615). S₈ ears were        selected and coded 6F905.

B. Phenotypic Description

In accordance with another aspect of the present invention, there isprovided a corn plant having the functional and morphologicalcharacteristics of corn plant 6F905. A description of the functional andmorphological characteristics of corn plant 6F905 is presented in Table4.

TABLE 4 MORPHOLOGICAL TRAITS FOR THE 6F905 PHENOTYPE YEAR OF DATA: 1992,1993 CHARACTERISTIC VALUE* 1. Stalk Diameter (Width) cm. 2.7 AnthocyaninBASAL WEAK Nodes With Brace Roots 2.5 Brace Root Color PURPLE InternodeDirection STRAIGHT Internode Length cm. 15.2 2. Leaf Number 19.1 ColorDARK GREEN Length cm. 80.6 Width cm. 10.1 Sheath Pubescence HEAVYMarginal Waves FEW 3. Tassel Length cm. 36.9 Spike Length cm. 22.0Peduncle Length cm. 8.8 Attitude COMPACT Branch Angle UPRIGHT BranchNumber 9.0 Anther Color PINK Glume Band ABSENT 4. Ear Silk Color PINKNumber Per Stalk 1.1 Position (Attitude) UPRIGHT Length cm. 14.8 ShapeSEMI-CONICAL Diameter cm. 45.8 Weight gm. 159.4 Shank Length cm. 8.7Shank Internode Number 8.3 Husk Bract SHORT Husk Cover cm. 9.0 HuskOpening TIGHT Husk Color Fresh GREEN Husk Color Dry BUFF Cob Diametercm. 27.2 Shelling Percent 82.8 5. Kernel Row Number 15.7 Number Per Row34.5 Row Direction CURVED Type DENT Cap Color YELLOW Side Color ORANGELength (Depth) mm. 12.2 Width mm. 8.0 Thickness 3.8 Weight of 1000K gm.273.0 Endosperm Type NORMAL Endosperm Color YELLOW *These are typicalvalues. Values may vary due to environment. Other values that aresubstantially equivalent are also within the scope of the invention.Substantially equivalent refers to quantitative traits that whencompared do not show statistical differences of their means.V. Inbred Corn Plant 91CSV-1

In accordance with another aspect of the present invention, there isprovided a novel inbred corn plant, designated 91CSV-1. Inbred cornplant 91CSV-1 is a yellow, dent corn inbred that can be compared toinbred corn plants 3IIH6, 78551S, 83IBI3, and FBLA, all proprietaryinbreds of DEKALB Genetics Corporation. 91CSV-1 differs significantly(at the 5% level) from these inbred lines in several aspects (Table 5A,Table 5B, Table 5C, Table 5D).

TABLE 5A COMPARISON OF 91CSV-1 WITH 3IIH6 BARREN DROP EHT MST PHT RTLSHED SILK STL YLD INBRED % % INCH FINAL % INCH % GDU GDU % BU/A 91CSV-11.2 0.3 31.1 57.8 16.9 70.7 5.4 1379.0 1453.1 4.3 68.1 3IIH6 1.0 0.727.0 58.2 18.8 64.3 1.4 1449.6 1469.4 5.4 69.1 DIFF 0.2 −0.4 4.1 −0.4−1.9 6.4 4.1 −70.6 −16.2 −1.1 −1.0 # 30 32 35 35 32 35 32 35 35 34 35LOC/TESTS P VALUE 0.98 0.16 0.00** 0.52 0.00** 0.00** 0.01** 0.00**0.02* 0.98 0.84 Legend Abbreviations BARREN % = Barren Plants (Percent)DROP % = Dropped Ears (Percent) EHT INCH = Ear Height (Inches) FINAL =Final Stand MST % = Moisture (Percent) PHT INCH = Plant Height (Inches)RTL % = Root Lodging (Percent) SHED GDU = GDUs to Shed SILK GDU = GDUsto Silk STL % = Stalk Lodging (Percent) YLD BU/A = Yield (Bushels/Acre)Significance levels are indicated as: *5 percent **1 percent

TABLE 5B COMPARISON OF 91CSV-1 WITH 78551S BARREN DROP EHT MST PHT RTLSHED SILK STL YLD INBRED % % INCH FINAL % INCH % GDU GDU % BU/A 91CSV-11.1 0.4 31.7 57.7 16.6 71.5 3.4 1374.2 1447.5 3.1 70.1 78551S 1.3 0.423.3 54.9 19.0 67.0 0.6 1408.7 1469.2 2.6 65.9 DIFF −0.2 0.0 8.3 2.8−2.3 4.6 2.7 −34.5 −21.7 0.5 4.2 # 24 26 30 30 27 30 26 30 30 28 30LOC/TESTS P VALUE 0.87 0.82 0.00** 0.01** 0.00** 0.00** 0.00** 0.00**0.01** 0.40 0.18 Legend Abbreviations BARREN % = Barren Plants (Percent)DROP % = Dropped Ears (Percent) EHT INCH = Ear Height (Inches) FINAL =Final Stand MST % = Moisture (Percent) PHT INCH = Plant Height (Inches)RTL % = Root Lodging (Percent) SHED GDU = GDUs to Shed SILK GDU = GDUsto Silk STL % = Stalk Lodging (Percent) YLD BU/A = Yield (Bushels/Acre)Significance levels are indicated as: **1 percent

TABLE 5C COMPARISON OF 91CSV-1 WITH 83IBI3 BARREN DROP EHT MST PHT RTLSHED SILK STL YLD INBRED % % INCH FINAL % INCH % GDU GDU % BU/A 91CSV-11.2 0.3 31.1 57.8 16.8 70.7 5.5 1379.0 1453.1 4.3 68.1 83IBI3 1.1 0.227.7 58.8 17.9 59.9 1.3 1391.9 1429.5 2.9 65.4 DIFF 0.1 0.1 3.5 −1.0−1.1 10.8 4.2 −12.9 23.6 1.5 2.7 # 30 31 35 35 33 35 31 35 35 33 35LOC/TESTS P VALUE 0.82 0.86 0.00** 0.22 0.11 0.00** 0.00** 0.07+ 0.00**0.17 0.31 Legend Abbreviations BARREN % = Barren Plants (Percent) DROP %= Dropped Ears (Percent) EHT INCH = Ear Height (Inches) FINAL = FinalStand MST % = Moisture (Percent) PHT INCH = Plant Height (Inches) RTL %= Root Lodging (Percent) SHED GDU = GDUs to Shed SILK GDU = GDUs to SilkSTL % = Stalk Lodging (Percent) YLD BU/A = Yield (Bushels/Acre)Significance levels are indicated as: +10 percent **1 percent

TABLE 5D COMPARISON OF 91CSV-1 WITH FBLA BARREN DROP EHT MST PHT RTLSHED SILK STL YLD INBRED % % INCH FINAL % INCH % GDU GDU % BU/A 91CSV-10.9 0.2 32.4 57.8 17.8 72.7 6.2 1368.2 1437.6 4.7 73.3 FBLA 0.5 0.4 27.058.7 18.6 66.5 2.3 1445.8 1464.3 1.0 65.4 DIFF 0.4 −0.2 5.4 −0.9 −0.86.2 3.9 −77.6 −26.8 3.8 7.9 # 21 25 27 27 27 27 25 27 27 26 27 LOC/TESTSP VALUE 0.61 0.35 0.00** 0.43 0.29 0.00** 0.01** 0.00** 0.00** 0.02*0.02* Legend Abbreviations BARREN % = Barren Plants (Percent) DROP % =Dropped Ears (Percent) EHT INCH = Ear Height (Inches) FINAL = FinalStand MST % = Moisture (Percent) PHT INCH = Plant Height (Inches) RTL %= Root Lodging (Percent) SHED GDU = GDUs to Shed SILK GDU = GDUs to SilkSTL % = Stalk Lodging (Percent) YLD BU/A = Yield (Bushels/Acre)Significance levels are indicated as: *5 percent **1 percent

A. Origin and Breeding History

Inbred plant 91CSV-1 was derived from the cross between the inbreddesignated 78551S (proprietary inbred developed by DEKALB GeneticsCorporation; Ser. No. 08/181,710, filed Jan. 14, 1994; and a lineselected from Pioneer hybrid 3953.

91CSV-1 shows uniformity and stability within the limits ofenvironmental influence for the traits described hereinafter in Table 6.91CSV-1 has been self-pollinated and ear-rowed a sufficient number ofgenerations with careful attention paid to uniformity of plant type toensure homozygosity and phenotypic stability. No variant traits havebeen observed or are expected in 91CSV-1.

Inbred corn plants can be reproduced by planting such inbred seeds,growing the resulting corn plants under self-pollinating orsib-pollinating conditions with adequate isolation using standardtechniques well known to an artisan skilled in the agricultural arts.Seeds can be harvested from such a plant using standard, well knownprocedures.

The origin and breeding history of inbred plant 91CSV-1 can besummarized as follows:

Spring 1987

-   -   The inbred 78551S (a proprietary inbred of DEKALB Genetics        Corporation) was crossed to a selection from Pioneer hybrid 3953        to generate the S₀ generation.

Summer 1987

-   -   The S₀ was crossed to 78551S to generate the BC₁S₁.

Winter 1988

-   -   BC₁S₁ seed was grown and plants were self-pollinated (nursery        rows 313-11 to 15).

Summer 1988

-   -   S₂ ears were grown ear-to-row (nursery rows 81-52 to 81-91).

Summer 1989

-   -   S₃ ears were grown ear-to-row (nursery rows 2-1 to 2-18 and 3-1        to 3-42).

Winter 1990

-   -   S₄ ears were grown ear-to-row (nursery rows 69-54 to 56).

Summer 1990

-   -   S₅ ears grown ear-to-row (nursery rows 304-25 to 304-42),        selfed, seed was bulked after harvest, and given the designation        91CSV-1.

B. Phenotypic Description

In accordance with another aspect of the present invention, there isprovided a corn plant having the functional and morphologicalcharacteristics of corn plant 91CSV-1. A description of the functionaland morphological characteristics of corn plant 91CSV-1 is presented inTable 6.

TABLE 6 MORPHOLOGICAL TRAITS FOR THE 91CSV-1 PHENOTYPE YEAR OF DATA:1991, 1992 CHARACTERISTIC VALUE* 1. Stalk Diameter (Width) cm. 1.9Anthocyanin ABSENT Nodes With Brace Roots 1.3 Brace Root Color GREENInternode Direction STRAIGHT Internode Length cm. 13.1 2. Leaf Number17.3 Color MEDIUM GREEN Length cm. 75.7 Width cm. 8.7 Sheath AnthocyaninABSENT Longitudinal Creases FEW 3. Tassel Length cm. 32.3 Spike Lengthcm. 25.3 Peduncle Length cm. 8.4 Branch Angle INTERMEDIATE Branch Number7.4 Anther Color GREEN-YELLOW Glume Color GREEN Glume Band ABSENT 4. EarSilk Color GREEN-YELLOW Number Per Stalk 1.1 Position (Attitude) UPRIGHTLength cm. 15.9 Shape SEMI-CONICAL Diameter cm. 36.3 Weight gm. 84.9Shank Length cm. 14.0 Shank Internode Number 5.4 Husk Bract SHORT HuskCover cm. 7.0 Husk Opening INTERMEDIATE Husk Color Fresh GREEN HuskColor Dry BUFF Cob Diameter cm. 20.4 Cob Color RED Cob Strength WEAKShelling Percent 83.5 5. Kernel Row Number 13.3 Number Per Row 33.6 RowDirection CURVED Type DENT Cap Color YELLOW Side Color DEEP YELLOWLength (Depth) mm. 9.4 Width mm. 8.3 Thickness 4.3 Weight of 1000K gm.194.2 Endosperm Type NORMAL Endosperm Color YELLOW *These are typicalvalues. Values may vary due to environment. Other values that aresubstantially equivalent are also within the scope of the invention.Substantially equivalent refers to quantitative traits that whencompared do not show statistical differences of their means.VI. Inbred Corn Plant 91DFA-5

In accordance with another aspect of the present invention, there isprovided a novel inbred corn plant, designated 91DFA-5. Inbred cornplant 91DFA-5 is a yellow, dent corn inbred that can be compared toinbred corn plants 2FACC, 2FADB, 78010, and FBAB, all of which areproprietary inbreds developed by DEKALB Genetics Corporation. 91DFA-5differs significantly (at the 5% level) from these inbred lines inseveral aspects (Table 7A, Table 7B, Table 7C, Table 7D).

TABLE 7A COMPARISON OF 91DFA-5 WITH 2FADB BARREN DROP EHT MST PHT RTLSHED SILK STL YLD INBRED % % INCH FINAL % INCH % GDU GDU % BU/A 91DFA-51.4 0.7 34.0 58.8 18.8 69.2 0.1 1506.0 1511.7 5.4 61.5 2FADB 0.4 0.328.0 58.6 21.4 66.8 2.5 1490.4 1484.5 2.9 81.2 DIFF 1.0 0.4 6.0 0.3 −2.52.4 −2.4 15.6 27.2 2.5 −19.7 # 30 30 32 33 31 32 31 33 33 32 33LOC/TESTS P VALUE 0.41 0.08+ 0.00** 0.86 0.00** 0.00** 0.38 0.02* 0.00**0.34 0.00** Legend Abbreviations BARREN % = Barren Plants (Percent) DROP% = Dropped Ears (Percent) EHT INCH = Ear Height (Inches) FINAL = FinalStand MST % = Moisture (Percent) PHT INCH = Plant Height (Inches) RTL %= Root Lodging (Percent) SHED GDU = GDUs to Shed SILK GDU = GDUs to SilkSTL % = Stalk Lodging (Percent) YLD BU/A = Yield (Bushels/Acre)Significance levels are indicated as: *5 percent **1 percent

TABLE 7B COMPARISON OF 91DFA-5 WITH 2FADB BARREN DROP EHT MST PHT RTLSHED SILK STL YLD INBRED % % INCH FINAL % INCH % GDU GDU % BU/A 91DFA-51.2 0.8 33.7 59.4 19.0 68.1 0.1 1508.3 1512.2 6.0 58.8 2FACC 0.4 0.328.1 58.2 22.5 67.4 2.8 1504.8 1503.2 2.2 82.5 DIFF 0.8 0.4 5.6 12 −3.60.7 −2.8 3.5 8.9 3.8 −23.7 # 26 26 28 28 26 28 26 28 28 28 27 LOC/TESTSP VALUE 0.48 0.07+ 0.00** 0.14 0.00** 0.41 0.48 0.62 0.25 0.09+ 0.00**Legend Abbreviations BARREN % = Barren Plants (Percent) DROP % = DroppedEars (Percent) EHT INCH = Ear Height (Inches) FINAL = Final Stand MST %= Moisture (Percent) PHT INCH = Plant Height (Inches) RTL % = RootLodging (Percent) SHED GDU = GDUs to Shed SILK GDU = GDUs to Silk STL %= Stalk Lodging (Percent) YLD BU/A = Yield (Bushels/Acre) Significancelevels are indicated as: +10 percent *5 percent **1 percent

TABLE 7C COMPARISON OF 91DFA-5 WITH 78010 BARREN DROP EHT MST PHT RTLSHED SILK STL YLD INBRED % % INCH FINAL % INCH % GDU GDU % BU/A 91DFA-51.3 0.5 34.5 58.5 19.9 70.0 0.1 1496.5 1505.1 5.0 64.8 78010 0.6 0.428.7 58.9 21.8 58.9 1.3 1478.6 1498.8 1.6 70.4 DIFF 0.7 0.1 5.9 −0.4−1.8 11.1 −1.2 17.9 6.3 3.4 −5.6 # 21 24 24 25 25 24 25 25 25 25 25LOC/TESTS P VALUE 0.53 0.39 0.00** 0.69 0.01** 0.00** 0.71 0.01** 0.320.14 0.13 Legend Abbreviations BARREN % = Barren Plants (Percent) DROP %= Dropped Ears (Percent) EHT INCH = Ear Height (Inches) FINAL = FinalStand MST % = Moisture (Percent) PHT INCH = Plant Height (Inches) RTL %= Root Lodging (Percent) SHED GDU = GDUs to Shed SILK GDU = GDUs to SilkSTL % = Stalk Lodging (Percent) YLD BU/A = Yield (Bushels/Acre)Significance levels are indicated as: **1 percent

TABLE 7D COMPARISON OF 91DFA-5 WITH FBAB BARREN DROP EHT MST PHT RTLSHED SILK STL YLD INBRED % % INCH FINAL % INCH % GDU GDU % BU/A 91DFA-51.3 0.6 33.9 58.5 19.7 69.3 0.2 1498.7 1505.3 4.9 62.0 FBAB 2.5 0.5 28.758.4 17.1 74.3 1.5 1434.7 1451.6 13.6 48.0 DIFF −1.2 0.1 5.2 0.0 2.6−5.0 −1.3 64.0 53.8 −8.7 14.0 # 34 37 39 40 32 39 37 40 40 39 40LOC/TESTS P VALUE 0.16 0.56 0.00** 0.94 0.00** 0.00** 0.01** 0.00**0.00** 0.00** 0.00** Legend Abbreviations BARREN % = Barren Plants(Percent) DROP % = Dropped Ears (Percent) EHT INCH = Ear Height (Inches)FINAL = Final Stand MST % = Moisture (Percent) PHT INCH = Plant Height(Inches) RTL % = Root Lodging (Percent) SHED GDU = GDUs to Shed SILK GDU= GDUs to Silk STL % = Stalk Lodging (Percent) YLD BU/A = Yield(Bushels/Acre) Significance levels are indicated as: **1 percent

A. Origin and Breeding History

Inbred plant 91DFA-5 was derived from the cross of two inbred plantsdesignated 78010 and FBAB in the summer of 1985. Both 78010 and FBAB areproprietary inbreds developed by DEKALB Genetics Corporation.

91DFA-5 shows uniformity and stability within the limits ofenvironmental influence for the traits described hereinafter in Table 8.91DFA-5 has been self-pollinated and ear-rowed a sufficient number ofgenerations with careful attention paid to uniformity of plant type toensure homozygosity and phenotypic stability. No variant traits havebeen observed or are expected in 91DFA-5.

Inbred corn plants can be reproduced by planting such inbred seeds,growing the resulting corn plants under self-pollinating orsib-pollinating conditions with adequate isolation using standardtechniques well known to an artisan skilled in the agricultural arts.Seeds can be harvested from such a plant using standard, well knownprocedures.

The origin and breeding history of inbred plant 91DFA-5 can besummarized as follows:

Summer 1985

-   -   The inbred 78010 was crossed to the inbred FBAB (both        proprietary inbreds of DEKALB Genetics Corporation).

Summer 1986

-   -   The S₀ was backcrossed to 78010 (nursery range 115, rows 100        and 101) to generate the BC₁S₁.

Summer 1987

-   -   The BC₁S₁ seed was grown and plants were self-pollinated        (nursery rows 8-67 to 8-74).

Winter 1988

-   -   S₂ ears were grown ear-to-row (nursery rows 545-45 to 62).

Summer 1988

-   -   S₃ ears were grown ear-to-row (nursery rows 56-26 to 56-56).

Winter 1989

-   -   S₄ ears were grown ear-to-row (nursery rows 74-17 to 74-19).

Summer 1989

-   -   S₅ ears were grown ear-to-row (nursery rows 32-61 to 32-60),        bulked, and given the designation 91DFA-5.

B. Phenotypic Description

In accordance with another Aspect of the present invention, there isprovided a corn plant having the functional and morphologicalcharacteristics of corn plant 91DFA-5. A description of the functionaland morphological characteristics of corn plant 91DFA-5 is presented inTable 8.

TABLE 8 MORPHOLOGICAL TRAITS FOR THE 91DFA-5 PHENOTYPE YEAR OF DATA:1991, 1992, 1993 CHARACTERISTIC VALUE* 1. Stalk Diameter (Width) cm. 1.9Nodes With Brace Roots 1.2 Brace Root Color RED Internode DirectionSTRAIGHT Internode Length cm. 14.0 2. Leaf Number 19.3 Color MEDIUMGREEN Length cm. 67.3 Width cm. 8.3 Sheath Pubescence MEDIUM 3. TasselLength cm. 29.3 Spike Length cm. 20.8 Peduncle Length cm. 8.1 BranchAngle INTERMEDIATE Branch Number 5.4 Glume Color GREEN Glume Band ABSENT4. Ear Number Per Stalk 1.1 Position (Attitude) UPRIGHT Length cm. 14.6Shape SEMI-CONICAL Diameter cm. 36.8 Weight gm. 97.1 Shank Length cm.12.6 Shank Internode Number 5.2 Husk Bract SHORT Husk Cover cm. 3.4 HuskOpening INTERMEDIATE Husk Color Fresh GREEN Husk Color Dry BUFF CobDiameter cm. 21.4 Cob Strength STRONG Shelling Percent 80.2 5. KernelRow Number 13.8 Number Per Row 26.3 Row Direction CURVED Cap ColorYELLOW Length (Depth) mm. 9.9 Width mm. 7.9 Thickness 4.5 Weight of1000K gm. 246.6 Endosperm Type NORMAL Endosperm Color YELLOW *These aretypical values. Values may vary due to environment. Other values thatare substantially equivalent are also within the scope of the invention.Substantially equivalent refers to quantitative traits that whencompared do not show statistical differences of their means.VII. Inbred Corn Plant WDAQ2

In accordance with another aspect of the present invention, there isprovided a novel inbred corn plant, designated WDAQ2. Inbred corn plantWDAQ2 is a yellow, dent corn inbred that can be compared to inbred cornplants B73HT, FBLL, and WDAD1. B73HT is a public inbred, and FBLL andWDAD1 are proprietary inbreds developed by DEKALB Genetics Corporation.WDAQ2 differs significantly (at the 5% level) from these inbred lines inseveral aspects (Table 9A, Table 9B, Table 9C).

TABLE 9A COMPARISON OF WDAQ2 WITH B73HT BARREN DROP EHT MST PHT RTL SHEDSILK STL YLD INBRED % % INCH FINAL % INCH % GDU GDU % BU/A WDAQ2 1.0 0.139.8 56.5 20.3 78.6 0.0 1650.7 1661.2 3.5 71.5 B73HT 1.4 0.7 39.5 57.123.6 82.3 0.3 1638.7 1634.5 2.2 61.9 DIFF −0.3 −0.5 0.3 −0.6 −3.3 −3.6−0.3 12.0 26.8 1.3 9.6 # 8 8 8 8 8 8 8 8 8 8 8 LOC/TESTS P VALUE 0.930.22 0.85 0.79 0.05* 0.03* 0.85 0.38 0.08+ 0.52 0.08+ LegendAbbreviations BARREN % = Barren Plants (Percent) DROP % = Dropped Ears(Percent) EHT INCH = Ear Height (Inches) FINAL = Final Stand MST % =Moisture (Percent) PHT INCH = Plant Height (Inches) RTL % = Root Lodging(Percent) SHED GDU = GDUs to Shed SILK GDU = GDUs to Silk STL % = StalkLodging (Percent) YLD BU/A = Yield (Bushels/Acre) Significance levelsare indicated as: +10 percent *5 percent

TABLE 9B COMPARISON OF WDAQ2 WITH FBLL BARREN DROP EHT MST PHT RTL SHEDSILK STL YLD INBRED % % INCH FINAL % INCH % GDU GDU % BU/A 6F905 0.8 0.438.2 57.5 22.8 75.8 0.2 1655.0 1661.6 3.5 68.1 F118 1.5 0.3 27.4 55.323.0 66.7 0.0 1544.8 1565.2 2.3 58.0 DIFF −0.7 0.1 10.8 2.3 −0.2 9.1 0.2110.2 96.5 1.2 10.1 # 17 14 16 16 13 16 14 15 15 15 16 LOC/TESTS P VALUE0.75 0.83 0.00** 0.12 1.00 0.00** 0.75 0.00** 0.00** 0.37 0.01** LegendAbbreviations BARREN % = Barren Plants (Percent) DROP % = Dropped Ears(Percent) EHT INCH = Ear Height (Inches) FINAL = Final Stand MST % =Moisture (Percent) PHT INCH = Plant Height (Inches) RTL % = Root Lodging(Percent) SHED GDU = GDUs to Shed SILK GDU = GDUs to Silk STL % = StalkLodging (Percent) YLD BU/A = Yield (Bushels/Acre) Significance levelsare indicated as: **1 percent

TABLE 9C COMPARISON OF WDAQ2 WITH WDAD1 BARREN DROP EHT MST PHT RTL SHEDSILK STL YLD INBRED % % INCH FINAL % INCH % GDU GDU % BU/A WDAQ2 0.8 0.438.2 57.5 22.5 75.8 0.2 1655.0 1661.6 3.5 68.1 WDAD1 2.2 0.4 25.3 58.615.5 62.2 0.0 1528.7 1545.8 0.8 51.5 DIFF −1.4 0.0 12.9 −1.0 6.9 13.60.2 126.3 115.9 2.7 16.6 # 17 14 16 16 13 16 14 15 15 15 16 LOC/TESTS PVALUE 0.53 0.96 0.00** 0.50 0.00** 0.00** 0.76 0.00** 0.00** 0.11 0.00**Legend Abbreviations BARREN % = Barren Plants (Percent) DROP % = DroppedEars (Percent) EHT INCH = Ear Height (Inches) FINAL = Final Stand MST %= Moisture (Percent) PHT INCH = Plant Height (Inches) RTL % = RootLodging (Percent) SHED GDU = GDUs to Shed SILK GDU = GDUs to Silk STL %= Stalk Lodging (Percent) YLD BU/A = Yield (Bushels/Acre) Significancelevels are indicated as: **1 percent

A. Origin and Breeding History

Inbred plant WDAQ2 was derived from the cross between inbred plantsdesignated B73HT (a public inbred) and a line selected from the hybrid3378 (a hybrid of Pioneer Hi-Bred International).

WDAQ2 shows uniformity and stability within the limits of environmentalinfluence for the traits described hereinafter in Table 10. WDAQ2 hasbeen self-pollinated and ear-rowed a sufficient number of generationswith careful attention paid to uniformity of plant type to ensurehomozygosity and phenotypic stability. No variant traits have beenobserved or are expected in WDAQ2.

Inbred corn plants can be reproduced by planting such inbred seeds,growing the resulting corn plants under self-pollinating orsib-pollinating conditions with adequate isolation using standardtechniques well known to an artisan skilled in the agricultural arts.Seeds can be harvested from such a plant using standard, well knownprocedures.

The origin and breeding history of inbred plant WDAQ2 can be summarizedas follows:

Summer 1985

-   -   Inbred line B73HT was crossed with a selection from the hybrid        3378 (nursery row 117/60, 117/77).

Winter 1985/86

-   -   A backcross was made using the 3378 selection as the recurrent        parent (nursery row 25/80, 25/81).

Summer 1986

-   -   BC₁S₁ ears were grown (nursery row numbers 20/50-43).

Winter 1986/87

-   -   BC₁S₂ ears were grown ear-to-row (nursery row number 551/90).

Summer 1987

-   -   BC₁S₃ ears were grown ear-to-row (nursery row number 40/30).

Winter 1987/88

-   -   BC₁S₄ ears were grown ear-to-row (nursery row number 91/154).

Summer 1988

-   -   BC₁S₅ ears were grown ear-to-row (nursery row number 222/38).        Ears harvested in the fall were given the designation WDAQ2.

B. Phenotypic Description

In accordance with another aspect of the present invention, there isprovided a corn plant having the functional and morphologicalcharacteristics of corn plant WDAQ2. A description of the functional andmorphological characteristics of corn plant WDAQ2 is presented in Table10.

TABLE 10 MORPHOLOGICAL TRAITS FOR THE WDAQ2 PHENOTYPE YEAR OF DATA:1992, 1993 CHARACTERISTIC VALUE* 1. Stalk Diameter (Width) cm. 2.6Anthocyanin BASAL WEAK Nodes With Brace Roots 1.6 Brace Root Color GREENInternode Direction STRAIGHT Internode Length cm. 16.7 2. Leaf AngleUPRIGHT Number 19.7 Color MEDIUM GREEN Length cm. 83.7 Width cm. 9.3Sheath Anthocyanin STRONG Sheath Pubescence HEAVY Marginal Waves FEWLongitudinal Creases FEW 3. Tassel Length cm. 50.5 Spike Length cm. 24.0Peduncle Length cm. 15.7 Attitude COMPACT Branch Angle UPRIGHT BranchNumber 7.6 Glume Color YELLOW Glume Band ABSENT 4. Ear Silk ColorGREEN-YELLOW Number Per Stalk 1.0 Position (Attitude) UPRIGHT Length cm.16.4 Shape SEMI-CONICAL Diameter cm. 42.3 Weight gm. 157.6 Shank Lengthcm. 9.8 Shank Internode Number 6.6 Husk Bract SHORT Husk Cover cm. 2.2Husk Opening OPEN Husk Color Fresh GREEN Husk Color Dry BUFF CobDiameter cm. 23.0 Cob Color WHITE Shelling Percent 85.5 5. Kernel RowNumber 17.7 Number Per Row 35.7 Row Direction CURVED Type DENT Cap ColorYELLOW Side Color DEEP YELLOW Length (Depth) mm. 11.8 Width mm. 7.3Thickness 4.1 Weight of 1000K gm. 260.8 Endosperm Type NORMAL EndospermColor YELLOW *These are typical values. Values may vary due toenvironment. Other values that are substantially equivalent are alsowithin the scope of the invention. Substantially equivalent refers toquantitative traits that when compared do not show statisticaldifferences of their means.VIII. Inbred Corn Plant WDDQ1

In accordance with another aspect of the present invention, there isprovided a novel inbred corn plant, designated WDDQ1. Inbred corn plantWDDQ1 is a yellow, dent corn inbred that can be compared to inbred cornplants 84BRQ4, B73HT, FBLL, and LH132. WDDQ1 differs significantly (atthe 5% level) from these inbred lines in several aspects (Table 11A,Table 11B, Table 11C, Table 11D).

TABLE 11A COMPARISON OF WDDQ1 WITH 84BRQ4 BARREN DROP EHT MST PHT RTLSHED SILK STL YLD INBRED % % INCH FINAL % INCH % GDU GDU % BU/A WDDQ10.6 0.1 25.7 58.8 22.8 68.8 0.1 1580.6 1620.5 0.3 56.9 84BRQ4 1.2 0.229.5 57.5 22.4 73.9 0.1 1569.1 1586.9 1.1 65.2 DIFF −0.6 −0.1 −3.8 1.30.5 −5.1 −0.1 11.5 33.6 −0.8 −8.3 # 11 16 16 16 14 16 16 16 16 16 16LOC/TESTS P VALUE 0.67 0.68 0.00** 0.27 0.72 0.00** 0.96 0.16 0.00**0.26 0.09+ Legend Abbreviations BARREN % = Barren Plants (Percent) DROP% = Dropped Ears (Percent) EHT INCH = Ear Height (Inches) FINAL = FinalStand MST % = Moisture (Percent) PHT INCH = Plant Height (Inches) RTL %= Root Lodging (Percent) SHED GDU = GDUs to Shed SILK GDU = GDUs to SilkSTL % = Stalk Lodging (Percent) YLD BU/A = Yield (Bushels/Acre)Significance levels are indicated as: +10 percent **1 percent

TABLE 11B COMPARISON OF WDDQ1 WITH B73HT BARREN DROP EHT MST PHT RTLSHED SILK STL YLD INBRED % % INCH FINAL % INCH % GDU GDU % BU/A WDDQ10.6 0.1 25.7 58.8 22.8 68.8 0.1 1580.6 1620.5 0.3 56.9 B73HT 0.8 0.336.7 59.6 21.9 79.9 0.5 1592.3 1599.6 2.0 72.5 DIFF −0.2 −0.2 −11.0 −0.80.9 −11.2 −0.5 −11.7 20.8 −1.7 −15.6 # 11 16 16 16 14 16 16 16 16 16 16LOC/TESTS P VALUE 0.89 0.56 0.00** 0.46 0.44 0.00** 0.66 0.18 0.03*0.03* 0.00** Legend Abbreviations BARREN % = Barren Plants (Percent)DROP % = Dropped Ears (Percent) EHT INCH = Ear Height (Inches) FINAL =Final Stand MST % = Moisture (Percent) PHT INCH = Plant Height (Inches)RTL % = Root Lodging (Percent) SHED GDU = GDUs to Shed SILK GDU = GDUsto Silk STL % = Stalk Lodging (Percent) YLD BU/A = Yield (Bushels/Acre)Significance levels are indicated as: *5 percent **1 percent

TABLE 11C COMPARISON OF WDDQ1 WITH FBLL BARREN DROP EHT MST PHT RTL SHEDSILK STL YLD INBRED % % INCH FINAL % INCH % GDU GDU % BU/A WDDQ1 1.3 0.024.1 58.8 22.4 68.3 0.1 1547.3 1591.6 0.0 59.6 FBLL 0.7 0.4 27.9 58.519.6 70.4 0.1 1441.6 1461.8 1.1 89.4 DIFF 0.7 −0.4 −3.8 0.3 2.8 −2.1 0.0105.6 129.9 −1.1 −29.8 # 3 8 8 8 8 8 8 8 8 8 8 LOC/TESTS P VALUE 0.720.23 0.00** 0.84 0.06+ 0.16 1.00 0.00** 0.00** 0.21 0.00** LegendAbbreviations BARREN % = Barren Plants (Percent) DROP % = Dropped Ears(Percent) EHT INCH = Ear Height (Inches) FINAL = Final Stand MST % =Moisture (Percent) PHT INCH = Plant Height (Inches) RTL % = Root Lodging(Percent) SHED GDU = GDUs to Shed SILK GDU = GDUs to Silk STL % = StalkLodging (Percent) YLD BU/A = Yield (Bushels/Acre) Significance levelsare indicated as: +10 percent **1 percent

TABLE 11D COMPARISON OF WDDQ1 WITH LH132 BARREN DROP EHT MST PHT RTLSHED SILK STL YLD INBRED % % INCH FINAL % INCH % GDU GDU % BU/A WDDQ10.6 0.1 25.7 58.8 22.8 68.8 0.1 1580.6 1620.5 0.3 56.9 LH132 1.4 0.025.9 59.2 17.5 68.1 0.0 1495.7 1497.8 1.5 74.1 DIFF −0.8 0.1 −0.3 −0.35.3 0.6 0.1 84.9 122.7 −1.2 −17.2 # 11 16 16 16 15 16 16 16 16 16 16LOC/TESTS P VALUE 0.58 0.90 0.79 0.60 0.00** 0.63 0.96 0.00** 0.00**0.11 0.00** Legend Abbreviations BARREN % = Barren Plants (Percent) DROP% = Dropped Ears (Percent) EHT INCH = Ear Height (Inches) FINAL = FinalStand MST % = Moisture (Percent) PHT INCH = Plant Height (Inches) RTL %= Root Lodging (Percent) SHED GDU = GDUs to Shed SILK GDU = GDUs to SilkSTL % = Stalk Lodging (Percent) YLD BU/A = Yield (Bushels/Acre)Significance levels are indicated as: **1 percent

A. Origin and Breeding History

Inbred plant WDDQ1 was derived from a cross between inbred plant LH132(a proprietary line of Holden's Foundation Seeds, Inc.) and a lineselected from 3378 (a hybrid developed by Pioneer Hi-BredInternational).

WDDQ1 shows uniformity and stability within the limits of environmentalinfluence for the traits described hereinafter in Table 12. WDDQ1 hasbeen self-pollinated and ear-rowed a sufficient number of generationswith careful attention paid to uniformity of plant type to ensurehomozygosity and phenotypic stability. No variant traits have beenobserved or are expected in WDDQ1.

Inbred corn plants can be reproduced by planting such inbred seeds,growing the resulting corn plants under self-pollinating orsib-pollinating conditions with adequate isolation using standardtechniques well known to an artisan skilled in the agricultural arts.Seeds can be harvested from such a plant using standard, well knownprocedures.

The origin and breeding history of inbred plant WDDQ1 can be summarizedas follows:

Summer 1987

-   -   The backcross of LH132 and a selection from the hybrid Pioneer        3378 was grown and plants were selfed. The recurrent parent in        the backcross was LH132. LH132 is a proprietary line of Holden's        Foundation Seeds, Inc.

Summer 1988

-   -   BC₁S₁ ears were grown (nursery rows 326-50).

Winter 1988-89

-   -   BC₁S₂ ears were grown (nursery rows 435-94).

Summer 1989

-   -   BC₁S₃ ears were grown (nursery rows 222-20).

Winter 1989-90

-   -   BC₁S₄ ears were grown (nursery rows 99-92).

Summer 1990

-   -   BC₁S₅ ears were grown. The seed was assigned the inbred code        WDDQ1 (nursery rows 223-44).

B. Phenotypic Description

In accordance with another aspect of the present invention, there isprovided a corn plant having the functional and morphologicalcharacteristics of corn plant WDDQ1. A description of the functional andmorphological characteristics of corn plant WDDQ1 is presented in Table12.

TABLE 12 MORPHOLOGICAL TRAITS FOR THE WDDQ1 PHENOTYPE YEAR OF DATA:1992, 1993 CHARACTERISTIC VALUE* 1. Stalk Diameter (Width) cm. 2.4Anthocyanin ABSENT Nodes With Brace Roots 2.3 Internode Length cm. 12.82. Leaf Number 19.8 Length cm. 77.0 Width cm. 9.6 3. Tassel Length cm.37.9 Spike Length cm. 24.4 Peduncle Length cm. 9.8 Attitude COMPACTBranch Angle INTERMEDIATE Branch Number 5.7 Anther Color TAN Glume ColorGREEN Glume Band ABSENT 4. Ear Silk Color GREEN-YELLOW Number Per Stalk1.0 Position (Attitude) UPRIGHT Length cm. 16.7 Shape SEMI-CONICALDiameter cm. 37.9 Weight gm. 124.9 Shank Length cm. 9.5 Shank InternodeNumber 7.8 Husk Bract SHORT Husk Cover cm. 4.1 Husk Color Fresh GREENHusk Color Dry BUFF Cob Diameter cm. 21.2 Cob Color RED Cob StrengthWEAK Shelling Percent 84.8 5. Kernel Row Number 14.5 Number Per Row 33.3Row Direction CURVED Cap Color YELLOW Side Color ORANGE Length (Depth)mm. 10.4 Width mm. 7.3 Thickness 4.5 Weight of 1000K gm. 256.6 EndospermType NORMAL Endosperm Color YELLOW *These are typical values. Values mayvary due to environment. Other values that are substantially equivalentare also within the scope of the invention. Substantially equivalentrefers to quantitative traits that when compared do not show statisticaldifferences of their means.IX. Inbred Corn Plant 85DGD1

In accordance with another aspect of the present invention, there isprovided a novel inbred corn plant, designated 85DGD1. Inbred corn plant85DGD1 is a yellow, dent corn inbred that can be compared to inbred cornplants B73HT, FBLL, LH119, and LH132. B73HT is a publicly availableinbred, LH119 and LH132 are inbreds developed by Holden's FoundationSeeds, Inc., and FBLL is a proprietary inbred of DEKALB GeneticsCorporation. 85DGD1 differs significantly (at the 5% level) from theseinbred lines in several aspects (Table 13A, Table 13B, Table 13C, Table13D).

TABLE 13A COMPARISON OF 85DGD1 WITH B73HT BARREN DROP EHT MST PHT RTLSHED SILK STL YLD INBRED % % INCH FINAL % INCH % GDU GDU % BU/A 85DGD11.6 0.1 36.3 59.2 17.2 76.7 0.0 1558.4 1560.4 1.9 66.8 B73HT 1.2 0.639.0 58.3 23.0 80.8 0.5 1637.9 1639.8 2.1 59.5 DIFF 0.4 −0.6 −2.8 0.9−5.8 −4.1 −0.5 −79.5 −79.4 −0.2 7.3 # 16 16 16 16 13 16 16 16 16 16 16LOC/TESTS P VALUE 0.88 0.11 0.00** 0.54 0.00** 0.00** 0.60 0.00** 0.00**0.89 0.04* Legend Abbreviations BARREN % = Barren Plants (Percent) DROP% = Dropped Ears (Percent) EHT INCH = Ear Height (Inches) FINAL = FinalStand MST % = Moisture (Percent) PHT INCH = Plant Height (Inches) RTL %= Root Lodging (Percent) SHED GDU = GDUs to Shed SILK GDU = GDUs to SilkSTL % = Stalk Lodging (Percent) YLD BU/A = Yield (Bushels/Acre)Significance levels are indicated as: *5 percent **1 percent

TABLE 13B COMPARISON OF 85DGD1 WITH FBLL BARREN DROP EHT MST PHT RTLSHED SILK STL YLD INBRED % % INCH FINAL % INCH % GDU GDU % BU/A 85DGD11.5 0.2 36.7 59.0 26.3 78.1 0.2 1534.2 1534.3 1.1 73.0 FBLL 1.7 0.1 29.456.4 28.2 71.6 0.2 1481.7 1504.6 0.7 69.6 DIFF −0.2 0.1 7.2 2.6 −1.9 6.50.0 52.6 29.7 0.4 3.4 # 17 24 25 25 23 25 24 25 24 25 25 LOC/TESTS PVALUE 0.60 0.61 0.00** 0.02* 0.04* 0.00** 0.58 0.00** 0.00** 0.71 0.27Legend Abbreviations BARREN % = Barren Plants (Percent) DROP % = DroppedEars (Percent) EHT INCH = Ear Height (Inches) FINAL = Final Stand MST %= Moisture (Percent) PHT INCH = Plant Height (Inches) RTL % = RootLodging (Percent) SHED GDU = GDUs to Shed SILK GDU = GDUs to Silk STL %= Stalk Lodging (Percent) YLD BU/A = Yield (Bushels/Acre) Significancelevels are indicated as: *5 percent **1 percent

TABLE 13C COMPARISON OF 85DGD1 WITH LH119 BARREN DROP EHT MST PHT RTLSHED SILK STL YLD INBRED % % INCH FINAL % INCH % GDU GDU % BU/A 85DGD11.4 0.1 36.4 58.8 21.7 77.4 0.0 1546.0 1547.1 1.3 70.9 LH119 3.5 0.126.5 58.9 21.1 68.3 0.0 1496.4 1520.2 1.3 68.2 DIFF −2.2 0.0 9.9 −0.10.6 9.1 0.0 49.6 26.9 0.1 2.7 # 21 26 26 26 24 26 26 26 26 26 26LOC/TESTS P VALUE 0.26 0.78 0.00** 0.75 0.31 0.00** 0.97 0.00** 0.00**0.95 0.41 Legend Abbreviations BARREN % = Barren Plants (Percent) DROP %= Dropped Ears (Percent) EHT INCH = Ear Height (Inches) FINAL = FinalStand MST % = Moisture (Percent) PHT INCH = Plant Height (Inches) RTL %= Root Lodging (Percent) SHED GDU = GDUs to Shed SILK GDU = GDUs to SilkSTL % = Stalk Lodging (Percent) YLD BU/A = Yield (Bushels/Acre)Significance levels are indicated as: **1 percent

TABLE 13D COMPARISON OF 85DGD1 WITH LH132 BARREN DROP EHT MST PHT RTLSHED SILK STL YLD INBRED % % INCH FINAL % INCH % GDU GDU % BU/A 85DGD11.4 0.1 36.4 58.8 21.7 77.4 0.0 1546.0 1547.1 1.3 70.9 LH132 2.4 0.027.2 58.3 20.7 68.1 0.0 1507.6 1519.5 0.9 68.9 DIFF −1.0 0.0 9.2 0.5 1.09.3 −0.0 38.4 27.6 0.4 2.0 # 21 26 26 26 24 26 26 26 26 26 26 LOC/TESTSP VALUE 0.49 0.72 0.00** 0.81 0.15 0.00** 1.00 0.00** 0.00** 0.71 0.48Legend Abbreviations BARREN % = Barren Plants (Percent) DROP % = DroppedEars (Percent) EHT INCH = Ear Height (Inches) FINAL = Final Stand MST %= Moisture (Percent) PHT INCH = Plant Height (Inches) RTL % = RootLodging (Percent) SHED GDU = GDUs to Shed SILK GDU = GDUs to Silk STL %= Stalk Lodging (Percent) YLD BU/A = Yield (Bushels/Acre) Significancelevels are indicated as: **1 percent

A. Origin and Breeding History

Inbred plant 85DGD1 was derived from the cross of two inbred plantsdesignated LH119 (an inbred developed by Holden's Foundation Seeds,Inc.) and PB80 (a proprietary inbred developed by DEKALB GeneticsCorporation).

85DGD1 shows uniformity and stability within the limits of environmentalinfluence for the traits described hereinafter in Table 14. 85DGD1 hasbeen self-pollinated and ear-rowed a sufficient number of generationswith careful attention paid to uniformity of plant type to ensurehomozygosity and phenotypic stability. No variant traits have beenobserved or are expected in 85DGD1.

A deposit of 2500 seeds of the plant designated 85DGD1 has been madewith the American Type Culture Collection (ATCC), 10801 UniversityBlvd., Manassas, Va. on Aug. 18, 2004. Those deposited seeds have beenassigned Accession No. PTA-6160. This deposit was made in accordancewith the terms and provisions of the Budapest Treaty relating to depositof microorganisms and is made for a term of at least thirty (30) yearsand at least five (05) years after the most recent request for thefurnishing of a sample of the deposit was received by the depository, orfor the effective term of the patent, whichever is longer, and will bereplaced if it becomes non-viable during that period.

Inbred corn plants can be reproduced by planting such inbred seeds,growing the resulting corn plants under self-pollinating orsib-pollinating conditions with adequate isolation using standardtechniques well known to an artisan skilled in the agricultural arts.Seeds can be harvested from such a plant using standard, well knownprocedures.

The origin and breeding history of inbred plant 85DGD1 can be summarizedas follows:

Summer 1985

-   -   The cross LH119×PB80 was made (nursery row numbers SJO        99/15×98/9).

Summer 1986

-   -   S₀ generation grown and plants were backcrossed to PB80 (nursery        row ICB-2-40-119). All seed was bulked.

Summer 1987

-   -   Bulked seed of PB80×LH119 grown and plants were selfed (nursery        rows 320/1-13)

Winter 1987

-   -   BC₁S₁ generation grown and self-pollinated (nursery row numbers        651/74-100).

Summer 1988

-   -   BC₁S₂ generation grown and self-pollinated (nursery rows        178/1-34).

Winter 1988

-   -   BC₁S₃ generation grown and self-pollinated (nursery rows        47/135-144).

Summer 1989

-   -   BC₁S₄ generation grown and self-pollinated (nursery rows        324/55-44).

Winter 1989

-   -   BC₁S₅ generation grown and self-pollinated (nursery rows        40/142-138).

Summer 1990

-   -   BC₁S₆ generation grown, self-pollinated, and seed was bulked.

B. Phenotypic Description

In accordance with another aspect of the present invention, there isprovided a corn plant having the functional and morphologicalcharacteristics of corn plant 85DGD1. A description of the functionaland morphological characteristics of corn plant 85DGD1 is presented inTable 14.

TABLE 14 MORPHOLOGICAL TRAITS FOR THE 85DGD1 PHENOTYPE YEAR OF DATA:1992, 1993 CHARACTERISTIC VALUE* 1. Stalk Diameter (Width) cm. 2.3 NodesWith Brace Roots 2.0 Internode Length cm. 15.0 2. Leaf Number 19.9 ColorMEDIUM GREEN Length cm. 77.6 Width cm. 9.2 Marginal Waves FEW 3. TasselLength cm. 36.3 Spike Length cm. 21.3 Peduncle Length cm. 11.8 AttitudeCOMPACT Branch Angle UPRIGHT Branch Number 6.8 Glume Color GREEN GlumeBand ABSENT 4. Ear Silk Color GREEN-YELLOW Number Per Stalk 1.3 Position(Attitude) UPRIGHT Length cm. 13.4 Shape SEMI-CONICAL Diameter cm. 40.6Weight gm. 106.3 Shank Length cm. 12.4 Shank Internode Number 6.7 HuskBract SHORT Husk Cover cm. 7.9 Husk Color Fresh GREEN Husk Color DryBUFF Cob Diameter cm. 24.4 Cob Strength STRONG Shelling Percent 82.2 5.Kernel Row Number 15.6 Number Per Row 27.0 Row Direction CURVED TypeDENT Side Color DEEP YELLOW Length (Depth) mm. 11.0 Width mm. 7.4Thickness 4.3 Weight of 1000K gm. 258.3 Endosperm Type NORMAL EndospermColor YELLOW *These are typical values. Values may vary due toenvironment. Other values that are substantially equivalent are alsowithin the scope of the invention. Substantially equivalent refers toquantitative traits that when compared do not show statisticaldifferences of their means.X. Inbred Corn Plant PHEI4

In accordance with another aspect of the present invention, there isprovided a novel inbred corn plant, designated PHEI4. Inbred corn plantPHEI4 is a yellow, dent corn inbred that can be compared to inbred cornplants MBWZ and MBZA, both proprietary inbreds of DEKALB GeneticsCorporation. PHEI4 differs significantly (at the 5% level) from theseinbred lines in several aspects (Table 15A, Table 15B).

TABLE 15A COMPARISON OF PHEI4 WITH MBWZ BARREN DROP EHT MST PHT RTL SHEDSILK STL YLD INBRED % % INCH FINAL % INCH % GDU GDU % BU/A PHEI4 0.8 0.131.9 57.9 19.1 67.9 0.2 1536.8 1550.6 2.2 64.7 MBWZ 1.7 0.4 31.5 57.123.8 75.5 0.4 1597.4 1634.5 4.6 66.7 DIFF −0.9 −0.2 0.3 0.8 −4.7 −7.6−0.2 −60.6 −83.9 −2.4 −2.0 # 22 22 23 24 20 23 22 24 24 23 24 LOC/TESTSP VALUE 0.59 0.43 0.72 0.84 0.00** 0.00** 0.90 0.00** 0.00** 0.02* 0.65Legend Abbreviations BARREN % = Barren Plants (Percent) DROP % = DroppedEars (Percent) EHT INCH = Ear Height (Inches) FINAL = Final Stand MST %= Moisture (Percent) PHT INCH = Plant Height (Inches) RTL % = RootLodging (Percent) SHED GDU = GDUs to Shed SILK GDU = GDUs to Silk STL %= Stalk Lodging (Percent) YLD BU/A = Yield (Bushels/Acre) Significancelevels are indicated as: *5 percent **1 percent

TABLE 15B COMPARISON OF PHEI4 WITH MBZA BARREN DROP EHT MST PHT RTL SHEDSILK STL YLD INBRED % % INCH FINAL % INCH % GDU GDU % BU/A PHEI4 0.8 0.131.9 57.9 19.1 67.9 0.2 1536.8 1550.6 2.1 64.7 MBZA 1.9 0.5 24.5 57.419.2 56.3 0.2 1595.0 1604.5 1.7 65.1 DIFF −1.0 −0.4 7.3 0.5 −0.1 11.6−0.0 −58.2 −54.0 0.5 −0.4 # 22 22 23 24 21 23 22 24 24 22 24 LOC/TESTS PVALUE 0.55 0.27 0.00** 0.84 0.93 0.00** 0.94 0.00** 0.00** 0.81 0.88Legend Abbreviations BARREN % = Barren Plants (Percent) DROP % = DroppedEars (Percent) EHT INCH = Ear Height (Inches) FINAL = Final Stand MST %= Moisture (Percent) PHT INCH = Plant Height (Inches) RTL % = RootLodging (Percent) SHED GDU = GDUs to Shed SILK GDU = GDUs to Silk STL %= Stalk Lodging (Percent) YLD BU/A = Yield (Bushels/Acre) Significancelevels are indicated as: **1 percent

A. Origin and Breeding History

Inbred plant PHEI4 was derived from the cross of two inbred plantsdesignated HBA1 and IBO14, both proprietary inbreds of DEKALB GeneticsCorporation, in the summer of 1983.

PHEI4 shows uniformity and stability within the limits of environmentalinfluence for the traits described hereinafter in Table 16. PHEI4 hasbeen self-pollinated and ear-rowed a sufficient number of generationswith careful attention paid to uniformity of plant type to ensurehomozygosity and phenotypic stability. No variant traits have beenobserved or are expected in PHEI4.

Inbred corn plants can be reproduced by planting such inbred seeds,growing the resulting corn plants under self-pollinating orsib-pollinating conditions with adequate isolation using standardtechniques well known to an artisan skilled in the agricultural arts.Seeds can be harvested from such a plant using standard, well knownprocedures.

The origin and breeding history of inbred plant PHEI4 can be summarizedas follows:

Summer 1983

-   -   The cross HBA1. IBO14 was made.

Winter 1983/84

-   -   S₀ seed was grown and self-pollinated.

Summer 1984

-   -   S₁ seed was grown and self-pollinated.

Winter 1984/85

-   -   S₂ ears were grown on an ear-to-row basis (nursery row 436/84).

Summer 1985

-   -   S₃ ears were grown on an ear-to-row basis (nursery rows        47/94,95).

Winter 1985/86

-   -   S₄ ears were grown on an ear-to-row basis (nursery rows        87/61-63).

Summer 1986

-   -   S₅ ears were grown on an ear-to-row basis (nursery rows        141/14-17).

Summer 1987

-   -   S₆ ears were grown on an ear-to-row basis (nursery rows        602/63,64).

Winter 1987/88

-   -   S₇ ears were grown on an ear-to-row basis (nursery row 82-173)        and selected ears were bulked at harvest.

Summer 1988

-   -   S₈ generation was grown (nursery row 501/36) and selected ears        bulked.

Winter 1988/89

-   -   S₉ generation was grown (nursery rows 112/107,108) and selected        ears were bulked. The bulked seed was given the designation        PHEI4 after harvest.

B. Phenotypic Description

In accordance with another aspect of the present invention, there isprovided a corn plant having the functional and morphologicalcharacteristics of corn plant PHEI4. A description of the functional andmorphological characteristics of corn plant PHEI4 is presented in Table16.

TABLE 16 MORPHOLOGICAL TRAITS FOR THE PHEI4 PHENOTYPE YEAR OF DATA:1992, 1993 CHARACTERISTIC VALUE* 1. Stalk Diameter (Width) cm. 2.2 NodesWith Brace Roots 1.6 Brace Root Color GREEN Internode Direction STRAIGHTInternode Length cm. 13.9 2. Leaf Number 19.3 Length cm. 79.0 Width cm.8.8 Sheath Pubescence LIGHT Marginal Waves FEW Longitudinal Creases FEW3. Tassel Length cm. 30.6 Spike Length cm. 18.6 Peduncle Length cm. 5.1Attitude COMPACT Branch Angle UPRIGHT Branch Number 10.5 Glume ColorGREEN Glume Band ABSENT 4. Ear Number Per Stalk 1.6 Position (Attitude)UPRIGHT Length cm. 15.8 Shape SEMI-CONICAL Diameter cm. 39.6 Weight gm.97.5 Shank Length cm. 9.8 Shank Internode Number 7.3 Husk Bract SHORTHusk Cover cm. 3.7 Husk Color Fresh GREEN Husk Color Dry BUFF CobDiameter cm. 25.3 Cob Strength WEAK Shelling Percent 81.9 5. Kernel RowNumber 13.9 Number Per Row 26.5 Row Direction CURVED Type DENT Cap ColorYELLOW Length (Depth) mm. 10.0 Width mm. 8.6 Thickness 4.7 Weight of1000K gm. 282.4 Endosperm Type NORMAL Endosperm Color YELLOW *These aretypical values. Values may vary due to environment. Other values thatare substantially equivalent are also within the scope of the invention.Substantially equivalent refers to quantitative traits that whencompared do not show statistical differences of their means.XI. Inbred Corn Plant 01ASB1

In accordance with another aspect of the present invention, there isprovided a novel inbred corn plant, designated 01ASB1. Inbred corn plant01ASB1 is a yellow, dent corn inbred that can be compared to inbred cornplants 2FACC, 4676A, 78010, and FBAB, all of which are proprietaryinbreds developed by DEKALB Genetics Corporation. 01ASB1 differssignificantly (at the 5% level) from these inbred lines in severalaspects (Table 17A, Table 17B, Table 17C, Table 17D).

TABLE 17A COMPARISON OF 01ASB1 WITH 2FACC BARREN DROP EHT MST PHT RTLSHED SILK STL YLD INBRED % % INCH FINAL % INCH % GDU GDU % BU/A 01ASB10.7 0.9 31.4 58.1 17.2 66.3 −0.2 1530.0 1518.8 11.3 44.9 2FACC 0.2 0.428.0 57.4 20.3 64.4 4.5 1508.9 1497.2 2.3 73.9 DIFF 0.5 0.6 3.5 0.7 −3.11.9 −4.7 21.1 21.5 9.0 −28.9 # 21 15 18 18 15 18 15 18 18 17 18LOC/TESTS P VALUE 0.68 0.17 0.00** 0.58 0.00** 0.15 0.25 0.02* 0.03*0.01** 0.00** Legend Abbreviations BARREN % = Barren Plants (Percent)DROP % = Dropped Ears (Percent) EHT INCH = Ear Height (Inches) FINAL =Final Stand MST % = Moisture (Percent) PHT INCH = Plant Height (Inches)RTL % = Root Lodging (Percent) SHED GDU = GDUs to Shed SILK GDU = GDUsto Silk STL % = Stalk Lodging (Percent) YLD BU/A = Yield (Bushels/Acre)Significance levels are indicated as: *5 percent **1 percent

TABLE 17B COMPARISON OF 01ASB1 WITH 4676A BARREN DROP EHT MST PHT RTLSHED SILK STL YLD INBRED % % INCH FINAL % INCH % GDU GDU % BU/A 01ASB10.4 1.4 33.8 60.2 19.1 69.1 −1.1 1520.0 1508.7 18.4 50.5 4676A 0.1 2.530.6 59.4 21.7 67.8 11.5 1449.8 1435.2 8.9 61.7 DIFF 0.3 −1.1 3.2 0.8−2.6 1.3 −12.6 70.2 73.5 9.5 −11.2 # 6 4 5 5 5 5 4 5 5 5 5 LOC/TESTS PVALUE 0.84 0.34 0.12 0.71 0.07+ 0.59 0.16 0.00** 0.00** 0.19 0.13 LegendAbbreviations BARREN % = Barren Plants (Percent) DROP % = Dropped Ears(Percent) EHT INCH = Ear Height (Inches) FINAL = Final Stand MST % =Moisture (Percent) PHT INCH = Plant Height (Inches) RTL % = Root Lodging(Percent) SHED GDU = GDUs to Shed SILK GDU = GDUs to Silk STL % = StalkLodging (Percent) YLD BU/A = Yield (Bushels/Acre) Significance levelsare indicated as: +10 percent **1 percent

TABLE 17C COMPARISON OF 01ASB1 WITH 78010 BARREN DROP EHT MST PHT RTLSHED SILK STL YLD INBRED % % INCH FINAL % INCH % GDU GDU % BU/A 01ASB10.5 0.7 32.6 59.3 19.1 69.3 −0.2 1483.7 1474.3 9.3 54.2 78010 0.9 0.728.8 59.1 22.9 58.1 1.7 1482.1 1503.9 3.0 63.8 DIFF −0.4 −0.0 3.8 0.2−3.7 11.3 −1.9 1.6 −29.6 6.3 −9.6 # 16 15 17 17 16 17 15 17 17 17 17LOC/TESTS P VALUE 0.41 0.38 0.00** 0.90 0.00** 0.00** 0.68 0.60 0.00**0.15 0.01** Legend Abbreviations BARREN % = Barren Plants (Percent) DROP% = Dropped Ears (Percent) EHT INCH = Ear Height (Inches) FINAL = FinalStand MST % = Moisture (Percent) PHT INCH = Plant Height (Inches) RTL %= Root Lodging (Percent) SHED GDU = GDUs to Shed SILK GDU = GDUs to SilkSTL % = Stalk Lodging (Percent) YLD BU/A = Yield (Bushels/Acre)Significance levels are indicated as: **1 percent

TABLE 17D COMPARISON OF 01ASB1 WITH FBAB BARREN DROP EHT MST PHT RTLSHED SILK STL YLD INBRED % % INCH FINAL % INCH % GDU GDU % BU/A 01ASB10.7 0.8 31.7 58.3 18.2 67.7 −0.1 1503.0 1493.2 8.8 49.1 FBAB 2.9 0.528.5 58.8 16.8 72.4 1.8 1453.2 1470.1 17.8 38.0 DIFF −2.1 0.3 3.2 −0.51.4 −4.7 −1.9 49.8 23.1 −9.0 11.0 # 25 23 25 25 17 25 22 25 25 25 25LOC/TESTS P VALUE 0.04* 0.37 0.00** 0.64 0.07+ 0.00** 0.63 0.00** 0.00**0.00** 0.00** Legend Abbreviations BARREN % = Barren Plants (Percent)DROP % = Dropped Ears (Percent) EHT INCH = Ear Height (Inches) FINAL =Final Stand MST % = Moisture (Percent) PHT INCH = Plant Height (Inches)RTL % = Root Lodging (Percent) SHED GDU = GDUs to Shed SILK GDU = GDUsto Silk STL % = Stalk Lodging (Percent) YLD BU/A = Yield (Bushels/Acre)Significance levels are indicated as: +10 percent *5 percent **1 percent

A. Origin and Breeding History

Inbred plant 01ASB1 was derived from the cross of two inbred plantsdesignated 4676A and NL001, both proprietary inbreds of DEKALB GeneticsCorporation, in the summer of 1985.

01ASB1 shows uniformity and stability within the limits of environmentalinfluence for the traits described hereinafter in Table 18. 01ASB1 hasbeen self-pollinated and ear-rowed a sufficient number of generationswith careful attention paid to uniformity of plant type to ensurehomozygosity and phenotypic stability. No variant traits have beenobserved or are expected in 01ASB1.

Inbred corn plants can be reproduced by planting such inbred seeds,growing the resulting corn plants under self-pollinating orsib-pollinating conditions with adequate isolation using standardtechniques well known to an artisan skilled in the agricultural arts.Seeds can be harvested from such a plant using standard, well knownprocedures.

The origin and breeding history of inbred plant 01ASB1 can be summarizedas follows:

Summer 1985

-   -   The cross 4676A×NL001 was made (nursery book row numbers 18049        and 18143, respectively). Both parents are proprietary lines of        DEKALB Genetics Corporation.

Winter 1985

-   -   S₀ seed was grown and plants were selfed (nursery row number        30137).

Summer 1986

-   -   S₁ seed was grown and plants were selfed (nursery rows 51 to        100). S₂ ears selected.

Winter 1986

-   -   S₂ ears were grown ear-to-row and plants were selfed (nursery        book row numbers 738-29 to 738-69).

Summer 1987

-   -   S₃ ears were grown ear-to-row and plants were selfed (nursery        rows 20-74 to 20-72).

Summer 1988

-   -   S₄ ears were grown ear-to-row and plants were selfed (nursery        rows 32-34 to 32-31).

Winter 1988

-   -   S₅ ears were grown ear-to-row and plants were selfed (nursery        rows 102-95 to 102-93).

Summer 1989

-   -   S₆ ears were grown ear-to-row and plants were selfed (nursery        rows 204-24 to 204-22). Ears from one S₆ row were selected and        given the designation 01ASB1.

B. Phenotypic Description

In accordance with another aspect of the present invention, there isprovided a corn plant having the functional and morphologicalcharacteristics of corn plant 01ASB1. A description of the functionaland morphological characteristics of corn plant 01ASB1 is presented inTable 18.

TABLE 18 MORPHOLOGICAL TRAITS FOR THE 01ASB1 PHENOTYPE YEAR OF DATA:1992, 1993 CHARACTERISTIC VALUE* 1. Stalk Diameter (Width) cm. 2.1 NodesWith Brace Roots 1.1 Brace Root Color RED Internode Direction STRAIGHTInternode Length cm. 11.2 2. Leaf Number 20.3 Color MEDIUM GREEN Lengthcm. 73.9 Width cm. 6.6 Sheath Anthocyanin WEAK Sheath Pubescence LIGHTLongitudinal Creases FEW 3. Tassel Length cm. 32.1 Spike Length cm. 22.5Peduncle Length cm. 6.6 Attitude COMPACT Branch Angle INTERMEDIATEBranch Number 4.5 Anther Color RED Glume Color GREEN Glume Band ABSENT4. Ear Number Per Stalk 1.0 Position (Attitude) UPRIGHT Length cm. 12.1Diameter cm. 33.5 Weight gm. 68.1 Shank Length cm. 11.9 Shank InternodeNumber 4.5 Husk Bract SHORT Husk Cover cm. 8.7 Husk Opening INTERMEDIATEHusk Color Fresh GREEN Husk Color Dry BUFF Cob Diameter cm. 17.7 CobColor RED Cob Strength STRONG Shelling Percent 83.5 5. Kernel Row Number11.9 Number Per Row 25.1 Row Direction CURVED Type DENT Cap Color YELLOWLength (Depth) mm. 9.5 Width mm. 8.0 Thickness 3.7 Weight of 1000K gm.209.0 Endosperm Type NORMAL Endosperm Color YELLOW *These are typicalvalues. Values may vary due to environment. Other values that aresubstantially equivalent are also within the scope of the invention.Substantially equivalent refers to quantitative traits that whencompared do not show statistical differences of their means.XII. Inbred Corn Plant 01IBH2

In accordance with another aspect of the present invention, there isprovided a novel inbred corn plant, designated 01IBH2. Inbred corn plant01IBH2 is a yellow, dent corn inbred that can be compared to inbred cornplants 3IIH6, 78551S, and FBLL, all of which are proprietary inbredsdeveloped by DEKALB Genetics Corporation. 01IBH2 differs significantly(at the 5% level) from these inbred lines in several aspects (Table 19A,Table 19B, Table 19C).

TABLE 19A COMPARISON OF 01IBH2 WITH 3IIH6 BARREN DROP EHT MST PHT RTLSHED SILK STL YLD INBRED % % INCH FINAL % INCH % GDU GDU % BU/A 01IBH20.6 0.3 27.6 58.1 23.1 64.9 2.9 1496.8 1515.1 1.4 71.1 3IIH6 1.3 0.427.9 58.0 17.8 64.3 0.5 1462.5 1484.7 5.1 60.4 DIFF −0.6 −0.1 −0.4 0.15.2 0.6 2.4 34.3 30.4 −3.7 10.7 # 25 27 28 28 25 28 27 28 28 28 28LOC/TESTS P VALUE 0.67 0.70 0.46 0.56 0.00** 0.68 0.83 0.00** 0.00**0.14 0.00** Legend Abbreviations BARREN % = Barren Plants (Percent) DROP% = Dropped Ears (Percent) EHT INCH = Ear Height (Inches) FINAL = FinalStand MST % = Moisture (Percent) PHT INCH = Plant Height (Inches) RTL %= Root Lodging (Percent) SHED GDU = GDUs to Shed SILK GDU = GDUs to SilkSTL % = Stalk Lodging (Percent) YLD BU/A = Yield (Bushels/Acre)Significance levels are indicated as: **1 percent

TABLE 19B COMPARISON OF 01IBH2 WITH 78551S BARREN DROP EHT MST PHT RTLSHED SILK STL YLD INBRED % % INCH FINAL % INCH % GDU GDU % BU/A 01IBH20.7 0.3 27.4 58.1 23.1 64.9 1.7 1492.3 1511.2 1.6 72.4 78551S 2.7 0.322.7 52.7 19.4 65.3 0.2 1436.5 1495.5 1.9 61.4 DIFF −2.0 0.0 4.7 5.4 3.8−0.3 1.5 55.8 15.7 −0.3 10.9 # 19 22 23 23 19 23 22 23 23 23 22LOC/TESTS P VALUE 0.12 0.89 0.00** 0.00** 0.00** 0.75 0.86 0.00** 0.01**0.91 0.00** Legend Abbreviations BARREN % = Barren Plants (Percent) DROP% = Dropped Ears (Percent) EHT INCH = Ear Height (Inches) FINAL = FinalStand MST % = Moisture (Percent) PHT INCH = Plant Height (Inches) RTL %= Root Lodging (Percent) SHED GDU = GDUs to Shed SILK GDU = GDUs to SilkSTL % = Stalk Lodging (Percent) YLD BU/A = Yield (Bushels/Acre)Significance levels are indicated as: **1 percent

TABLE 19C COMPARISON OF 01IBH2 WITH FBLL BARREN DROP EHT MST PHT RTLSHED SILK STL YLD INBRED % % INCH FINAL % INCH % GDU GDU % BU/A 01IBH20.6 0.3 27.6 58.1 23.1 64.9 2.9 1496.8 1515.1 1.4 71.1 FBLL 1.0 0.4 30.058.0 27.2 70.0 −0.2 1506.7 1531.9 2.8 69.8 DIFF −0.4 −0.0 −2.5 0.1 −4.1−5.1 3.1 −9.9 −16.9 −1.4 1.4 # 25 27 28 28 26 28 27 28 28 28 28LOC/TESTS P VALUE 0.55 0.84 0.00** 0.75 0.00** 0.00** 0.45 0.15 0.03*0.58 0.86 Legend Abbreviations BARREN % = Barren Plants (Percent) DROP %= Dropped Ears (Percent) EHT INCH = Ear Height (Inches) FINAL = FinalStand MST % = Moisture (Percent) PHT INCH = Plant Height (Inches) RTL %= Root Lodging (Percent) SHED GDU = GDUs to Shed SILK GDU = GDUs to SilkSTL % = Stalk Lodging (Percent) YLD BU/A = Yield (Bushels/Acre)Significance levels are indicated as: *5 percent **1 percent

A. Origin and Breeding History

Inbred plant 01IBH2 was derived from the hybrid Pioneer 3737. Pioneer3737 is publicly available and sold by Pioneer Hi-Bred International.

01IBH2 shows uniformity and stability within the limits of environmentalinfluence for the traits described hereinafter in Table 20. 01IBH2 hasbeen self-pollinated and ear-rowed a sufficient number of generationswith careful attention paid to uniformity of plant type to ensurehomozygosity and phenotypic stability. No variant traits have beenobserved or are expected in 01IBH2.

Inbred corn plants can be reproduced by planting such inbred seeds,growing the resulting corn plants under self-pollinating orsib-pollinating conditions with adequate isolation using standardtechniques well known to an artisan skilled in the agricultural arts.Seeds can be harvested from such a plant using standard, well knownprocedures.

The origin and breeding history of inbred plant 01IBH2 can be summarizedas follows:

Summer 1984

-   -   The hybrid Pioneer 3737 was self-pollinated to produce S₁ seed        (nursery row number 84:3848).

Summer 1985

-   -   S₁ seed was grown and selfed (nursery row numbers 3161 to 3170).

Summer 1986

-   -   S₂ ears were grown ear-to-row and plants were selfed (nursery        rows 6016 to 6025).

Summer 1987

-   -   S₃ ears were grown ear-to-row and plants were selfed (nursery        book row numbers 52-72 to 52-66).

Winter 1987

-   -   S₄ ears were grown ear-to-row and plants were selfed (nursery        rows 42-149 to 42-147).

Summer 1988

-   -   S₅ ears were grown ear-to-row and plants were selfed (nursery        rows 120-5 to 120-3).

Summer 1989

-   -   S₆ ears were grown ear-to-row and plants were selfed (nursery        rows 216-29 to 216-25). Ears from one row were selected and        given the designation 01IBH2.

B. Phenotypic Description

In accordance with another aspect of the present invention, there isprovided a corn plant having the functional and morphologicalcharacteristics of corn plant 01IBH2. A description of the functionaland morphological characteristics of corn plant 01IBH2 is presented inTable 20.

TABLE 20 MORPHOLOGICAL TRAITS FOR THE 01IBH2 PHENOTYPE YEAR OF DATA:1992, 1993 CHARACTERISTIC VALUE* 1. Stalk Diameter (Width) cm. 2.1Anthocyanin ABSENT Nodes With Brace Roots 1.1 Brace Root Color GREENInternode Direction STRAIGHT Internode Length cm. 14.4 2. Leaf AngleUPRIGHT Number 17.3 Color MEDIUM GREEN Length cm. 68.2 Width cm. 8.9Sheath Anthocyanin ABSENT Sheath Pubescence HEAVY Marginal Waves FEWLongitudinal Creases ABSENT 3. Tassel Length cm. 33.6 Spike Length cm.23.1 Peduncle Length cm. 8.2 Branch Number 7.8 Anther Color GREEN-YELLOWGlume Color GREEN Glume Band ABSENT 4. Ear Number Per Stalk 1.0 Lengthcm. 14.6 Shape SEMI-CONICAL Diameter cm. 40.4 Weight gm. 103.2 ShankLength cm. 10.1 Shank Internode Number 6.9 Husk Bract SHORT Husk Covercm. 4.4 Husk Color Fresh GREEN Husk Color Dry BUFF Cob Diameter cm. 22.9Cob Color RED Cob Strength WEAK Shelling Percent 89.0 5. Kernel RowNumber 16.3 Number Per Row 29.3 Row Direction CURVED Type DENT Cap ColorYELLOW Side Color ORANGE Length (Depth) mm. 10.9 Width mm. 7.4 Thickness4.4 Weight of 1000K gm. 233.0 Endosperm Type NORMAL Endosperm ColorYELLOW *These are typical values. Values may vary due to environment.Other values that are substantially equivalent are also within the scopeof the invention. Substantially equivalent refers to quantitative traitsthat when compared do not show statistical differences of their means.XIII. Inbred Corn Plant NL054B

In accordance with one aspect of the present invention, there isprovided a novel inbred corn plant, designated NL054B. Inbred corn plantNL054B is a yellow, dent corn inbred that can be compared to inbred cornplants B73HT, FBLL, and LH132. NL054B differs significantly (at the 5%level) from these inbred lines in several aspects (Table 21A, Table 21B,Table 21C).

TABLE 21A COMPARISON OF NL054B WITH B73HT BARREN DROP EHT MST PHT RTLSHED SILK STL YLD INBRED % % INCH FINAL % INCH % GDU GDU % BU/A NL054B1.1 0.2 32.6 59.0 19.4 69.6 0.4 1574.2 1556.3 1.3 83.0 B73HT 0.8 0.336.7 59.6 21.9 79.9 0.5 1592.3 1599.6 2.0 72.5 DIFF 0.3 −0.1 −4.1 −0.6−2.5 −10.4 −0.1 −18.1 −43.3 −0.8 10.5 # 11 16 16 16 14 16 16 16 16 16 16LOC/TESTS P VALUE 0.85 0.79 0.00** 0.70 0.02* 0.00** 0.90 0.04* 0.00*0.32 0.01** Legend Abbreviations BARREN % = Barren Plants (Percent) DROP% = Dropped Ears (Percent) EHT INCH = Ear Height (Inches) FINAL = FinalStand MST % = Moisture (Percent) PHT INCH = Plant Height (Inches) RTL %= Root Lodging (Percent) SHED GDU = GDUs to Shed SILK GDU = GDUs to SilkSTL % = Stalk Lodging (Percent) YLD BU/A = Yield (Bushels/Acre)Significance levels are indicated as: *5 percent **1 percent

TABLE 21B COMPARISON OF NL054B WITH FBLL BARREN DROP EHT MST PHT RTLSHED SILK STL YLD INBRED % % INCH FINAL % INCH % GDU GDU % BU/A NL054B0.7 0.2 31.8 59.0 20.5 67.6 0.3 1594.5 1578.3 1.1 80.4 FBLL 1.4 0.4 28.057.4 21.1 68.0 0.1 1499.2 1523.6 2.7 75.1 DIFF −0.7 −0.2 3.8 1.6 −0.6−0.4 0.2 95.4 54.7 −1.6 5.3 # 11 15 16 16 15 16 15 16 16 15 16 LOC/TESTSP VALUE 0.72 0.55 0.00** 0.20 0.55 0.82 0.78 0.00** 0.00** 0.53 0.25Legend Abbreviations BARREN % = Barren Plants (Percent) DROP % = DroppedEars (Percent) EHT INCH = Ear Height (Inches) FINAL = Final Stand MST %= Moisture (Percent) PHT INCH = Plant Height (Inches) RTL % = RootLodging (Percent) SHED GDU = GDUs to Shed SILK GDU = GDUs to Silk STL %= Stalk Lodging (Percent) YLD BU/A = Yield (Bushels/Acre) Significancelevels are indicated as: **1 percent

TABLE 21C COMPARISON OF NL054B WITH LH132 BARREN DROP EHT MST PHT RTLSHED SILK STL YLD INBRED % % INCH FINAL % INCH % GDU GDU % BU/A NL054B1.0 0.2 32.2 58.7 20.3 67.6 0.3 1598.1 1580.7 1.2 75.5 LH132 1.9 0.126.3 59.3 18.0 66.0 0.0 1518.4 1522.2 2.0 66.3 DIFF −0.9 0.1 5.8 −0.62.3 1.6 0.3 79.7 58.5 −0.8 9.2 # 19 23 24 24 21 24 23 24 24 23 24LOC/TESTS P VALUE 0.53 0.64 0.00** 0.65 0.01** 0.09+ 0.69 0.00** 0.00**0.54 0.00** Legend Abbreviations BARREN % = Barren Plants (Percent) DROP% = Dropped Ears (Percent) EHT INCH = Ear Height (Inches) FINAL = FinalStand MST % = Moisture (Percent) PHT INCH = Plant Height (Inches) RTL %= Root Lodging (Percent) SHED GDU = GDUs to Shed SILK GDU = GDUs to SilkSTL % = Stalk Lodging (Percent) YLD BU/A = Yield (Bushels/Acre)Significance levels are indicated as: +10 percent **1 percent

A. Origin and Breeding History

Inbred plant NL054B was derived from the cross between two inbredsdesignated B73HT and a line selected from Pioneer hybrid P3378 in thesummer of 1985. B73HT is a publicly available inbred developed at IOWAState University.

NL054B shows uniformity and stability within the limits of environmentalinfluence for the traits described hereinafter in Table 22. NL054B hasbeen self-pollinated and ear-rowed a sufficient number of generationswith careful attention paid to uniformity of plant type to ensurehomozygosity and phenotypic stability. No variant traits have beenobserved or are expected in NL054B.

Inbred corn plants can be reproduced by planting such inbred seeds,growing the resulting corn plants under self-pollinating orsib-pollinating conditions with adequate isolation using standardtechniques well known to an artisan skilled in the agricultural arts.Seeds can be harvested from such a plant using standard, well knownprocedures.

The origin and breeding history of inbred plant NL054B can be summarizedas follows:

Summer 1985

-   -   The inbred line B73HT was crossed to a line selected from the        hybrid Pioneer 3378 (nursery rows 3602 and 3719).

Winter 1985/86

-   -   S₀ seed was grown and self-pollinated (nursery row 70).

Summer 1986

-   -   S₁ seed was grown and self-pollinated (nursery row numbers        4581-4620).

Summer 1987

-   -   S₂ ears were grown on an ear-to-row basis and plants were        self-pollinated (nursery row 401-442).

Winter 1987/88

-   -   S₃ ears were grown and plants were self-pollinated (nursery row        20/30).

Summer 1990

-   -   S₄ ears were grown and plants were selfed (nursery row 1469).

Winter 1990/91

-   -   S₅ ears were grown and plants were self-pollinated (nursery rows        794, L20/33). Selected ears were bulked after harvest and given        the designation NL054B.

B. Phenotypic Description

In accordance with another aspect of the present invention, there isprovided a corn plant having the functional and morphologicalcharacteristics of corn plant NL054B. A description of the functionaland morphological characteristics of corn plant NL054B is presented inTable 22.

TABLE 22 MORPHOLOGICAL TRAITS FOR THE NL054B PHENOTYPE YEAR OF DATA:1992, 1993 CHARACTERISTIC VALUE* 1. Stalk Diameter (Width) cm.  2.7Anthocyanin BASAL-WEAK Nodes With Brace Roots  2.5 Brace Root ColorPURPLE Internode Direction STRAIGHT Internode Length cm. 10.8 2. LeafAngle UPRIGHT Number 19.5 Color MEDIUM GREEN Length cm. 76.5 Width cm. 9.5 Sheath Anthocyanin STRONG Sheath Pubescence HEAVY Marginal WavesFEW Longitudinal Creases ABSENT 3. Tassel Length cm. 34.9 Spike Lengthcm. 18.1 Peduncle Length cm. 11.2 Attitude COMPACT Branch Angle UPRIGHTBranch Number  7.0 Anther Color TAN Glume Color GREEN Glume Band ABSENT4. Ear Silk Color GREEN-YELLOW Number Per Stalk  1.1 Position (Attitude)UPRIGHT Length cm. 17.0 Shape SEMI-CONICAL Diameter cm. 41.5 Weight gm.155.6  Shank Length cm.  8.0 Shank Internode Number  6.7 Husk BractSHORT Husk Cover cm.  4.1 Husk Color Fresh GREEN Husk Color Dry BUFF CobDiameter cm. 24.8 Cob Color WHITE Cob Strength STRONG Shelling Percent85.3 5. Kernel Row Number 16.0 Number Per Row 32.5 Row Direction CURVEDType INTERMEDIATE Cap Color YELLOW Side Color ORANGE Length (Depth) mm.11.4 Width mm.  7.5 Thickness  4.4 Weight of 1000K gm. 312.2  EndospermType NORMAL Endosperm Color YELLOW *These are typical values. Values mayvary due to environment. Other values that are substantially equivalentare also within the scope of the invention. Substantially equivalentrefers to quantitative traits that when compared do not show statisticaldifferences of their means.XIV. Inbred Corn Plant FBMU

In accordance with one aspect of the present invention, there isprovided a novel inbred corn plant, designated FBMU. Inbred corn plantFBMU is a yellow, dent corn inbred that can be compared to inbred cornplants 2FACC, 2FADB, 4676A, and PB80, all of which are proprietaryinbreds developed by DEKALB Genetics Corporation. FBMU differssignificantly (at the 5% level) from these inbred lines in severalaspects (Table 23A, Table 23B, Table 23C, Table 23D).

TABLE 23A COMPARISON OF FBMU WITH 2FACC BARREN DROP EHT MST PHT RTL SHEDSILK STL YLD INBRED % % INCH FINAL % INCH % GDU GDU % BU/A FBMU 0.7 0.527.1 56.9 19.4 67.1 5.4 1421.0 1416.1 5.0 93.6 2FACC 0.5 0.1 27.8 54.420.5 65.7 1.5 1485.3 1484.3 2.5 78.4 DIFF 0.3 0.3 −0.8 2.5 −1.0 1.4 4.0−64.3 −68.2 2.4 15.2 # 29 37 37 39 38 37 37 39 39 38 37 LOC/TESTS PVALUE 0.98 0.25 0.14 0.19 0.05+ 0.15 0.00** 0.00** 0.00** 0.01** 0.00**Legend Abbreviations BARREN % = Barren Plants (Percent) DROP % = DroppedEars (Percent) EHT INCH = Ear Height (Inches) FINAL = Final Stand MST %= Moisture (Percent) PHT INCH = Plant Height (Inches) RTL % = RootLodging (Percent) SHED GDU = GDUs to Shed SILK GDU = GDUs to Silk STL %= Stalk Lodging (Percent) YLD BU/A = Yield (Bushels/Acre) Significancelevels are indicated as: +10 percent **1 percent

TABLE 23B COMPARISON OF FBMU WITH 2FADB BARREN DROP EHT MST PHT RTL SHEDSILK STL YLD INBRED % % INCH FINAL % INCH % GDU GDU % BU/A FBMU 0.6 0.526.3 57.9 19.6 66.8 5.4 1413.9 1408.0 4.5 93.8 2FADB 0.7 0.3 28.5 58.221.6 69.0 1.4 1499.4 1501.8 2.4 81.2 DIFF −0.2 0.2 −2.2 −0.2 −2.0 −2.33.9 −85.5 −93.9 2.1 12.6 # 25 30 30 31 31 30 30 31 31 31 30 LOC/TESTS PVALUE 0.56 0.59 0.00** 0.84 0.00** 0.01** 0.00** 0.00** 0.00** 0.110.00** Legend Abbreviations BARREN % = Barren Plants (Percent) DROP % =Dropped Ears (Percent) EHT INCH = Ear Height (Inches) FINAL = FinalStand MST % = Moisture (Percent) PHT INCH = Plant Height (Inches) RTL %= Root Lodging (Percent) SHED GDU = GDUs to Shed SILK GDU = GDUs to SilkSTL % = Stalk Lodging (Percent) YLD BU/A = Yield (Bushels/Acre)Significance levels are indicated as: **1 percent

TABLE 23C COMPARISON OF FBMU WITH 4676A BARREN DROP EHT MST PHT RTL SHEDSILK STL YLD INBRED % % INCH FINAL % INCH % GDU GDU % BU/A FBMU 0.2 0.026.2 57.3 21.6 66.0 0.8 1351.2 1350.0 1.8 108.0 4676A 2.6 0.1 27.2 58.923.5 64.4 0.1 1357.7 1351.6 1.9 79.6 DIFF −2.4 −0.1 −0.1 −1.6 −1.9 1.60.7 −6.5 −1.6 −0.1 28.4 # 5 10 10 10 10 10 10 10 10 10 10 LOC/TESTS PVALUE 0.00** 0.58 0.35 0.17 0.15 0.30 0.02* 0.57 0.89 0.95 0.00** LegendAbbreviations BARREN % = Barren Plants (Percent) DROP % = Dropped Ears(Percent) EHT INCH = Ear Height (Inches) FINAL = Final Stand MST % =Moisture (Percent) PHT INCH = Plant Height (Inches) RTL % = Root Lodging(Percent) SHED GDU = GDUs to Shed SILK GDU = GDUs to Silk STL % = StalkLodging (Percent) YLD BU/A = Yield (Bushels/Acre) Significance levelsare indicated as: *5 percent **1 percent

TABLE 23D COMPARISON OF FBMU WITH PB80 BARREN DROP EHT MST PUT RTL SHEDSILK STL YLD INBRED % % INCH FINAL % INCH % GDU GDU % BU/A FBMU 0.9 0.626.0 58.5 17.8 67.7 5.7 1439.3 1429.4 5.6 91.2 PB80 2.1 0.6 34.3 58.722.5 72.8 0.3 1570.6 1580.5 4.4 67.1 DIFF −1.3 0.0 −8.3 −0.2 −4.7 −5.05.4 −131.3 −151.0 1.2 24.1 # 14 15 15 16 14 15 15 16 16 16 16 LOC/TESTSP VALUE 0.61 0.97 0.00** 0.79 0.00** 0.00** 0.03* 0.00** 0.00** 0.10+0.00** Legend Abbreviations BARREN % = Barren Plants (Percent) DROP % =Dropped Ears (Percent) EHT INCH = Ear Height (Inches) FINAL = FinalStand MST % = Moisture (Percent) PET INCH = Plant Height (Inches) RTL %= Root Lodging (Percent) SHED GDU = GDUs to Shed SILK GDU = GDUs to SilkSTL % = Stalk Lodging (Percent) YLD BU/A = Yield (Bushels/Acre)Significance levels are indicated as: += 10 percent *5 percent **1percent

A. Origin and Breeding History

Inbred plant FBMU was derived from the cross between two inbredsdesignated PB80 and 4676A. PB80 and 4676A are both proprietary inbredsof DEKALB Genetics Corporation.

FBMU shows uniformity and stability within the limits of environmentalinfluence for the traits described hereinafter in Table 24. FBMU hasbeen self-pollinated and ear-rowed a sufficient number of generationswith careful attention paid to uniformity of plant type to ensurehomozygosity and phenotypic stability. No variant traits have beenobserved or are expected in FBMU.

Inbred corn plants can be reproduced by planting such inbred seeds,growing the resulting corn plants under self-pollinating orsib-pollinating conditions with adequate isolation using standardtechniques well known to an artisan skilled in the agricultural arts.Seeds can be harvested from such a plant using standard, well knownprocedures.

The origin and breeding history of inbred plant FBMU can be summarizedas follows:

Summer 1982

-   -   The cross PB80.4676A was made. PB80 and 4676A are both        proprietary inbreds of DEKALB Genetics Corporation.

Winter 1983

-   -   S₀ seed was grown and self-pollinated (nursery row 770).

Summer 1983

-   -   S₁ ears were grown ear-to-row (nursery rows 701-740).

Summer 1984

-   -   S₂ ears were grown ear-to-row.

Summer 1985

-   -   S₃ ears were grown ear-to-row.

Summer 1986

-   -   S₄ ears were grown ear-to-row.

Summer 1987

-   -   S₅ ears were grown ear-to-row. Bulked S₆ seed was assigned the        inbred code FBMU.

Summer 1988

-   -   FBMU was increased (nursery rows 338-342).

B. Phenotypic Description

In accordance with another aspect of the present invention, there isprovided a corn plant having the functional and morphologicalcharacteristics of corn plant FBMU. A description of the functional andmorphological characteristics of corn plant FBMU is presented in Table24.

TABLE 24 MORPHOLOGICAL TRAITS FOR THE FBMU PHENOTYPE YEAR OF DATA: 1990,1991, 1992, 1993 CHARACTERISTIC VALUE* 1. Stalk Diameter (Width) cm. 2.4 Nodes With Brace Roots  1.5 Brace Root Color GREEN InternodeDirection STRAIGHT Internode Length cm. 12.5 2. Leaf Angle UPRIGHTNumber 19.4 Color MEDIUM GREEN Length cm. 71.4 Width cm.  9.3 SheathPubescence LIGHT Marginal Waves FEW Longitudinal Creases FEW 3. TasselAttitude COMPACT Branch Angle UPRIGHT Length cm. 30.0 Spike Length cm.19.0 Peduncle Length cm.  9.3 Branch Number  5.2 Anther Color RED GlumeColor GREEN Glume Band ABSENT 4. Ear Position (Attitude) UPRIGHT NumberPer Stalk  1.1 Length cm. 17.1 Shape SEMI-CONICAL Diameter cm. 39.8Weight gm. 135.1  Shank Length cm. 12.2 Shank Internode Number  5.8 HuskBract SHORT Husk Cover cm.  3.2 Husk Opening INTERMEDIATE Husk ColorFresh GREEN Husk Color Dry BUFF Cob Diameter cm. 23.2 Cob Color REDShelling percent 83.6 5. Kernel Row Number 13.5 Number Per Row 32.0 RowDirection CURVED Type DENT Cap Color YELLOW Length (Depth) mm. 11.0Width mm.  8.8 Thickness  4.6 Weight of 1000K gm. 311.4  Endosperm TypeNORMAL Endosperm Color YELLOW *These are typical values. Values may varydue to environment. Other values that are substantially equivalent arealso within the scope of the invention. Substantially equivalent refersto quantitative traits that when compared do not show statisticaldifferences of their means.XV. Additional Inbred Corn Plants

The inbred corn plants, 78551S, MM402A, MBZA, 2FADB and 91BMA2 have beenemployed with the corn plants of the present invention in order toproduce several exemplary hybrids. A description of the functional andmorphological characteristics of these corn plants is presented herein.Table 25 concerns 78551S; Table 26 concerns MM402A; Table 27 concernsMBZA; Table 28 concerns 2FADB; and Table 29 concerns 91BMA2. Additionalinformation for these inbred corn plants is presented in the followingco-pending U.S. patent applications: 78551S, Ser. No. 08/181,710, filedJan. 14, 1994; MM402A, Ser. No. 08/181,019, filed Jan. 13, 1994; MBZA,Ser. No. 08/182,616, filed Jan. 14, 1994; 2FADB, Ser. No. 08/130,320,filed Oct. 1, 1993; and 91BMA2, Ser. No. 08/182,476, filed Jan. 13,1994; the disclosures of each of which applications are specificallyincorporated herein by reference.

TABLE 25 MORPHOLOGICAL TRAITS FOR THE 78551S PHENOTYPE YEAR OF DATA:1988, 1989, 1990, 1991, and 1992 CHARACTERISTIC VALUE* 1. Stalk Diameter(Width) cm.  2.4 Nodes With Brace Roots  1.2 Internode DirectionSTRAIGHT Internode Length cm. 14.2 2. Leaf Angle INTERMEDIATE Number17.6 Color MEDIUM GREEN Length cm. 72.6 Width cm.  9.5 3. Tassel Lengthcm. 38.3 Spike Length cm. 30.2 Peduncle Length cm.  6.7 Branch Number 5.4 Anther Color GREEN-YELLOW Glume Band ABSENT 4. Ear Silk Color PINKNumber Per Stalk  1.2 Position (Attitude) UPRIGHT Length cm. 17.8Diameter cm.  3.8 Weight gm. 98.6 Shank Length cm. 13.3 Shank Internodes 6.5 Husk Bract SHORT Husk Cover cm.  6.6 Husk Color Fresh GREEN HuskColor Dry BUFF Cob Diameter cm.  2.2 Cob Color RED Cob Strength WEAKShelling Percent 81.0 5. Kernel Row Number 12.0 Number Per Row 27.7 CapColor YELLOW Side Color ORANGE Length (Depth) mm. 10.2 Width mm.  8.8Thickness  4.9 Weight of 1000K gm. 313.4  Endosperm Type NORMALEndosperm Color YELLOW *These are typical values. Values may vary due toenvironment. Other values that are substantially equivalent are alsowithin the scope of the invention. Substantially equivalent refers toquantitative traits that when compared do not show statisticaldifferences of their means.

TABLE 26 MORPHOLOGICAL TRAITS FOR THE MM402A PHENOTYPE YEAR OF DATA1989, 1990, 1991, and 1992 CHARACTERISTIC VALUE* 1. Stalk Diameter(Width) cm.  2.4 Anthocyanin ABSENT Nodes With Brace Roots  1.5Internode Direction STRAIGHT Internode Length cm. 13.4 2. Leaf AngleUPRIGHT Number 18.6 Length cm. 77.2 Width cm. 10.0 Sheath AnthocyaninABSENT Marginal Waves FEW Longitudinal Creases ABSENT 3. Tassel TotalLength cm. 25.9 Spike Length cm. 24.6 Peduncle Length cm.  7.0 AttitudeCOMPACT Branch Number  1.8 Anther Color GREEN-YELLOW Glume Color GREENGlume Band ABSENT 4. Ear Silk Color GREEN-YELLOW Number Per Stalk  1.0Position (Attitude) UPRIGHT Length cm. 17.6 Shape SEMI-CONICAL Diametercm.  4.3 Weight gm. 131.3  Shank Length cm. 11.2 Shank Internode Number 7.9 Husk Bract SHORT Husk Cover cm.  5.5 Husk Color Fresh GREEN HuskColor Dry BUFF Cob Diameter cm.  2.5 Cob Color WHITE Cob Strength WEAKShelling Percent 88.6 5. Kernel Row Number 18.1 Number Per Row 30.2 RowDirection CURVED Type DENT Cap Color YELLOW Side Color ORANGE Length(Depth) mm. 11.1 Width mm.  7.9 Thickness  4.7 Weight of 1000K gm.276.6  Endosperm Type NORMAL Endosperm Color YELLOW *These are typicalvalues. Values may vary due to environment. Other values that aresubstantially equivalent are also within the scope of the invention.Substantially equivalent refers to quantitative traits that whencompared do not show statistical differences of their means.

TABLE 27 MORPHOLOGICAL TRAITS FOR THE MBZA PHENOTYPE YEAR OF DATA: 1991AND 1992 CHARACTERISTIC VALUE* 1. Stalk Diameter (Width) cm.  2.3Anthocyanin ABSENT Nodes With Brace Roots  1.2 Brace Root Color GREENInternode Length cm. 12.5 2. Leaf Angle UPRIGHT Number 19.0 Color DARKGREEN Length cm. 82.2 Width cm.  9.8 Sheath Anthocyanin BASAL STRONGSheath Pubescence LIGHT Marginal Waves FEW Longitudinal Creases FEW 3.Tassel Length cm. 35.4 Spike Length cm. 26.0 Peduncle Length cm.  5.4Attitude COMPACT Branch Angle UPRIGHT Branch Number  9.3 Anther ColorRED Glume Band ABSENT 4. Ear Silk Color RED Number Per Stalk  1.5 Lengthcm. 16.6 Shape SEMI-CONICAL Diameter cm.  4.0 Weight gm. 114.2  ShankLength cm. 14.5 Shank Internode Number  7.6 Husk Cover  7.1 Husk openingINTERMEDIATE Husk Color Fresh GREEN Husk Color Dry BUFF Cob Diameter cm. 2.2 Cob Color RED Cob Strength WEAK Shelling Percent 85.9 5. Kernel RowNumber 14.9 Number Per Row 35.5 Row Direction CURVED Cap Color YELLOWSide Color DEEP YELLOW Length (Depth) mm. 10.7 Width mm.  7.9 Thickness 3.8 Weight of 1000K gm. 221.7  Endosperm Type NORMAL Endosperm ColorYELLOW *These are typical values. Values may vary due to environment.Other values that are substantially equivalent are also within the scopeof the invention. Substantially equivalent refers to quantitative traitsthat when compared do not show statistical differences of their means.

TABLE 28 MORPHOLOGICAL TRAITS FOR THE 2FADB PHENOTYPE YEAR OF DATA 1991CHARACTERISTIC VALUE 1. Stalk Diameter (Width) cm.  2.0 AnthocyaninBASAL-WEAK Modes With Brace Roots  2.0 Brace Root Color PURPLE InternodeDirection STRAIGHT Internode Length cm. 12.7 2. Leaf Angle UPRIGHTNumber 19.0 Color MEDIUM GREEN Length cm. 72.8 Width cm.  8.6 SheathAnthocyanin STRONG Sheath Pubescence LIGHT Marginal Waves FEWLongitudinal Creases ABSENT 3. Tassel Length cm. 27.4 Spike Length cm.20.1 Peduncle Length cm.  6.5 Attitude COMPACT Branch Angle UPRIGHTBranch Number  7.8 Anther Color RED Glume Color GREEN Glume Band ABSENT4. Ear Silk Color TAN Number Per Stalk  1.2 Position (Attitude) UPRIGHTLength cm. 13.6 Shape SEMI-CONICAL Diameter cm.  4.1 Weight gm. 108.0 Shank Length cm. 13.8 Shank Internode Number  8.2 Husk Bract SHORT HuskCover cm.  5.7 Husk Opening INTERMEDIATE Husk Color Fresh LIGHT GREENHusk Color Dry BUFF Cob Diameter cm.  2.3 Cob Color RED Cob StrengthSTRONG Shelling Percent 84.1 5. Kernel Row Number 14.4 Number Per Row27.4 Row Direction CURVED Type DENT Cap Color YELLOW Side ColorDEEP-YELLOW Length (Depth) mm. 11.2 Width mm.  8.2 Thickness  4.1 Weightof 1000K gm. 290.3  Endosperm Type NORMAL Endosperm Color YELLOW

TABLE 29 MORPHOLOGICAL TRAITS FOR THE 91BMA2 PHENOTYPE YEAR OF DATA:1991 AND 1992 CHARACTERISTIC VALUE* 1. Stalk Diameter (Width) cm.  1.8Anthocyanin ABSENT Nodes With Brace Roots  1.1 Internode DirectionSTRAIGHT Internode Length cm. 12.1 2. Leaf Angle INTERMEDIATE Number19.0 Color MEDIUM GREEN Length cm. 65.5 Width cm.  8.4 Sheath PubescenceLIGHT Marginal Waves FEW Longitudinal Creases FEW 3. Tassel Length cm.32.0 Spike Length cm. 23.2 Peduncle Length cm.  7.7 Branch Angle UPRIGHTBranch Number  4.0 Glume Color GREEN Glume Band ABSENT 4. Ear Silk ColorGREEN YELLOW Number Per Stalk  1.1 Position (Attitude) UPRIGHT Lengthcm. 13.5 Shape SEMI-CONICAL Diameter cm.  3.4 Weight gm. 92.5 ShankLength cm. 13.9 Shank Internode Number  6.4 Husk Bract SHORT Husk Cover 5.9 Husk Color Fresh GREEN Husk Color Dry BUFF Cob Diameter cm.  2.0Cob Color RED Shelling Percent 82.7 5. Kernel Row Number 12.7 Number PerRow 27.8 Row Direction CURVED Type INTERMEDIATE Cap Color YELLOW SideColor ORANGE Length (Depth) mm.  9.6 Width mm.  7.4 Thickness  3.5Weight of 1000K gm. 225.3  Endosperm Type NORMAL Endosperm Color YELLOW*These are typical values. Values may vary due to environment. Othervalues that are substantially equivalent are also within the scope ofthe invention. Substantially equivalent refers to quantitative traitsthat when compared do not show statistical differences of their means.

For convenience, the following table, Table 30, sets forth the exemplaryhybrids produced using the inbreds of the present invention. Theparticular tables of the present specification that set forth themorphological characteristics, RFLP and isoenzyme marker profiles of theinbred parents and exemplary hybrid corn plants are referenced withinTable 30.

TABLE 30 INBRED AND HYBRID TABLE CROSS-REFERENCES MORPH- ISO- MORPH-ISO- EXEM- COMPAR- MORPH- ISO- 1st OLOGICAL RFLP ZYME 2nd OLOGICAL RFLPZYME PLARY ISON OLOGICAL RFLP ZYME INBRED TRAITS DATA DATA INBRED TRAITSDATA DATA HYBRID DATA TRAITS DATA DATA GMLEA 2 52 64 6F905 4 53 65 DK70632 42 76 86 6F905 4 53 65 GMLEA 2 52 64 DK706 32 42 76 86 91CSV-1 6 5466 01ASB1 18 60 72 DK446 33 43 77 87 91DFA-5 8 55 67 78551S 25 DX474 3747 81 91 WDAQ2 10 56 68 01IBH2 20 61 73 DK604 34 44 78 88 WDDQ1 12 57 69MM402A 26 DK642 38 48 82 92 85DGD1 14 58 70 MBZA 27 DK560 39 49 83 93PHEI4 16 59 71 2FADB 28 DX566 40 50 84 94 01ASB1 18 60 72 91CSV-1 6 5466 DK446 33 43 77 87 01IBH2 20 61 73 9BMA2 29 DK442 36 46 80 90 WDAQ2 1056 68 DK604 34 44 78 88 FBMU 24 63 75 DK527 35 45 79 89 NL054B 22 62 74MM402A 26 DK626 41 52 85 95 FBHU 24 63 75 01IBH2 20 61 73 DK527 35 45 7989XVI. Single Gene Conversions

When the term inbred corn plant is used in the context of the presentinvention, this also includes any single gene conversions of thatinbred. The term single gene converted plant as used herein refers tothose corn plants which are developed by a plant breeding techniquecalled backcrossing wherein essentially all of the desired morphologicaland physiological characteristics of an inbred are recovered in additionto the single gene transferred into the inbred via the backcrossingtechnique. Backcrossing methods can be used with the present inventionto improve or introduce a characteristic into the inbred. The termbackcrossing as used herein refers to the repeated crossing of a hybridprogeny back to one of the parental corn plants for that inbred. Theparental corn plant which contributes the gene for the desiredcharacteristic is termed the nonrecurrent or donor parent. Thisterminology refers to the fact that the nonrecurrent parent is used onetime in the backcross protocol and therefore does not recur. Theparental corn plant to which the gene or genes from the nonrecurrentparent are transferred is known as the recurrent parent as it is usedfor several rounds in the backcrossing protocol (Poehlman & Sleper,1994; Fehr, 1987). In a typical backcross protocol, the original inbredof interest (recurrent parent) is crossed to a second inbred(nonrecurrent parent) that carries the single gene of interest to betransferred. The resulting progeny from this cross are then crossedagain to the recurrent parent and the process is repeated until a cornplant is obtained wherein essentially all of the desired morphologicaland physiological characteristics of the recurrent parent are recoveredin the converted plant, in addition to the single transferred gene fromthe nonrecurrent parent.

The selection of a suitable recurrent parent is an important step for asuccessful backcrossing procedure. The goal of a backcross protocol isto alter or substitute a single trait or characteristic in the originalinbred. To accomplish this, a single gene of the recurrent inbred ismodified or substituted with the desired gene from the nonrecurrentparent, while retaining essentially all of the rest of the desiredgenetic, and therefore the desired physiological and morphological,constitution of the original inbred. The choice of the particularnonrecurrent parent will depend on the purpose of the backcross, one ofthe major purposes is to add some commercially desirable, agronomicallyimportant trait to the plant. The exact backcrossing protocol willdepend on the characteristic or trait being altered to determine anappropriate testing protocol. Although backcrossing methods aresimplified when the characteristic being transferred is a dominantallele, a recessive allele may also be transferred. In this instance itmay be necessary to introduce a test of the progeny to determine if thedesired characteristic has been successfully transferred.

Many single gene traits have been identified that are not regularlyselected for in the development of a new inbred but that can be improvedby backcrossing techniques. Examples of these traits include but are notlimited to, male sterility, waxy starch, herbicide resistance,resistance for bacterial, fungal, or viral disease, insect resistance,male fertility, enhanced nutritional quality, and industrial usage.These genes are generally inherited through the nucleus. Some knownexceptions to this are the genes for male sterility, some of which areinherited cytoplasmically, but still act as single gene traits. Severalof these single gene traits are described in U.S. Ser. No. 07/113,561,filed Aug. 25, 1993, the disclosure of which is specifically herebyincorporated by reference.

Direct selection may be applied where the single gene acts as a dominanttrait. An example might be the herbicide resistance trait. For thisselection process, the progeny of the initial cross are sprayed with theherbicide prior to the backcrossing. The spraying eliminates any plantswhich do not have the desired herbicide resistance characteristic, andonly those plants which have the herbicide resistance gene are used inthe subsequent backcross. This process is then repeated for alladditional backcross generations.

The waxy characteristic is an example of a recessive trait. In thisexample, the progeny resulting from the first backcross generation (BC1)must be grown and selfed. A test is then run on the selfed seed from theBC1 plant to determine which BC1 plants carried the recessive gene forthe waxy trait. In other recessive traits, additional progeny testing,for example growing additional generations such as the BC1S1 may berequired to determine which plants carry the recessive gene.

XVII. Origin and Breeding History of an Exemplary Single Gene ConvertedPlant

85DGD1 MLms is a single gene conversion of 85DGD1 to cytoplasmic malesterility. 85DGD1 MLms was derived using backcross methods. 85DGD1 (aproprietary inbred of DEKALB Genetics Corporation) was used as therecurrent parent and MLms, a germplasm source carrying ML cytoplasmicsterility, was used as the nonrecurrent parent. The breeding history ofthe single gene converted inbred 85DGD1 MLms can be summarized asfollows:

Hawaii Nurseries Planting Date Apr. 2, 1992

-   -   Made up S—O: Female row 585 male row 500

Hawaii Nurseries Planting Date Jul. 15, 1992

-   -   S—O was grown and plants were backcrossed times 85DGD1 (rows        444×443)

Hawaii Nurseries Planting Date Nov. 18, 1992

-   -   Bulked seed of the BC1 was grown and backcrossed times 85DGD1        (rows V3-27×V3-26)

Hawaii Nurseries Planting Date Apr. 2, 1993

-   -   Bulked seed of the BC2 was grown and backcrossed times 85DGD1        (rows 37×36)

Hawaii Nurseries Planting Date Jul. 14, 1993

-   -   Bulked seed of the BC3 was grown and backcrossed times 85DGD1        (rows 99×98)

Hawaii Nurseries Planting Date Oct. 28, 1993

-   -   Bulked seed of BC4 was grown and backcrossed times 85DGD1 (rows        KS-63×KS-62)

Summer 1994

-   -   A single ear of the BC5 was grown and backcrossed times 85DGD1        (MC94-822×MC94-822-7)

Winter 1994

-   -   Bulked seed of the BC6 was grown and backcrossed times 85DGD1        (3Q-1×3Q-2)

Summer 1995

-   -   Seed of the BC7 was bulked and named 85DGD1 MLms.        XVIII. Tissue Culture and in vitro Regeneration of Corn Plants

A further aspect of the invention relates to tissue culture of cornplants designated, separately, GMLEA, 6F905, 91CSV-1, 91DFA-5, WDAQ2,WDDQ1, 85DGD1, PHEI4, 01ASB1, 01IBH2, NL054B or FBMU. As used herein,the term “tissue culture” indicates a composition comprising isolatedcells of the same or a different type or a collection of such cellsorganized into parts of a plant. Exemplary types of tissue cultures areplant protoplast, plant calli, plant clumps, and plant cells that areintact in plants or parts of plants, such as embryos, pollen, flowers,kernels, ears, cobs, leaves, husks, stalks, roots, root tips, anthers,silk and the like. In a preferred embodiment, tissue culture is embryos,protoplast, meristematic cells, pollen, leaves or anthers. Means forpreparing and maintaining plant tissue culture are well known in theart. By way of example, a tissue culture comprising organs such astassels or anthers, has been used to produce regenerated plants. (See,U.S. patent applications Ser. No. 07/992,637, filed Dec. 18, 1992 andSer. No. 07/995,938, filed Dec. 21, 1992, now issued as U.S. Pat. No.5,322,789, issued Jun. 21, 1994, the disclosures of which areincorporated herein by reference).

A. Tassel/Anther Culture

Tassels contain anthers which in turn enclose microspores. Microsporesdevelop into pollen. For anther/microspore culture, if tassels are theplant composition, they are preferably selected at a stage when themicrospores are uninucleate, that is, include only one, rather than 2 or3 nuclei. Methods to determine the correct stage are well known to thoseskilled in the art and include mitramycin fluorescent staining (Pace etal., 1987), trypan blue (preferred) and acetocarmine squashing. Themid-uninucleate microspore stage has been found to be the developmentalstage most responsive to the subsequent methods disclosed to ultimatelyproduce plants.

Although microspore-containing plant organs such as tassels cangenerally be pretreated at any cold temperature below about 25° C., arange of 4 to 25° C. is preferred, and a range of 8 to 14° C. isparticularly preferred. Although other temperatures yield embryoids andregenerated plants, cold temperatures produce optimum response ratescompared to pretreatment at temperatures outside the preferred range.Response rate is measured as either the number of embryoids or thenumber of regenerated plants per number of microspores initiated inculture.

Although not required, when tassels are employed as the plant organ, itis generally preferred to sterilize their surface. Following surfacesterilization of the tassels, for example, with a solution of calciumhypochloride, the anthers are removed from about 70 to 150 spikelets(small portions of the tassels) and placed in a preculture orpretreatment medium. Larger or smaller amounts can be used depending onthe number of anthers.

When one elects to employ tassels directly, tassels are preferablypretreated at a cold temperature for a predefined time, preferably at10° C. for about 4 days. After pretreatment of a whole tassel at a coldtemperature, dissected anthers are further pretreated in an environmentthat diverts microspores from their developmental pathway. The functionof the preculture medium is to switch the developmental program from oneof pollen development to that of embryoid/callus development. Anembodiment of such an environment in the form of a preculture mediumincludes a sugar alcohol, for example mannitol or sorbitol, inositol orthe like. An exemplary synergistic combination is the use of mannitol ata temperature of about 10° C. for a period ranging from about 10 to 14days. In a preferred embodiment, 3 ml of 0.3 M mannitol combined with 50mg/l of ascorbic acid, silver nitrate and colchicine is used forincubation of anthers at 10° C. for between 10 and 14 days. Anotherembodiment is to substitute sorbitol for mannitol. The colchicineproduces chromosome doubling at this early stage. The chromosomedoubling agent is preferably only present at the preculture stage.

It is believed that the mannitol or other similar carbon structure orenvironmental stress induces starvation and functions to forcemicrospores to focus their energies on entering developmental stages.The cells are unable to use, for example, mannitol as a carbon source atthis stage. It is believed that these treatments confuse the cellscausing them to develop as embryoids and plants from microspores.Dramatic increases in development from these haploid cells, as high as25 embryoids in 10⁴ microspores, have resulted from using these methods.

In embodiments where microspores are obtained from anthers, microsporescan be released from the anthers into an isolation medium following themannitol preculture step. One method of release is by disruption of theanthers, for example, by chopping the anthers into pieces with a sharpinstrument, such as a razor blade, scalpel or Waring blender. Theresulting mixture of released microspores, anther fragments andisolation medium are then passed through a filter to separatemicrospores from anther wall fragments. An embodiment of a filter is amesh, more specifically, a nylon mesh of about 112 μm pore size. Thefiltrate which results from filtering the microspore-containing solutionis preferably relatively free of anther fragments, cell walls and otherdebris.

In a preferred embodiment, isolation of microspores is accomplished at atemperature below about 25° C. and, preferably at a temperature of lessthan about 15° C. Preferably, the isolation media, dispersing tool(e.g., razor blade) funnels, centrifuge tubes and dispersing container(e.g., petri dish) are all maintained at the reduced temperature duringisolation. The use of a precooled dispersing tool to isolate maizemicrospores has been reported (Gaillard et al., 1991).

Where appropriate and desired, the anther filtrate is then washedseveral times in isolation medium. The purpose of the washing andcentrifugation is to eliminate any toxic compounds which are containedin the non-microspore part of the filtrate and are created by thechopping process. The centrifugation is usually done at decreasing spinspeeds, for example, 1000, 750, and finally 500 rpms.

The result of the foregoing steps is the preparation of a relativelypure tissue culture suspension of microspores that are relatively freeof debris and anther remnants.

To isolate microspores, an isolation media is preferred. An isolationmedia is used to separate microspores from the anther walls whilemaintaining their viability and embryogenic potential. An illustrativeembodiment of an isolation media includes a 6 percent sucrose or maltosesolution combined with an antioxidant such as 50 mg/l of ascorbic acid,0.1 mg/l biotin and 400 mg/l of proline, combined with 10 mg/l ofnicotinic acid and 0.5 mg/l AgNO₃. In another embodiment, the biotin andproline are omitted.

An isolation media preferably has a higher antioxidant level where usedto isolate microspores from a donor plant (a plant from which a plantcomposition containing a microspore is obtained) that is field grown incontrast to greenhouse grown. A preferred level of ascorbic acid in anisolation medium is from about 50 mg/l to about 125 mg/l and, morepreferably from about 50 mg/l to about 100 mg/l.

One can find particular benefit in employing a support for themicrospores during culturing and subculturing. Any support thatmaintains the cells near the surface can be used. The microsporesuspension is layered onto a support, for example by pipetting. Thereare several types of supports which are suitable and are within thescope of the invention. An illustrative embodiment of a solid support isa TRANSWELL® culture dish. Another embodiment of a solid support fordevelopment of the microspores is a bilayer plate wherein liquid mediais on top of a solid base. Other embodiments include a mesh or amillipore filter. Preferably, a solid support is a nylon mesh in theshape of a raft. A raft is defined as an approximately circular supportmaterial which is capable of floating slightly above the bottom of atissue culture vessel, for example, a petri dish, of about a 60 or 100mm size, although any other laboratory tissue culture vessel willsuffice. In an illustrative embodiment, a raft is about 55 mm indiameter.

Culturing isolated microspores on a solid support, for example, on a 10μm pore nylon raft floating on 2.2 ml of medium in a 60 mm petri dish,prevents microspores from sinking into the liquid medium and thusavoiding low oxygen tension. These types of cell supports enable theserial transfer of the nylon raft with its associatedmicrospore/embryoids ultimately to full strength medium containingactivated charcoal and solidified with, for example, GELRITE™(solidifying agent). The charcoal is believed to absorb toxic wastes andintermediaries. The solid medium allows embryoids to mature.

The liquid medium passes through the mesh while the microspores areretained and supported at the medium-air interface. The surface tensionof the liquid medium in the petri dish causes the raft to float. Theliquid is able to pass through the mesh: consequently, the microsporesstay on top. The mesh remains on top of the total volume of liquidmedium. An advantage of the raft is to permit diffusion of nutrients tothe microspores. Use of a raft also permits transfer of the microsporesfrom dish to dish during subsequent subculture with minimal loss,disruption or disturbance of the induced embryoids that are developing.The rafts represent an advantage over the multi-welled TRANSWELL®plates, which are commercially available from COSTAR, in that thecommercial plates are expensive. Another disadvantage of these plates isthat to achieve the serial transfer of microspores to subsequent media,the membrane support with cells must be peeled off the insert in thewells. This procedure does not produce as good a yield nor as efficienttransfers, as when a mesh is used as a vehicle for cell transfer.

The culture vessels can be further defined as either (1) a bilayer 60 mmpetri plate wherein the bottom 2 ml of medium are solidified with 0.7percent agarose overlaid with 1 mm of liquid containing the microspores;(2) a nylon mesh raft wherein a wafer of nylon is floated on 1.2 ml ofmedium and 1 ml of isolated microspores is pipetted on top; or (3)TRANSWELL® plates wherein isolated microspores are pipetted ontomembrane inserts which support the microspores at the surface of 2 ml ofmedium.

After the microspores have been isolated, they are cultured in a lowstrength anther culture medium until about the 50 cell stage when theyare subcultured onto an embryoid/callus maturation medium. Medium isdefined at this stage as any combination of nutrients that permit themicrospores to develop into embryoids or callus. Many examples ofsuitable embryoid/callus promoting media are well known to those skilledin the art. These media will typically comprise mineral salts, a carbonsource, vitamins, growth regulations. A solidifying agent is optional. Apreferred embodiment of such a media is referred to by the inventor asthe “D medium” which typically includes 6N1 salts, AgNO₃ and sucrose ormaltose.

In an illustrative embodiment, 1 ml of isolated microspores are pipettedonto a 10 μm nylon raft and the raft is floated on 1.2 ml of medium “D”,containing sucrose or, preferably maltose. Both calli and embryoids candevelop. Calli are undifferentiated aggregates of cells. Type I is arelatively compact, organized and slow growing callus. Type II is asoft, friable and fast-growing one. Embryoids are aggregates exhibitingsome embryo-like structures. The embryoids are preferred for subsequentsteps to regenerating plants. Culture medium “D” is an embodiment ofmedium that follows the isolation medium and replaces it. Medium “D”promotes growth to an embryoid/callus. This medium comprises 6N1 saltsat ⅛ the strength of a basic stock solution, (major components) andminor components, plus 12 percent sucrose or, preferably 12 percentmaltose, 0.1 mg/l B1, 0.5 mg/l nicotinic acid, 400 mg/l proline and 0.5mg/l silver nitrate. Silver nitrate is believed to act as an inhibitorto the action of ethylene. Multi-cellular structures of approximately 50cells each generally arise during a period of 12 days to 3 weeks. Serialtransfer after a two week incubation period is preferred.

After the petri dish has been incubated for an appropriate period oftime, preferably two weeks, in the dark at a predefined temperature, araft bearing the dividing microspores is transferred serially to solidbased media which promotes embryo maturation. In an illustrativeembodiment, the incubation temperature is 30° C. and the mesh raftsupporting the embryoids is transferred to a 100 mm petri dishcontaining the 6N1-TGR-4P medium, an “anther culture medium.” Thismedium contains 6N1 salts, supplemented with 0.1 mg/l TIBA, 12 percentsugar (sucrose, maltose or a combination thereof), 0.5 percent activatedcharcoal, 400 mg/l proline, 0.5 mg/l B, 0.5 mg/l nicotinic acid, and 0.2percent GELRITE™ (solidifying agent) and is capable of promoting thematuration of the embryoids. Higher quality embryoids, that is,embryoids which exhibit more organized development, such as better shootmeristem formation without precocious germination were typicallyobtained with the transfer to full strength medium compared to thoseresulting from continuous culture using only, for example, the isolatedmicrospore culture (IMC) Medium “D.” The maturation process permits thepollen embryoids to develop further in route toward the eventualregeneration of plants. Serial transfer occurs to full strengthsolidified 6N1 medium using either the nylon raft, the TRANSWELL®membrane or bilayer plates, each one requiring the movement ofdeveloping embryoids to permit further development into physiologicallymore mature structures.

In an especially preferred embodiment, microspores are isolated in anisolation media comprising about 6 percent maltose, cultured for abouttwo weeks in an embryoid/calli induction medium comprising about 12percent maltose and then transferred to a solid medium comprising about12 percent sucrose.

At the point of transfer of the raft after about two weeks incubation,embryoids exist on a nylon support. The purpose of transferring the raftwith the embryoids to a solidified medium after the incubation is tofacilitate embryo maturation. Mature embryoids at this point areselected by visual inspection indicated by zygotic embryo-likedimensions and structures and are transferred to the shoot initiationmedium. It is preferred that shoots develop before roots, or that shootsand roots develop concurrently. If roots develop before shoots, plantregeneration can be impaired. To produce solidified media, the bottom ofa petri dish of approximately 100 mm is covered with about 30 ml of 0.2percent GELRITE™ (solidifying agent) solidified medium. A sequence ofregeneration media are used for whole plant formation from theembryoids.

During the regeneration process, individual embryoids are induced toform plantlets. The number of different media in the sequence can varydepending on the specific protocol used. Finally, a rooting medium isused as a prelude to transplanting to soil. When plantlets reach aheight of about 5 cm, they are then transferred to pots for furthergrowth into flowering plants in a greenhouse by methods well known tothose skilled in the art.

Plants have been produced from isolated microspore cultures by methodsdisclosed herein, including self-pollinated plants. The rate of embryoidinduction was much higher with the synergistic preculture treatmentconsisting of a combination of stress factors, including a carbon sourcewhich can be capable of inducing starvation, a cold temperature andcolchicine, than has previously been reported. An illustrativeembodiment of the synergistic combination of treatments leading to thedramatically improved response rate compared to prior methods, is atemperature of about 10° C., mannitol as a carbon source, and 0.05percent colchicine.

The inclusion of ascorbic acid, an anti-oxidant, in the isolation mediumis preferred for maintaining good microspore viability. However, thereseems to be no advantage to including mineral salts in the isolationmedium. The osmotic potential of the isolation medium was maintainedoptimally with about 6 percent sucrose, although a range of 2 percent to12 percent is within the scope of this invention.

In an embodiment of the embryoid/callus organizing media, mineral saltsconcentration in IMC Culture Media “D” is (⅛×), the concentration whichis used also in anther culture medium. The 6N1 salts major componentshave been modified to remove ammonium nitrogen. Osmotic potential in theculture medium is maintained with about 12 percent sucrose and about 400mg/l proline. Silver nitrate (0.5 mg/l) was included in the medium tomodify ethylene activity. The preculture media is further characterizedby having a pH of about 5.7 to 6.0. Silver nitrate and vitamins do notappear to be crucial to this medium but do improve the efficiency of theresponse.

Whole anther cultures can also be used in the production ofmonocotyledonous plants from a plant culture system. There are somebasic similarities of anther culture methods and microspore culturemethods with regard to the media used. A difference from isolatedmicrospore cultures is that undisrupted anthers are cultured, so that asupport, e.g. a nylon mesh support, is not needed. The first step indeveloping the anther cultures is to incubate tassels at a coldtemperature. A cold temperature is defined as less than about 25° C.More specifically, the incubation of the tassels is preferably performedat about 10° C. A range of 8 to 14° C. is also within the scope of theinvention. The anthers are then dissected from the tassels, preferablyafter surface sterilization using forceps, and placed on solidifiedmedium. An example of such a medium is designated by the inventors as6N1-TGR-P4.

The anthers are then treated with environmental conditions that arecombinations of stresses that are capable of diverting microspores fromgametogenesis to embryogenesis. It is believed that the stress effect ofsugar alcohols in the preculture medium, for example, mannitol, isproduced by inducing starvation at the predefined temperature. In oneembodiment, the incubation pretreatment is for about 14 days at 10° C.It was found that treating the anthers in addition with a carbonstructure, an illustrative embodiment being a sugar alcohol, preferably,mannitol, produces dramatically higher anther culture response rates asmeasured by the number of eventually regenerated plants, than bytreatment with either cold treatment or mannitol alone. These resultsare particularly surprising in light of teachings that cold is betterthan mannitol for these purposes, and that warmer temperatures interactwith mannitol better.

To incubate the anthers, they are floated on a preculture medium whichdiverts the microspores from gametogenesis, preferably on a mannitolcarbon structure, more specifically, 0.3 M of mannitol plus 50 mg/l ofascorbic acid. 3 ml is about the total amount in a dish, for example, atissue culture dish, more specifically, a 60 mm petri dish. Anthers areisolated from about 120 spikelets for one dish yields about 360 anthers.

Chromosome doubling agents can be used in the preculture media foranther cultures. Several techniques for doubling chromosome number(Jensen, 1974; Wan et al., 1989) have been described. Colchicine is oneof the doubling agents. However, developmental abnormalities arisingfrom in vitro cloning are further enhanced by colchicine treatments, andprevious reports indicated that colchicine is toxic to microspores. Theaddition of colchicine in increasing concentrations during mannitolpretreatment prior to anther culture and microspore culture has achievedimproved percentages.

An illustrative embodiment of the combination of a chromosome doublingagent and preculture medium is one which contains colchicine. In aspecific embodiment, the colchicine level is preferably about 0.05percent. The anthers remain in the mannitol preculture medium with theadditives for about 10 days at 10° C. Anthers are then placed onmaturation media, for example, that designated 6N1-TGR-P4, for 3 to 6weeks to induce embryoids. If the plants are to be regenerated from theembryoids, shoot regeneration medium is employed, as in the isolatedmicrospore procedure described in the previous sections. Otherregeneration media can be used sequentially to complete regeneration ofwhole plants.

The anthers are then exposed to embryoid/callus promoting medium, forexample, that designated 6N1-TGR-P4 to obtain callus or embryoids. Theembryoids are recognized by identification visually of embryonic-likestructures. At this stage, the embryoids are transferred serially to aseries of regeneration media. In an illustrative embodiment, the shootinitiation medium comprises BAP (6-benzyl-amino-purine) and NAA(naphthalene acetic acid). Regeneration protocols for isolatedmicrospore cultures and anther cultures are similar.

B. Other Cultures and Regeneration

The present invention contemplates a corn plant regenerated from atissue culture of an inbred (e.g. GMLEA, 6F905, 91CSV-1, 91DFA-5, WDAQ2,WDDQ1, 85DGD1, PHEI4, 01ASB1, 01IBH2, NL054B or FBMU) or hybrid plant(DK706, DK446, DK474, DK642, DK604, DK560, DK566, DK442, DK527, DK626)of the present invention. As is well known in the art, tissue culture ofcorn can be used for the in vitro regeneration of a corn plant. By wayof example, a process of tissue culturing and regeneration of corn isdescribed in European Patent Application, publication 160,390, thedisclosure of which is incorporated by reference. Corn tissue cultureprocedures are also described in Green & Rhodes (1982) and Duncan etal., (1985). The study by Duncan indicates that 97 percent of culturedplants produced calli capable of regenerating plants. Subsequent studieshave shown that both inbreds and hybrids produced 91 percent regenerablecalli that produced plants.

Other studies indicate that non-traditional tissues are capable ofproducing somatic embryogenesis and plant regeneration. See. e.g.Songstad et al. (1986), and Conger et al. (1987), the disclosures ofwhich are incorporated herein by reference.

Briefly, by way of example, to regenerate a plant of this invention,cells are selected following growth in culture. Where employed, culturedcells are preferably grown either on solid supports or in the form ofliquid suspensions as set forth above. In either instance, nutrients areprovided to the cells in the form of media, and environmental conditionsare controlled. There are many types of tissue culture media comprisingamino acids, salts, sugars, hormones and vitamins. Most of the mediaemployed to regenerate inbred and hybrid plants have some similarcomponents, the media differ in the composition and proportions of theiringredients depending on the particular application envisioned. Forexample, various cell types usually grow in more than one type of media,but exhibit different growth rates and different morphologies, dependingon the growth media. In some media, cells survive but do not divide.Various types of media suitable for culture of plant cells have beenpreviously described and discussed above.

An exemplary embodiment for culturing recipient corn cells in suspensioncultures includes using embryogenic cells in Type II (Armstrong & Green,1985; Gordon-Kamm et al., 1990) callus, selecting for small (10 to 30μ)isodiametric, cytoplasmically dense cells, growing the cells insuspension cultures with hormone containing media, subculturing into aprogression of media to facilitate development of shoots and roots, andfinally, hardening the plant and readying it metabolically for growth insoil.

Meristematic cells (i.e., plant cells capable of continual cell divisionand characterized by an undifferentiated cytological appearance,normally found at growing points or tissues in plants such as root tips,stem apices, lateral buds, etc.) can be cultured.

Embryogenic calli are produced (Gordon-Kamm et al., 1990). Specifically,plants from hybrids produced from crossing an inbred of the presentinvention with another inbred are grown to flowering in a greenhouse.Explants from at least one of the following F₁ tissues: the immaturetassel tissue, intercalary meristems and leaf bases, apical meristems,and immature ears are placed in an initiation medium which contain MSsalts, supplemented with thiamine, agar, and sucrose. Cultures areincubated in the dark at about 23° C. All culture manipulations andselections are performed with the aid of a dissecting microscope.

After about 5 to 7 days, cellular outgrowths are observed from thesurface of the explants. After about 7 to 21 days, the outgrowths aresubcultured by placing them into fresh medium of the same composition.Some of the intact immature embryo explants are placed on fresh medium.Several subcultures later (after about 2 to 3 months) enough material ispresent from explants for subdivision of these embryogenic calli intotwo or more pieces.

Callus pieces from different explants are not mixed. After furthergrowth and subculture (about 6 months after embryogenic callusinitiation), there are usually between 1 and 100 pieces derivedultimately from each selected explant. During this time of cultureexpansion, a characteristic embryogenic culture morphology develops as aresult of careful selection at each subculture. Any organized structuresresembling roots or root primordia are discarded. Material known fromexperience to lack the capacity for sustained growth is also discarded(translucent, watery, embryogenic structures). Structures with a firmconsistency resembling at least in part the scutulum of the in vivoembryo are selected.

The callus is maintained on agar-solidified MS-type media. A preferredhormone is 2,4-D. Visual selection of embryo-like structures is done toobtain subcultures. Transfer of material other than that displayingembryogenic morphology results in loss of the ability to recover wholeplants from the callus. Some calli exhibit somaclonal variation. Theseare phenotypic changes appearing in culture.

Cell suspensions are prepared from the calli by selecting cellpopulations that appear homogeneous macroscopically. A portion of thefriable, rapidly growing embryogenic calli is inoculated into MS Medium.The calli in medium are incubated at about 27° C. on a gyrotary shakerin the dark or in the presence of low light. The resultant suspensionculture is transferred about once every seven days by taking about 5 to10 ml of the culture and introducing this inoculum into fresh medium ofthe composition listed above.

For regeneration, embryos which appear on the callus surface areselected and regenerated into whole plants by transferring theembryogenic structures into a sequence of solidified media which includedecreasing concentrations of 2,4-D or other auxins. Other hormones whichcan be used in the media include dicamba, NAA, ABA, BAP, and 2-NCA. Thereduction is relative to the concentration used in culture maintenancemedia. Plantlets are regenerated from these embryos by transfer to ahormone-free medium, subsequently transferred to soil, and grown tomaturity.

Progeny are produced by taking pollen and selfing, backcrossing orsibling regenerated plants by methods well known to those skilled in thearts. Seeds are collected from the regenerated plants.

XIX. Processes of Preparing Corn Plants and the Corn Plants Produced bysuch Process

The present invention also provides a process of preparing a novel cornplant and a corn plant produced by such a process. In accordance withsuch a process, a first parent corn plant is crossed with a secondparent corn plant wherein at least one of the first and second cornplants is the inbred corn plant GMLEA, 6F905, 91CSV-1, 91DFA-5, WDAQ2,WDDQ1, 85DGD1, PHEI4, 01ASB1, 01IBH2, NL054B or FBMU. In one embodiment,a corn plant prepared by such a process is a first generation F₁ hybridcorn plant prepared by a process wherein both the first and secondparent corn plants are inbred corn plants.

Corn plants (Zea mays L.) can be crossed by either natural or mechanicaltechniques. Natural pollination occurs in corn when wind blows pollenfrom the tassels to the silks that protrude from the tops of theincipient ears. Mechanical pollination can be effected either bycontrolling the types of pollen that can blow onto the silks or bypollinating by hand.

In a preferred embodiment, crossing comprises the steps of:

-   -   (a) planting in pollinating proximity seeds of a first and a        second parent corn plant, and preferably, seeds of a first        inbred corn plant and a second, distinct inbred corn plant;    -   (b) cultivating or growing the seeds of the first and second        parent corn plants into plants that bear flowers;    -   (c) emasculating flowers of either the first or second parent        corn plant, i.e., treating the flowers so as to prevent pollen        production, in order to produce an emasculated parent corn        plant;    -   (d) allowing natural cross-pollination to occur between the        first and second parent corn plant;    -   (e) harvesting seeds produced on the emasculated parent corn        plant; and, where desired,    -   (f) growing the harvested seed into a corn plant, or preferably,        a hybrid corn plant.

Parental plants are planted in pollinating proximity to each other byplanting the parental plants in alternating rows, in blocks or in anyother convenient planting pattern. Plants of both parental parents arecultivated and allowed to grow until the time of flowering.Advantageously, during this growth stage, plants are in general treatedwith fertilizer and/or other agricultural chemicals as consideredappropriate by the grower.

At the time of flowering, in the event that plant GMLEA, 6F905, 91CSV-1,91DFA-5, WDAQ2, WDDQ1, 85DGD1, PHEI4, 01ASB1, 01IBH2, NL054B or FBMU, isemployed as the male parent, the tassels of the other parental plant areremoved from all plants employed as the female parental plant. Thedetasseling can be achieved manually but also can be done by machine ifdesired.

The plants are then allowed to continue to grow and naturalcross-pollination occurs as a result of the action of wind, which isnormal in the pollination of grasses, including corn. As a result of theemasculation of the female parent plant, all the pollen from the maleparent plant, e.g., GMLEA, 6F905, 91CSV-1, 91DFA-5, WDAQ2, WDDQ1,85DGD1, PHEI4, 01ASB1, 01IBH2, NL054B or FBMU, is available forpollination because tassels, and thereby pollen bearing flowering parts,have been previously removed from all plants of the inbred plant beingused as the female in the hybridization. Of course, during thishybridization procedure, the parental varieties are grown such that theyare isolated from other corn fields to prevent any accidentalcontamination of pollen from foreign sources. These isolation techniquesare well within the skill of those skilled in this art.

Both of the parent inbred plants of corn are allowed to continue to growuntil maturity, but only the ears from the female inbred parental plantsare harvested to obtain seeds of a novel F₁ hybrid. The novel F₁ hybridseed produced can then be planted in a subsequent growing season withthe desirable characteristics in terms of F₁ hybrid corn plantsproviding improved grain yields and the other desirable characteristicsdisclosed herein, being achieved.

Alternatively, in another embodiment, both first and second parent cornplants can come from the same inbred corn plant, i.e., from the inbredsdesignated, separately, GMLEA, 6F905, 91CSV-1, 91DFA-5, WDAQ2, WDDQ1,85DGD1, PHEI4, 01ASB1, 01IBH2, NL054B or FBMU. Thus, any corn plantproduced using a process of the present invention and inbred corn plantGMLEA, 6F905, 91CSV-1, 91DFA-5, WDAQ2, WDDQ1, 85DGD1, PHEI4, 01ASB1,01IBH2, NL054B or FBMU, is contemplated by this invention. As usedherein, crossing can mean selfing, backcrossing, crossing to another orthe same inbred, crossing to populations, and the like. All corn plantsproduced using the present inbred corn plants, i.e., GMLEA, 6F905,91CSV-1, 91DFA-5, WDAQ2, WDDQ1, 85DGD1, PHEI4, 01ASB1, 01IBH2, NL054B orFBMU, as a parent are within the scope of this invention.

The utility of the inbred plants GMLEA, 6F905, 91CSV-1, 91DFA-5, WDAQ2,WDDQ1, 85DGD1, PHEI4, 01ASB1, 01IBH2, NL054B and FBMU, also extends tocrosses with other species. Commonly, suitable species will be of thefamily Graminaceae, and especially of the genera Zea, Tripsacum, Coix,Schlerachne, Polytoca, Chionachne, and Trilobachne, of the tribeMaydeae. Of these, Zea and Tripsacum, are most preferred. Potentiallysuitable for crosses with GMLEA, 6F905, 91CSV-1, 91DFA-5, WDAQ2, WDDQ1,85DGD1, PHEI4, 01ASB1, 01IBH2, NL054B and FBMU can be the variousvarieties of grain sorghum, Sorghum bicolor (L.) Moench.

A. F₁ Hybrid Corn Plant and Seed Production

Where the inbred corn plant GMLEA, 6F905, 91CSV-1, 91DFA-5, WDAQ2,WDDQ1, 85DGD1, PHEI4, 01ASB1, 01IBH2, NL054B or FBMU is crossed withanother, different, corn inbred, a first generation (F₁) corn hybridplant is produced. Both a F₁ hybrid corn plant and a seed of that F₁hybrid corn plant are contemplated as aspects of the present invention.

Inbreds GMLEA and 6F905 have been used to prepare an F₁ hybrid cornplant, designated DK706; inbreds 91CSV-1 and 01ASB1 have been used toprepare an F₁ hybrid corn plant, designated DK446; inbreds WDAQ2 and01IBH2 have been used to prepare an F₁ hybrid corn plant, designatedDK604; inbreds 01IBH2 and FBMU have been used to prepare an F₁ hybridcorn plant, designated DK527; inbred 91DFA-5 has been used to prepare anF₁ hybrid corn plant, designated DK474; inbred WDDQ1 has been used toprepare an F₁ hybrid corn plant, designated DK642; inbred 85DGD1 hasbeen used to prepare an F₁ hybrid corn plant, designated DK560; inbredPHEI4 has been used to prepare an F₁ hybrid corn plant, designatedDK566; inbred 01IBH2 has additionally been used to prepare the F₁ hybridcorn plant designated DK442; and inbred NL054B has been used to preparean F₁ hybrid corn plant, designated DK626.

The goal of a process of producing an F₁ hybrid is to manipulate thegenetic complement of corn to generate new combinations of genes whichinteract to yield new or improved traits (phenotypic characteristics). Aprocess of producing an F₁ hybrid typically begins with the productionof one or more inbred plants. Those plants are produced by repeatedcrossing of ancestrally related corn plants to try and concentratecertain genes within the inbred plants. The production of inbreds GMLEA,6F905, 91CSV-1, 91DFA-5, WDAQ2, WDDQ1, 85DGD1, PHEI4, 01ASB1, 01IBH2,NL054B and FBMU has been set forth hereinbefore.

Corn has a diploid phase which means two conditions of a gene (twoalleles) occupy each locus (position on a chromosome). If the allelesare the same at a locus, there is said to be homozygosity. If they aredifferent, there is said to be heterozygosity. In a completely inbredplant, all loci are homozygous. Because many loci when homozygous aredeleterious to the plant, in particular leading to reduced vigor, lesskernels, weak and/or poor growth, production of inbred plants is anunpredictable and arduous process. Under some conditions, heterozygousadvantage at some loci effectively bars perpetuation of homozygosity.

Inbreeding requires coddling and sophisticated manipulation by humanbreeders. Even in the extremely unlikely event inbreeding rather thancrossbreeding occurred in natural corn, achievement of completeinbreeding cannot be expected in nature due to well known deleteriouseffects of homozygosity and the large number of generations the plantwould have to breed in isolation. The reason for the breeder to createinbred plants is to have a known reservoir of genes whose gametictransmission is at least somewhat predictable.

The development of inbred plants generally requires at least about 5 to7 generations of selfing. Inbred plants are then cross-bred in anattempt to develop improved F₁ hybrids. Hybrids are then screened andevaluated in small scale field trials. Typically, about 10 to 15phenotypic traits, selected for their potential commercial value, aremeasured. A selection index of the most commercially important traits isused to help evaluate hybrids. FACT, an acronym for Field AnalysisComparison Trial (strip trials), is an on-farm testing program employedby DEKALB Plant Genetics to perform the final evaluation of thecommercial potential of a product.

During the next several years, a progressive elimination of hybridsoccurs based on more detailed evaluation of their phenotype. Eventually,strip trials (FACT) are conducted to formally compare the experimentalhybrids being developed with other hybrids, some of which werepreviously developed and generally are commercially successful. That is,comparisons of experimental hybrids are made to competitive hybrids todetermine if there was any advantage to further commercial developmentof the experimental hybrids. Examples of such comparisons are presentedin XVII, Section B, hereinbelow.

When the inbred parental plant, i.e., GMLEA, 6F905, 91CSV-1, 91DFA-5,WDAQ2, WDDQ1, 85DGD1, PHEI4, 01ASB1, 01IBH2, NL054B or FBMU, is crossedwith another inbred plant to yield a hybrid (such as the hybrids DK706,DK446, DK474, DK642, DK604, DK560, DK566, DK442, DK527, DK626), theoriginal inbred can serve as either the maternal or paternal plant. Formany crosses, the outcome is the same regardless of the assigned sex ofthe parental plants.

However, there is often one of the parental plants that is preferred asthe maternal plant because of increased seed yield and productioncharacteristics. Some plants produce tighter ear husks leading to moreloss, for example due to rot. There can be delays in silk formationwhich deleteriously affect timing of the reproductive cycle for a pairof parental inbreds. Seed coat characteristics can be preferable in oneplant. Pollen can be shed better by one plant. Other variables can alsoaffect preferred sexual assignment of a particular cross.

Table 31 shows the male and female parentage of the exemplary hybridsDK706, DK446, DK474, DK642, DK604, DK560, DK566, DK442, DK527, DK626.

TABLE 31 HYBRID PARENTAGE HYBRID PARENTAGE: HYBRID NAME FEMALE MALEDK706 6F905 GMLEA DK446 01ASB1 91CSV-1 DK474 91DFA-5 78551S DK642 WDDQ1MM402A DK604 01IBH2 WDAQ2 DK560 85DGD1 MBZA DK566 2FADB PHEI4 DK44201IBH2 91BMA2 DK527 FBMU 01IBH2 DK626 NL054B MM402A

B. F₁ Hybrid Comparisons

As mentioned in Section A, hybrids are progressively eliminatedfollowing detailed evaluations of their phenotype, including formalcomparisons with other commercially successful hybrids. Strip trials areused to compare the phenotypes of hybrids grown in as many environmentsas possible. They are performed in many environments to assess overallperformance of the new hybrids and to select optimum growing conditions.Because the corn is grown in close proximity, environmental factors thataffect gene expression, such as moisture, temperature, sunlight andpests, are minimized. For a decision to be made that a hybrid is worthmaking commercially available, it is not necessary that the hybrid bebetter than all other hybrids. Rather, significant improvements must beshown in at least some traits that would create improvements in someniches.

Examples of such comparative data are set forth hereinbelow in Tables 32through 41, which present a comparison of performance data for thehybrids DK706, DK446, DK474, DK642, DK604, DK560, DK566, DK442, DK527and DK626, versus several other selected hybrids of commercial value.

Specifically, Table 32 presents a comparison of performance data forDK706 versus two selected hybrids of commercial value (DK711 and DK715).DK706 has GMLEA and 6F905, both inbreds of the invention, as parents.

Table 33 presents a comparison of performance data for DK446 versus twoselected hybrids of commercial value (DK421 and DK451). DK446 has91CSV-1 and 01ASB1, both inbreds of the invention, as parents.

Table 34 presents a comparison of performance data for DK604 versus twoselected hybrids of commercial value (DK591 and DK612). DK604 has WDAQ2and 01IBH2, both inbreds of the invention, as parents.

Table 35 presents a comparison of performance data for DK527 versus twoselected hybrids of commercial value (DK501 and DK512). DK527 has 01IBH2and FBMU, both inbreds of the invention, as parents.

Table 36 presents a comparison of performance data for DK442 versusseveral selected hybrids of commercial value (DK421, DK446 and DK451).DK442 also has 01IBH2 as one inbred parent.

Table 37 presents a comparison of performance data for DK474 versus twoselected hybrids of commercial value (DK473 and DK485). DK474 has91DFA-5 as one inbred parent.

Table 38 presents a comparison of performance data for DK642 versus twoselected hybrids of commercial value (DK636 and DK646). DK642 has WDDQ1as one inbred parent.

Table 39 presents a comparison of performance data for DK560 versus twoselected hybrids of commercial value (DK554 and DK564). DK560 has 85DGD1as one inbred parent.

Table 40 presents a comparison of performance data for DK566 versus twoselected hybrids of commercial value (DK554 and DK564). DK566 has PHEI4as one inbred parent.

Table 41 presents a comparison of performance data for DK626 versus twoselected hybrids of commercial value (DK623 and DK636). These datarepresent results across years and locations for strip trials. DK626 hasNL054B as one inbred parent.

All the data in Tables 32 through 41 represent results across years andlocations for strip trials. In all cases, the “NTEST” represents thenumber of paired observations in designated tests at locations aroundthe United States.

TABLE 32 COMPARATIVE DATA FOR DK706 N- SI YLD MST STL RTL DRP FLSTD SVHYBRID TEST % C BU PTS % % % % M RAT DK706 F 35 103.2 99.6 13.1 0.3 0.00.0 99.4 DK711 100.7 97.7 13.2 0.9 0.0 0.0 100.7 DK706 R 135 108.4 160.319.3 1.7 0.5 0.0 100.2 6.4 DK715 99.5** 150.6** 19.1* 2.4 0.2* 0.1+100.3 6.4 F 86 104.1 118.5 16.0 1.6 0.1 0.0 99.9 99.4** 113.7** 16.0 1.50.1 0.0 100.0 N- ELSTD PHT EHT BAR SG TST ESTR HYBRID TEST % M INCH INCH% RAT LBS FGDU DAYS DK706 F 35 58.2 119.6 DK711 60.2** 119.7 DK706 R 135102.6 99.3 50.2 0.9 5.1 58.6 1351  120.9 DK715 100.0* 95.3** 43.3** 0.65.0 57.4** 1314** 120.6+ F 86 58.2 119.9 57.3** 119.6 LegendAbbreviations SI % C = Selection Index (Percent of Check) YLD BU = Yield(Bushels/Acre) MST PTS = Moisture STL % = Stalk Lodging (Percent) RTL %= Root Lodging (Percent) DRP % = Dropped Ears (Percent) FLSTD % M =Final Stand (Percent of Test Mean) SV RAT = Seedling Vigor Rating ELSTD% M = Early Stand (Percent of Test Mean) PHT INCH = Plant Height(Inches) EHT INCH = Ear Height (Inches) BAR % = Barren Plants (Percent)SG RAT = Staygreen Rating TST LBS = Test Weight (Pounds) FGDU = GDUs toShed ESTR DAYS = Estimated Relative Maturity (Days) Significance levelsare indicated as: +10 percent *5 percent **1 percent

TABLE 33 COMPARATIVE DATA FOR DK446 N- SI YLD MST STL RTL DRP FLSTD SVHYBRID TEST % C BU PTS % % % % M RAT DK446 R 95 107.5 139.1 22.2 2.5 1.40.2 100.3 5.9 DK421 97.5** 126.8** 21.3** 2.8 0.5* 0.1 100.2 5.1** F 87100.2 109.2 24.3 3.7 1.7 0.0 100.0 99.4 103.9** 23.0** 2.2 0.1** 0.099.8 DK446 R 62 109.7 148.4 22.5 2.7 1.3 0.2 100.3 5.8 DK451 96.2**136.6** 23.7** 3.2 0.2* 0.1 100.4 5.6 F 54 103.4 126.2 22.4 1.2 2.0 0.1100.7 98.4** 125.0 24.5** 1.8 0.1* 0.0 101.6 N- ELSTD PHT EHT BAR SG TSTESTR HYBRID TEST % M INCH INCH % RAT LBS FGDU DAYS DK446 R 95 103.0 89.543.4 0.0 5.5 51.7 1274 94.2 DK421 100.3* 84.4** 41.4** 0.0 4.3** 53.81280 93.5** F 87 51.1 93.5 51.2 92.5** DK446 R 62 103.5 90.5 43.0 0.05.5 58.4 1272 94.3 DK451 100.1** 82.4** 35.3** 0.0 4.7+ 56.2  1253*95.3** F 54 51.9 94.1 50.6** 96.6** Legend Abbreviations SI % C =Selection Index (Percent of Check) YLD BU = Yield (Bushels/Acre) MST PTS= Moisture STL % = Stalk Lodging (Percent) RTL % = Root Lodging(Percent) DRP % = Dropped Ears (Percent) FLSTD % M = Final Stand(Percent of Test Mean) SV RAT = Seedling Vigor Rating ELSTD % M = EarlyStand (Percent of Test Mean) PHT INCH = Plant Height (Inches) EHT INCH =Ear Height (Inches) BAR % = Barren Plants (Percent) SG RAT = StaygreenRating TST LBS = Test Weight (Pounds) FGDU = GDUs to Shed ESTR DAYS =Estimated Relative Maturity (Days) Significance levels are indicated as:+10 percent *5 percent **1 percent

TABLE 34 COMPARATIVE DATA FOR DK604 N- SI YLD MST STL RTL DRP FLSTD SVHYBRID TEST % C BU PTS % % % % M RAT DK604 R 395 107.6 149.5 19.9 3.91.6 0.3 99.8 6.1 DK591 102.2** 145.4** 20.7** 4.2+ 1.1** 0.2+ 100.3**5.7** F 130 107.3 141.6 21.5 3.1 0.5 0.4 98.8 102.9** 139.4* 22.1**4.5** 0.6 0.3 101.2** DK604 R 56 108.2 172.6 19.9 2.6 2.1 0.2 99.8 6.1DK612 77.8** 145.2** 22.1** 2.7 2.2 0.1 100.0 5.9 F 26 108.9 132.9 20.65.2 0.9 1.0 100.0 90.6** 117.4** 21.7* 6.1 1.1 0.8 100.9 N- ELSTD PHTEHT BAR SG TST ESTR HYBRID TEST % M INCH INCH % RAT LBS FGDU DAYS DK604R 395 98.0 91.7 45.8 0.8 5.9 52.3 1356  108.0 DK591 99.9** 92.5* 44.8*0.8 5.0** 52.8* 1343** 108.8** F 130 52.8 107.9 52.5** 108.9** DK604 R56 98.3 91.4 45.6 0.9 5.9 54.4 1325  108.5 DK612 96.3+ 82.3** 35.8** 1.43.5** 57.5** 1255** 110.8** F 26 53.5 107.8 54.7** 110.6** LegendAbbreviations SI % C = Selection Index (Percent of Check) YLD BU = Yield(Bushels/Acre) MST PTS = Moisture STL % = Stalk Lodging (Percent) RTL %= Root Lodging (Percent) DRP % = Dropped Ears (Percent) FLSTD % M =Final Stand (Percent of Test Mean) SV RAT = Seedling Vigor Rating ELSTD% M = Early Stand (Percent of Test Mean) PHT INCH = Plant Height(Inches) EHT INCH = Ear Height (Inches) BAR % = Barren Plants (Percent)SG RAT = Staygreen Rating TST LBS = Test Weight (Pounds) FGDU = GDUs toShed ESTR DAYS = Estimated Relative Maturity (Days) Significance levelsare indicated as: +10 percent *percent **1 percent

TABLE 35 COMPARATIVE DATA FOR DK527 N- SI YLD MST STL RTL DRP FLSTD SVHYBRID TEST % C BU PTS % % % % M RAT DK527 R 146 103.4 152.8 22.7 3.62.5 0.2 99.8 5.5 DK501 100.4* 147.2** 22.6 2.2** 0.7** 0.1 100.3* 5.1**F 58 107.7 121.1 23.7 1.7 1.6 0.0 98.7 102.2** 117.3* 25.0** 1.1* 1.00.0 99.2 DK527 R 262 98.6 134.7 24.0 3.3 2.6 0.1 100.3 5.7 DK512 98.0130.9** 23.2** 3.1 0.9** 0.2 99.9 4.9** F 153 105.0 123.6 22.9 2.6 1.70.1 99.5 102.2** 120.0** 22.7 3.1 0.6** 0.1 100.7 N- ELSTD PHT EHT BARSG TST ESTR HYBRID TEST % M INCH INCH % RAT LBS FGDU DAYS DK527 R 14698.5 87.2 39.5 0.5 4.9 51.9 1283 100.3 DK501 99.9 82.8** 34.0** 1.1+4.3** 50.8 1276 100.3 F 58 50.7 99.3 48.9** 100.4** DK527 R 262 100.585.5 38.4 0.4 4.7 50.6 1255 101.2 DK512 99.7 87.9** 42.2** 0.4 4.648.4**  1265* 100.2** F 153 51.8 100.4 49.9** 99.9* Legend AbbreviationsSI % C = Selection Index (Percent of Check) YLD BU = Yield(Bushels/Acre) MST PTS = Moisture STL % = Stalk Lodging (Percent) RTL %= Root Lodging (Percent) DRP % = Dropped Ears (Percent) FLSTD % M =Final Stand (Percent of Test Mean) SV RAT = Seedling Vigor Rating ELSTD% M = Early Stand (Percent of Test Mean) PHT INCH = Plant Height(Inches) EHT INCH = Ear Height (Inches) BAR % = Barren Plants (Percent)SG RAT = Staygreen Rating TST LBS = Test Weight (Pounds) FGDU = GDUs toShed ESTR DAYS = Estimated Relative Maturity (Days) Significance levelsare indicated as: +10 percent *5 percent **1 percent

TABLE 36 COMPARATIVE DATA FOR DK442 N- SI YLD MST STL RTL DRP FLSTD SVHYBRID TEST % C BU PTS % % % % M RAT DK442 R 96 106.0 138.9 21.5 5.3 0.70.1 100.1 6.0 DK421 97.7** 128.0** 21.2 2.8** 0.5+ 0.1 100.3 5.2** F 81101.2 112.0 25.0 6.4 1.0 0.1 100.6 100.1 104.7** 23.4** 2.4** 0.1 0.0100.2 DK442 R 155 102.7 129.2 22.8 4.9 1.4 0.0 100.4 5.8 DK446 105.7**131.4* 22.8 2.5** 2.4** 0.0 100.3 5.9 F 92 102.5 114.3 23.8 5.7 1.2 0.0101.0 100.6 111.6* 23.9 3.7** 1.9 0.0 100.6 DK442 R 63 108.1 147.4 21.55.5 0.2 0.2 100.2 6.1 DK451 96.3** 138.3** 23.6** 3.4** 0.2 0.1 100.35.7** F 44 103.2 122.1 23.9 2.5 0.4 0.1 101.0 97.6** 120.4 26.0** 2.30.1+ 0.0 101.6 N- ELSTD PHT EHT BAR SG TST ESTR HYBRID TEST % M INCHINCH % RAT LBS FGDU DAYS DK442 R 96 102.3 87.2 43.5 0.0 5.9 51.3 1297 93.6 DK421 100.7 84.2** 41.1** 0.0 4.3** 53.8+ 1283*  93.5 F 81 49.793.5 51.0** 92.3** DK442 R 155 105.3 86.4 44.1 6.1 51.1 1269  94.6 DK446102.7** 88.4** 43.9 5.1** 51.7 1247** 94.6 F 92 50.1 93.8 51.4** 93.6DK442 R 63 102.4 88.2 43.3 0.0 5.7 56.3 1298  93.4 DK451 100.4 81.9**35.8** 0.0 4.6+ 56.2 1255** 95.3** F 44 50.1 93.8 50.2 96.1** LegendAbbreviations SI % C = Selection Index (Percent of Check) YLD BU = Yield(Bushels/Acre) MST PTS = Moisture STL % = Stalk Lodging (Percent) RTL %= Root Lodging (Percent) DRP % = Dropped Ears (Percent) FLSTD % M =Final Stand (Percent of Test Mean) SV RAT = Seedling Vigor Rating ELSTD% M = Early Stand (Percent of Test Mean) PHT INCH = Plant Height(Inches) EHT INCH = Ear Height (Inches) BAR % = Barren Plants (Percent)SG RAT = Staygreen Rating TST LBS = Test Weight (Pounds) FGDU = GDUs toShed ESTR DAYS = Estimated Relative Maturity (Days) Significance levelsare indicated as: +10 percent *5 percent **1 percent

TABLE 37 COMPARATIVE DATA FOR DK474 N- SI YLD MST STL RTL DRP FLSTD SVHYBRID TEST % C BU PTS % % % % M RAT DK474 R 83 104.1 150.1 23.9 3.0 0.70.0 100.1 5.7 DK473 92.7** 142.4** 25.1** 4.8** 0.4 0.0 99.7 5.1** F 26103.2 140.0 27.9 3.7 0.9 0.0 99.5 98.0* 136.4 29.3** 2.8 0.4 0.0 100.2DK474 R 364 104.5 142.1 24.0 3.0 0.8 0.1 100.2 5.6 DK485 98.9** 140.7*25.9** 3.7** 1.0 0.1 99.9 5.6 F 153 103.6 131.4 25.2 2.2 0.4 0.0 99.496.4** 129.2* 27.7** 2.1 0.5 0.0 98.5 N- ELSTD PHT EHT BAR SG TST ESTRHYBRID TEST % M INCH INCH % RAT LBS FGDU DAYS DK474 R 83 99.3 88.5 39.30.3 5.8 55.3 1236  96.6 DK473 100.4 87.6+ 39.5 0.0 5.6 54.6** 1247+ 97.8** F 26 51.5 95.9 51.2 97.6** DK474 R 364 97.8 86.4 39.5 0.2 6.254.5 1267  95.8 DK485 98.7+ 86.0+ 41.2** 0.0 6.1 53.0** 1278** 97.1** F153 50.9 95.8 49.6** 98.3** Legend Abbreviations SI % C = SelectionIndex (Percent of Check) YLD BU = Yield (Bushels/Acre) MST PTS =Moisture STL % = Stalk Lodging (Percent) RTL % = Root Lodging (Percent)DRP % = Dropped Ears (Percent) FLSTD % M = Final Stand (Percent of TestMean) SV RAT = Seedling Vigor Rating ELSTD % M = Early Stand (Percent ofTest Mean) PHT INCH = Plant Height (Inches) EHT INCH = Ear Height(Inches) BAR % = Barren Plants (Percent) SG RAT = Staygreen Rating TSTLBS = Test Weight (Pounds) FGDU = GDUs to Shed ESTR DAYS = EstimatedRelative Maturity (Days) Significance levels are indicated as: +10percent *5 percent **1 percent

TABLE 38 COMPARATIVE DATA FOR DK642 N- SI YLD MST STL RTL DRP FLSTD SVHYBRID TEST % C BU PTS % % % % M RAT DK642 R 85 98.7 172.7 19.5 2.1 2.00.0 100.3 6.2 DK636 88.4** 163.2** 19.6 2.1 3.5+ 0.0 99.8* 5.0** F 24108.4 157.4 21.2 3.4 0.3 0.3 100.2 95.1** 144.2** 21.2 2.3 1.6 0.2 102.1DK642 R 339 104.9 164.6 19.1 2.5 0.8 0.1 100.4 6.7 DK646 97.9** 159.4**19.5** 3.4** 1.1 0.1 100.3 6.3** F 82 103.2 144.9 20.1 3.6 0.3 0.2 101.097.7** 139.9** 20.6** 2.7 0.5 0.1+ 99.6 N- ELSTD PHT EHT BAR SG TST ESTRHYBRID TEST % M INCH INCH % RAT LBS FGDU DAYS DK642 R 85 102.4 98.5 39.91.5 5.5 56.8 1404  114.3 DK636 96.0** 89.8** 42.0* 1.0 2.9** 59.8**1367** 114.6+ F 24 53.4 112.9 55.8** 113.3 DK642 R 339 103.3 99.4 40.31.5 5.3 56.0 1387  113.9 DK646 101.5** 100.2 41.8** 1.3 4.7* 56.6**1353** 114.6** F 82 54.4 112.8 54.8** 113.8** Legend Abbreviations SI %C = Selection Index (Percent of Check) YLD BU = Yield (Bushels/Acre) MSTPTS = Moisture STL % = Stalk Lodging (Percent) RTL % = Root Lodging(Percent) DRP % = Dropped Ears (Percent) FLSTD % M = Final Stand(Percent of Test Mean) SV RAT = Seedling Vigor Rating ELSTD % M = EarlyStand (Percent of Test Mean) PHT INCH = Plant Height (Inches) EHT INCH =Ear Height (Inches) BAR % = Barren Plants (Percent) SG RAT = StaygreenRating TST LBS = Test Weight (Pounds) FGDU = GDUs to Shed ESTR DAYS =Estimated Relative Maturity (Days) Significance levels are indicated as:+10 percent *5 percent **1 percent

TABLE 39 COMPARATIVE DATA FOR DK560 N- SI YLD MST STL RTL DRP FLSTD SVHYBRID TEST % C BU PTS % % % % M RAT DK560 R 396 107.6 145.8 20.5 4.01.9 0.1 100.3 5.6 DK554 99.7** 135.9** 19.8** 4.3 0.7** 0.1 100.2 5.5 F154 104.8 128.4 21.6 4.0 1.7 0.3 101.8 101.3** 123.6** 21.8 2.8** 0.4*0.3 99.3** DK560 R 233 108.8 154.6 20.4 4.4 1.9 0.2 100.2 5.5 DK564102.7** 149.7** 20.8** 3.7* 2.9 0.2 100.3 5.2** F 112 106.4 127.5 20.94.4 2.0 0.4 101.5 102.2** 124.3** 21.9** 3.2* 1.6 0.5 98.9* N- ELSTD PHTEHT BAR SG TST ESTR HYBRID TEST % M INCH INCH % RAT LBS FGDU DAYS DK560R 396 101.0 88.9 42.6 0.8 3.9 52.9 1367  106.0 DK554 100.3 86.7** 41.0**0.7 3.7* 50.1** 1314** 105.4** F 154 53.1 105.2 51.5** 105.3 DK560 R 233100.6 88.9 42.4 0.7 4.2 54.4 1343  106.1 DK564 100.1 90.9** 35.4** 1.7**3.9* 52.6** 1307** 106.3** F 112 53.4 105.2 52.2** 106.1** LegendAbbreviations SI % C = Selection Index (Percent of Check) YLD BU = Yield(Bushels/Acre) MST PTS = Moisture STL % = Stalk Lodging (Percent) RTL %= Root Lodging (Percent) DRP % = Dropped Ears (Percent) FLSTD % M =Final Stand (Percent of Test Mean) SV RAT = Seedling Vigor Rating ELSTD% M = Early Stand (Percent of Test Mean) PHT INCH = Plant Height(Inches) EHT INCH = Ear Height (Inches) BAR % = Barren Plants (Percent)SG RAT = Staygreen Rating TST LBS = Test Weight (Pounds) FGDU = GDUs toShed ESTR DAYS = Estimated Relative Maturity (Days) Significance levelsare indicated as: +10 percent *5 percent **1 percent

TABLE 40 COMPARATIVE DATA FOR DK566 N- SI YLD MST STL RTL DRP FLSTD SVHYBRID TEST % C BU PTS % % % % M RAT DK566 R 285 108.7 144.7 20.9 3.32.1 0.1 100.5 5.8 DK554 100.4** 133.7** 20.1** 3.3 0.7** 0.2 100.1* 5.7+F 104 105.2 124.7 23.3 4.6 1.6 0.3 99.3 99.8** 118.0** 23.5 3.5 0.5* 0.2100.7 DK566 R 149 108.7 151.2 20.9 3.3 2.2 0.1 100.5 5.6 DK564 101.7**145.8** 21.4** 2.9+ 3.4 0.2 100.0+ 5.1** F 76 108.5 121.7 21.6 3.7 1.20.4 99.3 101.0** 115.6** 22.5** 3.1 1.4 0.3 99.1 N- ELSTD PHT EHT BAR SGTST ESTR HYBRID TEST % M INCH INCH % RAT LBS FGDU DAYS DK566 R 285 101.888.4 41.6 0.6 3.9 50.9 1335  105.8 DK554 100.3** 86.6** 40.8* 0.6 3.849.0** 1319** 105.2** F 104 52.0 105.1 50.6** 105.3 DK566 R 149 101.987.9 40.7 0.5 4.3 51.9 1320  105.6 DK564 99.8** 90.5** 35.5** 0.9 4.151.1+ 1316  106.0 F 76 52.5 105.3 51.7** 106.4** Legend Abbreviations SI% C = Selection Index (Percent of Check) YLD BU = Yield (Bushels/Acre)MST PTS = Moisture STL % = Stalk Lodging (Percent) RTL % = Root Lodging(Percent) DRP % = Dropped Ears (Percent) FLSTD %M = Final Stand (Percentof Test Mean) SV RAT = Seedling Vigor Rating ELSTD %M = Early Stand(Percent of Test Mean) PHT INCH = Plant Height (Inches) EHT INCH = EarHeight (Inches) BAR % = Barren Plants (Percent) SG RAT = StaygreenRating TST LBS = Test Weight (Pounds) FGDU = GDUs to Shed ESTR DAYS =Estimated Relative Maturity (Days) Significance levels are indicated as:+10 percent *5 percent **1 percent

TABLE 40 COMPARATIVE DATA FOR DK626 N- SI YLD MST STL RTL DRP FLSTD SVHYBRID TEST % C BU PTS % % % % M RAT DK626 R 195 106.3 165.8 19.7 3.72.4 0.4 100.1 6.2 DK623 93.1** 150.8** 19.9* 3.1+ 0.6** 0.1** 100.05.3** F 81 108.7 147.3 19.6 4.1 1.4 0.8 100.2 99.7** 135.8** 19.1** 3.40.4* 0.5+ 100.0 DK626 R 91 104.8 176.5 18.0 3.8 3.9 0.0 100.0 6.1 DK63688.5** 160.9** 19.4** 2.1 3.4 0.0 99.8 5.0** F 42 109.3 130.6 18.6 5.11.7 0.7 99.8 94.0** 116.8** 20.0** 3.5* 1.6 0.5 100.4 N- ELSTD PHT EHTBAR SG TST ESTR HYBRID TEST % M INCH INCH % RAT LBS FGDU DAYS DK626 R195 101.9 102.0 47.4 1.6 4.8 54.9 1360  111.0 DK623 98.0** 91.3** 39.4**1.4 3.6** 55.5** 1321** 111.5** F 81 54.6 110.9 55.3** 110.4 DK626 R 91100.2 101.4 45.2 1.5 4.2 57.8 1402  112.4 DK636 96.0** 89.9** 42.1** 1.02.9** 59.8** 1367** 114.4 F 42 55.0 110.9 56.8** 113.3** LegendAbbreviations SI % C = Selection Index (Percent of Check) YLD BU = Yield(Bushels/Acre) MST PTS = Moisture STL % = Stalk Lodging (Percent) RTL %= Root Lodging (Percent) DRP % = Dropped Ears (Percent) FLSTD %M = FinalStand (Percent of Test Mean) SV RAT = Seedling Vigor Rating ELSTD %M =Early Stand (Percent of Test Mean) PHT INCH = Plant Height (Inches) EHTINCH = Ear Height (Inches) BAR % = Barren Plants (Percent) SG RAT =Staygreen Rating TST LBS = Test Weight (Pounds) FGDU = GDUs to Shed ESTRDAYS = Estimated Relative Maturity (Days) Significance levels areindicated as: + = 10 percent * = 5 percent ** = 1 percent

As can be seen in Table 32 through Table 41, each of the hybrids DK706,DK446, DK474, DK642, DK604, DK560, DK566, DK442, DK527 and DK626 havesignificantly higher yield when compared to several successfulcommercial hybrids. Significant differences are also shown in Tables 32through 41 for many other traits.

C. Physical Description of F₁ Hybrids

The present invention also provides F₁ hybrid corn plants derived fromthe corn plants GMLEA, 6F905, 91CSV-1, 91DFA-5, WDAQ2, WDDQ1, 85DGD1,PHEI4, 01ASB1, 01IBH2, NL054B and FBMU. Physical characteristics ofexemplary hybrids are set forth in Tables 42 through 51. An explanationof terms used in Tables 42 through 51 can be found in the Definitions,set forth hereinabove.

Table 42 concerns DK706, which has GMLEA and 6F905, both inbreds of theinvention, as parents. Table 43 concerns DK446, which has 91CSV-1 and01ASB1, both inbreds of the invention, as parents. Table 44 concernsDK604, which has WDAQ2 and 01IBH2, both inbreds of the invention, asparents. Table 45 concerns DK527, which has 01IBH2 and FBMU, bothinbreds of the invention, as parents. Table 46 concerns DK442, whichalso has 01IBH2 as one inbred parent.

Table 47 concerns DK474, which has 91DFA-5 as one inbred parent. Table48 concerns DK642, which has WDDQ1 as a parent. Table 49 concerns DK560,which has 85DGD1 as one inbred parent. Table 50 concerns DK566, whichhas PHEI4 as a parent. Table 51 concerns DK626, which has NL054B as oneinbred parent.

TABLE 42 MORPHOLOGICAL TRAITS FOR THE DK706 PHENOTYPE YEAR OF DATA:1992, 1993 CHARACTERISTIC VALUE* 1. Stalk Diameter (Width) cm.  2.9Nodes With Brace Roots  2.1 Brace Root Color GREEN Internode DirectionZIGZAG Internode Length cm. 17.6 2. Leaf Angle INTERMEDIATE Number 19.9Color DARK GREEN Length cm. 95.2 Width cm. 11.4 Marginal Waves FEWLongitudinal Creases ABSENT 3. Tassel Length cm. 44.6 Spike Length cm.23.8 Peduncle Length cm.  9.7 Attitude OPEN Branch Angle INTERMEDIATEBranch Number 10.2 Anther Color RED Glume Color GREEN Glume Band ABSENT4. Ear Silk Color PINK Number Per Stalk  1.0 Position (Attitude) UPRIGHTLength cm. 18.2 Shape SEMI-CONICAL Diameter cm. 46.0 Weight gm. 229.2 Shank Length cm. 10.8 Shank Internode  8.3 Husk Bract SHORT Husk Covercm.  2.2 Husk Opening INTERMEDIATE Husk Color Fresh GREEN Husk Color DryBUFF Cob Diameter cm. 26.5 Cob Color RED Shelling Percent 88.5 5. KernelRow Number 17.0 Number Per Row 44.5 Row Direction CURVED Type DENT CapColor YELLOW Side Color ORANGE Length (Depth) mm. 12.7 Width mm.  8.0Thickness  4.0 Weight of 1000K gm. 287.5  Endosperm Type NORMALEndosperm Color YELLOW *These are typical values. Values may vary due toenvironment. Other values that are substantially equivalent are alsowithin the scope of the invention. Substantially equivalent refers toquantitative traits that when compared do not show statisticaldifferences of their means.

TABLE 43 MORPHOLOGICAL TRAITS FOR THE DK446 PHENOTYPE YEAR OF DATA:1992, 1993 CHARACTERISTIC VALUE* 1. Stalk Diameter (Width) cm.  2.3Anthocyanin ABSENT Nodes With Brace Roots  1.2 Brace Root Color GREENInternode Direction STRAIGHT Internode Length cm. 18.4 2. Leaf AngleINTERMEDIATE Number 20.1 Color DARK GREEN Length cm. 92.4 Width cm.  9.8Marginal Waves MANY Longitudinal Creases FEW 3. Tassel Length cm. 38.4Spike Length cm. 29.1 Peduncle Length cm. 10.1 Attitude OPEN BranchAngle INTERMEDIATE Branch Number  8.5 Anther Color GREEN-YELLOW GlumeBand ABSENT 4. Ear Number Per Stalk  1.0 Length cm. 19.9 ShapeSEMI-CONICAL Diameter cm. 43.5 Weight gm. 186.9  Shank Length cm. 12.6Shank Internode  5.5 Husk Bract SHORT Husk Cover cm.  3.1 Husk ColorFresh GREEN Husk Color Dry BUFF Cob Diameter cm. 20.6 Cob Color RED CobStrength WEAK Shelling Percent 88.8 5. Kernel Row Number 13.4 Number PerRow 46.7 Row Direction CURVED Type DENT Side Color ORANGE Length (Depth)mm. 11.9 Width mm.  8.7 Thickness  3.9 Weight of 1000K gm. 316.5 Endosperm Type NORMAL Endosperm Color YELLOW *These are typical values.Values may vary due to environment. Other values that are substantiallyequivalent are also within the scope of the invention. Substantiallyequivalent refers to quantitative traits that when compared do not showstatistical differences of their means.

TABLE 44 MORPHOLOGICAL TRAITS FOR THE DK604 PHENOTYPE YEAR OF DATA: 1993CHARACTERISTIC VALUE* 1. Stalk Diameter (Width) cm.  2.5 AnthocyaninABSENT Nodes With Brace Roots  1.2 Brace Root Color GREEN InternodeLength cm.  17.3 2. Leaf Angle UPRIGHT Number  19.8 Color DARK GREENLength cm.  92.3 Width cm.  9.6 Sheath Pubescence HEAVY Marginal WavesFEW Longitudinal Creases FEW 3. Tassel Length cm.  47.2 Spike Length cm. 28.6 Peduncle Length cm.  15.0 Branch Angle UPRIGHT Branch Number  8.9Glume Color GREEN Glume Band ABSENT 4. Ear Silk Color GREEN-YELLOWNumber Per Stalk  1.1 Length cm.  18.1 Shape SEMI-CONICAL Diameter cm. 46.2 Weight gm. 195.9 Shank Length cm.  11.3 Shank Internode  6.5 HuskBract SHORT Husk Cover cm.  0.8 Husk Opening OPEN Husk Color Fresh GREENHusk Color Dry BUFF Cob Diameter cm.  24.0 Cob Color RED ShellingPercent  89.5 5. Kernel Row Number  17.5 Number Per Row  38.7 RowDirection CURVED Type DENT Length (Depth) mm.  13.2 Width mm.  8.1Thickness  4.2 Weight of 1000K gm. 293.3 Endosperm Type NORMAL EndospermColor YELLOW *These are typical values. Values may vary due toenvironment. Other values that are substantially equivalent are alsowithin the scope of the invention. Substantially equivalent refers toquantitative traits that when compared do not show statisticaldifferences of their means.

TABLE 45 MORPHOLOGICAL TRAITS FOR THE DK527 PHENOTYPE YEAR OF DATA:1992, 1993 CHARACTERISTIC VALUE* 1. Stalk Diameter (Width) cm.  2.2Nodes With Brace Roots  1.1 Brace Root Color GREEN Internode DirectionSTRAIGHT Internode Length cm.  18.0 2. Leaf Number  19.6 Color DARKGREEN Length cm.  85.2 Width cm.  9.6 Sheath Pubescence MEDIUMLongitudinal Creases FEW 3. Tassel Length cm.  31.2 Spike Length cm. 22.2 Peduncle Length cm.  13.9 Attitude COMPACT Branch Number  7.9Anther Color RED Glume Color GREEN Glume Band ABSENT 4. Ear Silk ColorGREEN-YELLOW Number Per Stalk  1.0 Length cm.  19.3 Shape SEMI-CONICALDiameter cm.  45.7 Weight gm. 190.4 Shank Length cm.  11.6 ShankInternode  5.6 Husk Bract SHORT Husk Cover cm.  1.1 Husk Opening OPENHusk Color Fresh GREEN Husk Color Dry BUFF Cob Diameter cm.  23.8 CobStrength STRONG Shelling Percent  88.8 5. Kernel Row Number  16.2 NumberPer Row  42.0 Row Direction CURVED Type DENT Length (Depth) mm.  13.3Width mm.  7.9 Thickness  3.9 Weight of 1000K gm. 289.5 Endosperm TypeNORMAL Endosperm Color YELLOW *These are typical values. Values may varydue to environment. Other values that are substantially equivalent arealso within the scope of the invention. Substantially equivalent refersto quantitative traits that when compared do not show statisticaldifferences of their means.

TABLE 46 MORPHOLOGICAL TRAITS FOR THE DK442 PHENOTYPE YEAR OF DATA:1992, 1993 CHARACTERISTIC VALUE* 1. Stalk Diameter (Width) cm.  2.3Nodes With Brace Roots  1.6 Anthocyanin ABSENT Brace Root Color —Internode Direction STRAIGHT Internode Length cm.  16.8 2. Leaf Number 19.6 Angle INTERMEDIATE Color DARK GREEN Length cm.  81.9 Width cm. 10.2 Sheath Anthocyanin — Sheath Pubescence LIGHT Marginal Waves FEWLongitudinal Creases FEW 3. Tassel Length cm.  34.8 Spike Length cm. 27.5 Peduncle Length cm.  9.8 Attitude COMPACT Branch AngleINTERMEDIATE Branch Number  5.8 Anther Color PINK Glume Color GREENGlume Band ABSENT 4. Ear Silk Color GREEN-YELLOW Position UPRIGHT NumberPer Stalk  1.1 Length cm.  18.2 Shape SEMI-CONICAL Diameter cm.  42.5Weight gm. 186.9 Shank Length cm.  11.9 Shank Internode No.  5.9 HuskBract SHORT Husk Cover cm.  1.6 Husk Opening — Husk Color Fresh GREENHusk Color Dry BUFF Cob Diameter cm.  22.0 Cob Color RED Cob Strength —Shelling Percent  89.3 5. Kernel Row Number  14.4 Number Per Row  39.7Row Direction CURVED Type DENT Cap Color YELLOW Side Color ORANGE Length(Depth) mm.  13.7 Width mm.  8.0 Thickness  4.0 Weight of 1000K gm.321.0 Endosperm Type NORMAL Endosperm Color YELLOW *These are typicalvalues. Values may vary due to environment. Other values that aresubstantially equivalent are also within the scope of the invention.Substantially equivalent refers to quantitative traits that whencompared do not show statistical differences of their means.

TABLE 47 MORPHOLOGICAL TRAITS FOR THE DK474 PHENOTYPE YEAR OF DATA:1991, 1992, 1993 CHARACTERISTIC VALUE* 1. Stalk Diameter (Width) cm. 2.6 Nodes With Brace Roots  1.2 Internode Direction STRAIGHT InternodeLength cm.  19.8 2. Leaf Number  18.6 Color DARK GREEN Length cm.  89.0Width cm.  11.0 Marginal Waves MANY Longitudinal Creases FEW 3. TasselLength cm.  39.1 Spike Length cm.  28.8 Peduncle Length cm.  8.3 BranchNumber  6.3 Anther Color GREEN-YELLOW Glume Color GREEN Glume BandABSENT 4. Ear Number Per Stalk  1.0 Position (Attitude) UPRIGHT Lengthcm.  20.1 Shape SEMI-CONICAL Diameter cm.  43.3 Weight gm. 192.5 ShankLength cm.  14.1 Shank Internode  5.2 Husk Bract SHORT Husk Cover cm. 1.8 Husk Color Fresh GREEN Husk Color Dry BUFF Cob Diameter cm.  20.9Cob Color RED Shelling Percent  87.8 5. Kernel Row Number  14.3 NumberPer Row  41.2 Row Direction CURVED Type DENT Side Color ORANGE Length(Depth) mm.  11.8 Width rim.  8.5 Thickness  4.0 Weight of 1000K gm.307.5 Endosperm Type NORMAL Endosperm Color YELLOW *These are typicalvalues. Values may vary due to environment. Other values that aresubstantially equivalent are also within the scope of the invention.Substantially equivalent refers to quantitative traits that whencompared do not show statistical differences of their means.

TABLE 48 MORPHOLOGICAL TRAITS FOR THE DK642 PHENOTYPE YEAR OF DATA:1992, 1993 CHARACTERISTIC VALUE* 1. Stalk Diameter (Width) cm.  2.8Anthocyanin ABSENT Nodes With Brace Roots  1.6 Brace Root Color GREENInternode Direction STRAIGHT Internode Length cm.  17.7 2. Leaf AngleUPRIGHT Number  20.1 Color DARK GREEN Length cm. 107.4 Width cm.  11.9Marginal Waves FEW Longitudinal Creases ABSENT 3. Tassel Length cm. 51.3 Spike Length cm.  30.9 Peduncle Length cm.  11.7 Attitude COMPACTBranch Angle INTERMEDIATE Branch Number  5.5 Anther Color GREEN-YELLOWGlume Color GREEN Glume Band ABSENT 4. Ear Silk Color GREEN-YELLOWNumber Per Stalk  1.0 Length cm.  20.3 Shape SEMI-CONICAL Diameter cm. 46.5 Weight gm. 211.3 Shank Length cm.  13.5 Shank Internode  8.8 HuskBract SHORT Husk Cover cm.  3.2 Husk Opening OPEN Husk Color Fresh GREENHusk Color Dry BUFF Cob Diameter cm.  26.5 Cob Color RED Cob StrengthWEAK Shelling Percent  90.5 5. Kernel Row Number  18.6 Number Per Row 40.3 Row Direction CURVED Type DENT Cap Color YELLOW Side Color ORANGELength (Depth) mm.  13.1 Width mm.  8.0 Thickness  5.0 Weight of 1000Kgm. 318.0 Endosperm Type NORMAL Endosperm Color YELLOW *These aretypical values. Values may vary due to environment. Other values thatare substantially equivalent are also within the scope of the invention.Substantially equivalent refers to quantitative traits that whencompared do not show statistical differences of their means.

TABLE 49 MORPHOLOGICAL TRAITS FOR THE DK560 PHENOTYPE YEAR OF DATA:1992, 1993 CHARACTERISTIC VALUE* 1. Stalk Diameter (Width) cm.  2.3Anthocyanin ABSENT Nodes With Brace Roots  1.3 Brace Root Color GREENInternode Length cm.  17.3 2. Leaf Angle UPRIGHT Number  20.4 Color DARKGREEN Length cm.  93.6 Width cm.  10.4 Marginal Waves FEW 3. TasselLength cm.  36.9 Spike Length cm.  27.0 Peduncle Length cm.  10.1 BranchNumber  8.4 Glume Color GREEN Glume Band ABSENT 4. Ear Silk ColorGREEN-YELLOW Number Per Stalk  1.0 Position (Attitude) UPRIGHT Lengthcm.  18.8 Shape SEMI-CONICAL Diameter cm.  47.3 Weight gm. 190.8 ShankLength cm.  11.3 Shank Internode  6.9 Husk Bract SHORT Husk Cover cm. 3.5 Husk Color Fresh GREEN Husk Color Dry BUFF Cob Diameter cm.  23.7Cob Color RED Cob Strength STRONG Shelling Percent  88.1 5. Kernel RowNumber  17.6 Number Per Row  44.2 Row Direction CURVED Type DENT CapColor YELLOW Length (Depth) mm.  11.5 Width mm.  7.6 Thickness  3.5Weight of 1000K gm. 232.0 Endosperm Type NORMAL Endosperm Color YELLOW*These are typical values. Values may vary due to environment. Othervalues that are substantially equivalent are also within the scope ofthe invention. Substantially equivalent refers to quantitative traitsthat when compared do not show statistical differences of their means.

TABLE 50 MORPHOLOGICAL TRAITS FOR THE DK566 PHENOTYPE YEAR OF DATA:1992, 1993 CHARACTERISTIC VALUE* 1. Stalk Diameter (Width) cm.  2.3Nodes With Brace Roots  1.2 Brace Root Color GREEN Internode Length cm. 14.8 2. Leaf Number  20.3 Length cm.  90.4 Width cm.  9.8 3. TasselLength cm.  29.2 Spike Length cm.  20.8 Peduncle Length cm.  9.1 BranchNumber  9.8 Glume Band ABSENT 4. Ear Number Per Stalk  1.1 Position(Attitude) UPRIGHT Length cm.  18.5 Shape SEMI-CONICAL Diameter cm. 48.7 Weight gm. 192.1 Shank Length cm.  10.3 Shank Internode  14.8 HuskBract SHORT Husk Cover cm. 2.2 Husk Color Fresh GREEN Husk Color DryBUFF Cob Diameter cm.  24.6 Shelling Percent  87.5 5. Kernel Row Number 14.8 Number Per Row  41.5 Row Direction CURVED Type DENT Length (Depth)mm.  12.5 Width mm.  9.2 Thickness  3.6 Weight of 1000K gm. 339.0Endosperm Type NORMAL Endosperm Color YELLOW *These are typical values.Values may vary due to environment. Other values that are substantiallyequivalent are also within the scope of the invention. Substantiallyequivalent refers to quantitative traits that when compared do not showstatistical differences of their means.

TABLE 51 MORPHOLOGICAL TRAITS FOR THE DK626 PHENOTYPE YEAR OF DATA:1992, 1993 CHARACTERISTIC VALUE* 1. Stalk Diameter (Width) cm.  2.8Anthocyanin ABSENT Nodes With Brace Roots  1.3 Brace Root Color GREENInternode Direction ZIGZAG Internode Length cm.  17.6 2. Leaf Number 19.6 Color DARK GREEN Length cm. 100.3 Width cm.  11.4 3. Tassel Lengthcm.  47.7 Spike Length cm.  26.8 Peduncle Length cm.  13.1 AttitudeCOMPACT Branch Angle INTERMEDIATE Branch Number  5.6 Anther ColorGREEN-YELLOW Glume Color GREEN Glume Band ABSENT 4. Ear Silk ColorGREEN-YELLOW Number Per Stalk  1.0 Position (Attitude) UPRIGHT Lengthcm.  18.5 Shape SEMI-CONICAL Diameter cm.  47.5 Weight gm. 224.6 ShankLength cm.  10.7 Shank Internode  8.4 Husk Bract SHORT Husk Cover cm. 4.0 Husk Opening OPEN Husk Color Fresh GREEN Husk Color Dry BUFF CobDiameter cm.  25.5 Cob Color WHITE Cob Strength STRONG Shelling Percent 88.5 5. Kernel Row Number  19.6 Number Per Row  39.7 Row DirectionCURVED Type DENT Cap Color YELLOW Side Color DEEP YELLOW Length (Depth)mm.  13.4 Width mm.  7.4 Thickness  4.1 Weight of 1000K gm. 303.0Endosperm Type NORMAL Endosperm Color YELLOW *These are typical values.Values may vary due to environment. Other values that are substantiallyequivalent are also within the scope of the invention. Substantiallyequivalent refers to quantitative traits that when compared do not showstatistical differences of their means.XX. Genetic Complements

In another aspect, the present invention provides a genetic complementof a plant of this invention. In one embodiment, therefore, the presentinvention contemplates an inbred genetic complement of the inbred cornplants designated, separately, GMLEA, 6F905, 91CSV-1, 91DFA-5, WDAQ2,WDDQ1, 85DGD1, PHEI4, 01ASB1, 01IBH2, NL054B and FBMU. In anotherembodiment, the present invention contemplates a hybrid geneticcomplement formed by the combination of a haploid genetic complementfrom GMLEA, 6F905, 91CSV-1, 91DFA-5, WDAQ2, WDDQ1, 85DGD1, PHEI4,01ASB1, 01IBH2, NL054B or FBMU and another haploid genetic complement.Means for determining a genetic complement are well-known in the art.

As used herein, the phrase “genetic complement” means an aggregate ofnucleotide sequences, the expression of which sequences defines thephenotype of a corn plant or a cell or tissue of that plant. By way ofexample, a corn plant is genotyped to determine the array of theinherited markers it possesses. Markers are alleles at a single locus.They are preferably inherited in codominant fashion so that the presenceof both alleles at a diploid locus is readily detectable, and they arefree of environmental variation, i.e., their heritability is 1. Thisgenotyping is preferably performed on at least one generation of thedescendant plant for which the numerical value of the quantitative traitor traits of interest are also determined. The array of single locusgenotypes is expressed as a profile of marker alleles, two at eachlocus. The marker allelic composition of each locus can be eitherhomozygous or heterozygous. Homozygosity is a condition where bothalleles at a locus are characterized by the same nucleotide sequence.Heterozygosity refers to different conditions of the gene at a locus.Markers that are used for purposes of this invention include restrictionfragment length polymorphisms (RFLPs) and isozymes.

A plant genetic complement can be defined by a genetic marker profilesthat can be considered “fingerprints” of a genetic complement. Forpurposes of this invention, markers are preferably distributed evenlythroughout the genome to increase the likelihood they will be near aquantitative trait loci (QTL) of interest (e.g., in tomatoes, Nienhuiset al., 1987). These profiles are partial projections of a sample ofgenes. One of the uses of markers in general is to exclude, oralternatively include, potential parents as contributing to offspring.

Phenotypic traits characteristic of the expression of a geneticcomplement of this invention are distinguishable by electrophoreticseparation of DNA sequences cleaved by various restrictionendonucleases. Those traits (genetic markers) are termed RFLP(restriction fragment length polymorphisms).

Restriction fragment length polymorphisms (RFLPs) are geneticdifferences detectable by DNA fragment lengths, typically revealed byagarose gel electrophoresis, after restriction endonuclease digestion ofDNA. There are large numbers of restriction endonucleases available,characterized by their nucleotide cleavage sites and their source, e.g.,Eco RI. Variations in RFLPs result from nucleotide base pair differenceswhich alter the cleavage sites of the restriction endonucleases,yielding different sized fragments.

Means for performing RFLP analyses are well known in the art.Restriction fragment length polymorphism analyses reported herein wereconducted by Linkage Genetics. This service is available to the publicon a contractual basis. Probes were prepared to the fragment sequences,these probes being complementary to the sequences thereby being capableof hybridizing to them under appropriate conditions well known to thoseskilled in the art. These probes were labelled with radioactive isotopesor fluorescent dyes for ease of detection. After the fragments wereseparated by size, they were identified by the probes. Hybridizationwith a unique cloned sequence permits the identification of a specificchromosomal region (locus). Because all alleles at a locus aredetectable, RFLPs are codominant alleles, thereby satisfying a criteriafor a genetic marker. They differ from some other types of markers, e.g,from isozymes, in that they reflect the primary DNA sequence, they arenot products of transcription or translation. Furthermore, differentRFLP genetic marker profiles result from different arrays of restrictionendonucleases.

The RFLP genetic marker profile of each of the parental inbreds andexemplary resultant hybrids were determined. Because an inbred isessentially homozygous at all relevant loci, an inbred should, in almostall cases, have only one allele at each locus. In contrast, a diploidgenetic marker profile of a hybrid should be the sum of those parents,e.g., if one inbred parent had the allele A at a particular locus, andthe other inbred parent had B, the hybrid is AB by inference.

RFLP genetic marker profiles of GMLEA, 6F905, 91CSV-1, 91DFA-5, WDAQ2,WDDQ1, 85DGD1, PHEI4, 01ASB1, 01IBH2, NL054B and FBMU, are is presentedin Tables 52 through 63.

TABLE 52 RFLP PROFILE OF GMLEA *Probe/Enzyme Combination M0264H F M0306HC M0445E D M0B304E B M1120S B M1236H B M1238H F M1401E B M1406H A M1447HA M1B725E B M2239H D M2297H D M2298E B M2402H D M3212S A M3247E D M3257SA M3296H A M3446S B M3B815H C M4386H B M4396E A M4444H D M4UMC19H AM4UMC31E C M4UMC31S E M5213S A M5288S B M5295E C M5408H A M5409H CM5579S B M5UMC95H C M6223E C M6252H A M6280H F M6373E A M7263E B M7391HA M7392S B M7455H B M8107S E M8110S D M8114E E M8268H A M8438E A M8585HB M8B2369S D M8UMC48E D M9209E A M9211E A M9266S G M9B713S B M9BZE BM9WAXE A M2UMC34H B M6UMC85H C M9UMC94H A M3UM121X E *Probes used todetect RFLPs are from Linkage Genetics, 1515 West 2200 South, Suite C,Salt Lake City, Utah 84119.

TABLE 53 RFLP PROFILE OF 6F905 *Probe/Enzyme Combination M1120S C M1234HE M1238H A M1401E B M1406H B M1447H A M1B725E C M2239H G M2297H C M2298EC M2402H E M3212S B M3247E B M3257S C M3296H C M3432H H M3446S B M3457EF M4386H B M4396E A M4444H B M4451H C M4UMC19H B M4UMC31E B M4UMC31S DM5213S A M5288S A M5295E D M5408H A M5409H A M5579S B M6223E C M6280H GM6373E E M7263E C M7391H A M7392S C M7455H B M8107S C M8110S D M8114E CM8268H B M8585H F M8B2369S B M8UMC4BE A M9209E A M9211E C M9266S FM2UMC34H E M6UMC85H A M9UMC94H C M3UM121X F MOUMC130 G *Probes used todetect RFLPs are from Linkage Genetics, 1515 West 2200 South, Suite C,Salt Lake City, Utah 84119.

TABLE 54 RFLP PROFILE OF 91CSV-1 *Probe/Enzyme Combination M0264H FM0445E D MOB304E D M1120S D M1236H A M1238H E M1401E A M1406H A M1447H AM1B725E C M2239H D M2298E B M2402H D M3212S A M3257S A M3296H C M3432H DM4386H B M4396E B M4444H A M4451H B M4UMC19H A M4UMC31E C M4UMC31S EM5213S A M5288S B M5295E C M5409H C M5579S A M5UMC95H B M6223E D M6252HA M6373E D M7263E C M7391H A M7392S C M7455H B M8107S D M8110S A M8114EH M8268H C M8438E B M8585H B M8B2369S D M8UMC48E C M9209E A M9211E AM9266S G M9B713S A M9BZE B M9WAXE A *Probes used to detect RFLPs arefrom Linkage Genetics, 1515 West 2200 South, Suite C, Salt Lake City,Utah 84119.

TABLE 55 RFLP PROFILE OF 91DFA-5 *Probe/Enzyme Combination M0264H GM0306H A M0445E B M1234H E M1238H F M1406H B M1447H B M1B725E B M2239H CM2297H A M2402H E M3212S B M3247E D M3257S B M3296H A M3446S F M3B815H BM4386H D M4396E A M4444H B M4451H B M4UMC19H B M4UMC31E C M4UMC31S AM5213S A M5295E D M5408H A M5409H C M6223E C M6280H B M6373E A M7263E AM7391H A M7392S C M7455H C M8110S A M8268H B M8585H A M8B2369S BM8UMC48E C M9209E A M9266S C M9BZE A M9WAXE G *Probes used to detectRFLPs are from Linkage Genetics, 1515 West 2200 South, Suite C, SaltLake City, Utah 84119.

TABLE 56 RFLP PROFILE OF WDAQ2 *Probe/Enzyme Combination M0306H A M1120SB M1234H B M1236H AC M1238H F M1406H B M1447H B M1B725E F M2239H DM2297H A M2298E C M2402H E M3212S B M3247E D M3257S B M3296H A M3432H FM3446S F M3B815H D M4386H D M4396E A M4444H A M4451H B M4UMC19H BM4UMC31E C M4UMC31S A M5213S A M5288S A M5295E D M5408H A M5409H AM5579S C M5UMC95H A M6223E C M6252H A M6280H A M7263E A M7391H A M7392SC M7455H B M8110S C M8114E B M8268H B M8585H D M8B2369S B M8UMC48E AM9209E A M9266S A M9B713S A M9BZE B M9WAXE G M2UMC34H E M6UMC85H AM9UMC94H B M3UM121X C MOUMC130 A *probes used to detect RFLPs are fromLinkage Genetics, 1515 West 2200 South, Suite C, Salt Lake City, Utah84119.

TABLE 57 RFLP PROFILE OF WDDQ1 *Probe/Enzyme Combination M0264H G M0306HA M0445E BC M0B304E D M1234H D M1236H A M1238H F M1401E A M1406H BM1447H B M1B725E B M2239H G M2297H A M2298E B M2402H E M3212S B M3247E BM3257S C M3296H F M3446S F M3B815H B M4386H B M4396E H M4444H B M4451H BM4UMC19H B M4UMC31E B M4UMC31S D M5213S A M5295E D M5408H A M5409H AM5579S C M5UMC95H A M6223E C M6252H E M6280H B M6373E E M7263E A M7391HC M7392S C M7455H A M8110S C M8114E B M8268H J M8438E A M8585H AM8B2369S D M8UMC48E A M9209E C M9211E C M9266S A M9B713S A M9BZE BM9WAXE A M2UMC34H E M6UMC85H A M9UNC94H B M3UMC121X C MOUMC130 H *Probesused to detect RFLPs are from Linkage Genetics, 1515 West 2200 South,Suite C, Salt Lake City, Utah 84119.

TABLE 58 RFLP PROFILE OF 85DGD1. *Probe/Enzyme Combination M0445E BCM0B304E A M1120S B M1234H D M1236H A M1238H F M1401E A M1406H A M1447H BM1B725E B M2239H G M2297H A M2298E B M3212S B M3257S C M3296H A M3432HGH M3446S F M3457E F M3B815H B M4396E A M4UMC19H B M5213S A M5295E DM5408H A M5409H C M6223E C M6252H E M6280H B M6373E E M7263E C M7392S CM8110S C M8114E B M8268H B M8438E A M8585H A M8UMC48E A M9209E A M9211EC M9266S A M9B713S A M9BZE B M9WAXE B M2UMC34H E M6UMC85H A M9UMC94H BM3UMC121X C MOUMC130 H *Probes used to detect RFLPs are from LinkageGenetics, 1515 West 2200 South, Suite C, Salt Lake City, Utah 84119.

TABLE 59 RFLP PROFILE OF PHEI4 *Probe/Enzyme Combination M0264H E M0306HA M0445E D MOB304E C M1120S E M1234H A M1236H C M1238H E M1401E A M1406HB M1447H A M1B725E B M2239H A M2297H E M2298E C M3212S C M3247E D M3257SB M3296H E M3432H DF M3446S C M3457E E M3B815H B M4386H I M4396E HM4451H B M4UMC19H A M4UMC31E CD M5213S B M5288S AG M5295E C M5408H AM5409H C M5579S B M5UMC95H B M6223E B M6252H A M6280H G M6373E A M7263EB M7391H C M7392S B M7455H A M8107S D M8110S A M8114E B M8268H K M8438EB M8585H A M8B2369S D M8UMC48E C M9209E A M9211E C M9266S A M9B713S AM9BZE A M9WAXE B M2UMC34H E M6UMC85H A M9UMC94H F M3UMC121X C MOUMC130 C*Probes used to detect RFLPs are from Linkage Genetics, 1515 West 2200South, Suite C, Salt Lake City, Utah 84119.

TABLE 60 RFLP PROFILE OF 01ASB1 *Probe/Enzyme Combination M0306H EM0445E B M1234H D M1238H A M1401E A M1406H A M1447H B M2239H C M2297K AM2402H E M3212S B M3247E B M3257S C M3296H C M3446S B M3457E E M4386H AM4396E A M4444H B M4UMC19U A M4UMC31E C M4UMC31S A M5213S A M5288S AM5295E D M5408K A M5409H C M5579S A M5UMC95H A M6223E B M6252H E M6280HB M6373E G M7263E C M7391H C M7392S C M8110S C M8114E D M8268H B M8585HA M8B2369S B M8UMC48E A M9209E C M9211E G M9266S H M9B713S A M2UMC34H DM6UMC85H A M9UMC94H B M3UMC121X C MOUMC130 H *Probes used to detectRFLPs are from Linkage Genetics, 1515 West 2200 South, Suite C, SaltLake City, Utah 84119.

TABLE 61 RFLP PROFILE OF 01IBH2 *Probe/Enzyme Combination M0264H HM0306H A M1120S C M1234H I M1236H AC M1238H E M1406H B M1447H A M1B725 FM2239H D M2297H B M2298E C M2402H C M3212S A M3247E B M3257S A M3296H EM3446S C M3457E E M4386H D M4396E H M4444H A M4451H B M4UMC19H AM4UMC31E B M4UMC31S D M5213S A M5288S B M5295E A M5408H A M5409H CM5579S A M5UMC95H B M6223E C M6252H A M6280H G M6373E E M7263E A M7391HA M7392S B M7455H B M8107S D M8110S A M8114E F M8268H K M8585H BM8B2369S D M9209E A M9211E C M9266S B M9B713S A M2UMC34H D M6UMC85H CM9UMC94H E M3UMC121X A MOUMC130 C *Probes used to detect RFLPs are fromLinkage Genetics, 1515 West 2200 South, Suite C, Salt Lake City, Utah84119.

TABLE 62 RFLP PROFILE OF NL054B *Probe/Enzyme Combination M0264H GM0306H A M0445E B M1234H D M1236H AC M1238H F M1401E C M1406H A M1447H BM1B725E B M2239H C M2297H A M2298E C M2402H E M3212S B M3247E D M3257S CM3296H A M3432H H M3B815H B M4386H D M4396E H M4444H B M4UMC19H BM4UMC31E C M4UMC31S A M5213S A M5295E D M5408H A M5409H A M5UMC95H AM6223E C M6252H E M6280H B M6373E J M7263E A M7391H A M7392S C M7455H AM8110S C M8114E B M8268H B M8585H A M8B2369S B M8UMC48E A M9209E AM9211E G M9B713S AB M9BZE A M9WAXE B M2UMC34H E M6UMC85H A M9UMC94H BM3UHC121X C MOUMC130 H *Probes used to detect RFLPs are from LinkageGenetics, 1515 West 2200 South, Suite C, Salt Lake City, Utah 84119.

TABLE 63 RFLP PROFILE OF FBMU *Probe/Enzyme Combination M0264H G M0306HB M0445E B M0B304E A M1120S F M1234H D M1236H A M1238H F M1401E C M1406HA M1447H B M1B725 C M2239H C M2297H A M2298E B M2402H E M3212S B M3247EB M3257S B M3432H I M3446S B M3457E E M3B815H B M4386H B M4396E H M4444HB M4451H B M4UMC19H A M4UMC31E B M4UMC31S D M5213S C M5288S A M5295E DM5408H A M5409H A M5579S B M5UMC95H A M6223E B M6252H D M6280H E M6373EG M7263E A M7391H C M7392S C M7455H A M8110S B M8114E D M8268H B M8438EA M8585H A M8B2369S D M8UMC48E C M9209E C M9211E G M9266S H M9B713S AM9BZE A M9WAXE A *Probes used to detect RFLPs are from Linkaqe Genetics,1515 West 2200 South, Suite C, Salt Lake City, Utah 84119.

Another aspect of this invention is a plant genetic complementcharacterized by a genetic isozyme typing profile. Isozymes are forms ofproteins that are distinguishable, for example, on starch gelelectrophoresis, usually by charge and/or molecular weight. Thetechniques and nomenclature for isozyme analysis are described in,Stuber et al. (1988), which is incorporated by reference.

A standard set of loci can be used as a reference set. Comparativeanalysis of these loci is used to compare the purity of hybrid seeds, toassess the increased variability in hybrids compared to inbreds, and todetermine the identity of seeds, plants, and plant parts. In thisrespect, an isozyme reference set can be used to develop genotypic“fingerprints.”

Tables 64 through 75 list the identifying numbers of the alleles atisozyme loci types. Tables 64 through 75 represent the exemplary geneticisozyme typing profiles for GMLEA, 6F905, 91CSV-1, 91DFA-5, WDAQ2,WDDQ1, 85DGD1, PHEI4, 01ASB1, 01IBH2, NL054B and FBMU, respectively.

TABLE 64 ISOZYME PROFILE OF GMLEA ISOZYME LOCUS ALLELE Acph-1 4 Adh-1 4Cat-3 9 Got-1 4 Got-2 4 Got-3 4 Idh-1 4 Idh-2 6 Mdh-1  6* Mdh-2 6 Mdh-316  Mdh-4 12  Mdh-5 12  6-Pgd-1 2 6-Pgd-2 5 Pgm-1 9 Pgm-2 4 Phi-1 4 # ofseeds analyzed: 12 *Allele is probably a 6, but null cannot be ruledout.

TABLE 65 ISOZYME PROFILE OF 6F905 LOCUS ISOZYME ALLELE Acph-1 2 Adh-1 4Cat-3 9 Got-1 4 Got-2 4 Got-3 4 Idh-1 4 Idh-2 4 Mdh-1 6 Mdh-2 3.5 Mdh-316 Mdh-4 12 Mdh-5 12 6-Pgd-1 3.8 6-Pgd-2 5 Pgm-1 9 Pgm-2 4 Phi-1 4 # ofseeds analyzed: 6

TABLE 66 ISOZYME PROFILE OF 91CSV-1 LOCUS ISOZYME ALLELE Acph-1 3 Adh-14 Cat-3 9 Got-1 4 Got-2 4 Got-3 4 Idh-1 4 Idh-2 4 Mdh-1  6* Mdh-2 6Mdh-3 16  Mdh-4 12  Mdh-5 12  6-Pgd-1   3.8 6-Pgd-2 5 Pgm-1 9 Pgm-2 4Phi-1 4 # of seeds analyzed: 18 *Allele is probably a 6, but null cannotbe ruled out.

TABLE 67 ISOZYME PROFILE OF 91DFA-5 LOCUS ISOZYME ALLELE Acph-1 4 Adh-14 Cat-3 9 Got-1 4 Got-2 4 Got-3 4 Idh-1 4 Idh-2 4 Mdh-1 — Mdh-2 3.5Mdh-3 16 Mdh-4 12 Mdh-5 12 6-Pgd-1 3.8 6-Pgd-2 5 Pgm-1 9 Pgm-2 4 Phi-1 4# of seeds analyzed: 6

TABLE 68 ISOZYME PROFILE OF WDAQ2 LOCUS ISOZYME ALLELE Acph-1 4 Adh-1 4Cat-3 9 Got-1 4 Got-2 2 Got-3 4 Idh-1 4 Idh-2 6 Mdh-1  6* Mdh-2 6 Mdh-316  Mdh-4 12  Mdh-5 12  6-Pgd-1 NS** 6-Pgd-2 NS** Pgm-1 9 Pgm-2 4 Phi-14 # of seeds analyzed: 18 *Allele is probably a 6, but null cannot beruled out. **NS - enzyme system was not scorable.

TABLE 69 ISOZYME PROFILE OF WDDQ1 LOCUS ISOZYME ALLELE Acph-1 2 Adh-1 4Cat-3 9 Got-1 4 Got-2 2 Got-3 4 Idh-1 4 Idh-2 4 Mdh-1 6 Mdh-2 3.5 Mdh-316 Mdh-4 12 Mdh-5 12 6-Pgd-1 3.8 6-Pgd-2 5 Pgm-1 9 Pgm-2 4 Phi-1 4 # ofseeds analyzed: 6

TABLE 70 ISOZYME PROFILE OF 85DGD1 LOCUS ISOZYME ALLELE Acph-1 Adh-1 4Cat-3 9 Got-1 4 Got-2 4 Got-3 4 Idh-1 4 Idh-2 4 Mdh-1 6 Mdh-2 3.5 Mdh-316 Mdh-4 12 Mdh-5 12 6-Pgd-1 3.8 6-Pgd-2 5 Pgm-1 9 Pgm-2 4 Phi-1 4 # ofseeds analyzed: 6

TABLE 71 ISOZYME PROFILE OF PHEI4 LOCUS ISOZYME ALLELE Acph-1 2 Adh-1 4Cat-3 9 Got-1 4 Got-2 4 Got-3 4 Idh-1 4 Idh-2 6 Mdh-1 6 Mdh-2 3.5 Mdh-316 Mdh-4 12 Mdh-5 12 6-Pgd-1 3.8 6-Pgd-2 5 Pgm-1 9 Pgm-2 4 Phi-1 4 # ofseeds analyzed: 6

TABLE 72 ISOZYME PROFILE OF 01ASB1 LOCUS ISOZYME ALLELE Acph-1 2 Adh-1 4Cat-3 9 Got-1 4 Got-2 4 Got-3 4 Idh-1 4 Idh-2 4 Mdh-1 6 Mdh-2 3.5 Mdh-316 Mdh-4 12 Mdh-5 12 6-Pgd-1 3.8 6-Pgd-2 5 Pgm-1 9 Pgm-2 4 Phi-1 5 # ofseeds analyzed: 6

TABLE 73 ISOZYME PROFILE OF 01IBH2 LOCUS ISOZYME ALLELE Acph-1 2 Adh-1 4Cat-3 9 Got-1 4 Got-2 4 Got-3 4 Idh-1 4 Idh-2 NS* Mdh-1 6 Mdh-2 NS*Mdh-3 16 Mdh-4 12 Mdh-5 12 6-Pgd-1 3.8 6-Pgd-2 5 Pgm-1 9 Pgm-2 4 Phi-1 4# of seeds analyzed: 12 *NS - enzyme system was not scorable.

TABLE 74 ISOZYME PROFILE OF NL054B LOCUS ISOZYME ALLELE Acph-1 2 Adh-1 4Cat-3 9 Got-1 4 Got-2 4 Got-3 NS* Idh-1 4 Idh-2 4 Mdh-1 6 Mdh-2 3.5Mdh-3 16 Mdh-4 12 Mdh-5 12 6-Pgd-1 2 6-Pgd-2 5 Pgm-1 9 Pgm-2 4 Phi-1 4 #of seeds analyzed: 6 *NS - enzyme system was not scorable.

TABLE 75 IBOZYME PROFILE OF FBMU LOCUS ISOZYME ALLELE Acph-1 2 Adh-1 4Cat-3 9 Got-1 4 Got-2 4 Got-3 4 Idh-1 4 Idh-2 4 Mdh-1 6 Mdh-2 3.5 Mdh-316 Mdh-4 12 Mdh-5 12 6-Pgd-1 3.8 6-Pgd-2 5 Pgm-1 9 Pgm-2 4 Phi-1 4 # ofseeds analyzed: 6

The present invention also contemplates a hybrid genetic complementformed by the combination of a haploid genetic complement of the cornplant GMLEA, 6F905, 91CSV-1, 91DFA-5, WDAQ2, WDDQ1, 85DGD1, PHEI4,01ASB1, 01IBH2, NL054B or FBMU, with a haploid genetic complement of asecond corn plant. Means for combining a haploid genetic complement fromany of the foregoing inbreds with another haploid genetic complement canbe any method hereinbefore for producing a hybrid plant from GMLEA,6F905, 91CSV-1, 91DFA-5, WDAQ2, WDDQ1, 85DGD1, PHEI4, 01ASB1, 01IBH2,NL054B or FBMU. It is also contemplated that a hybrid genetic complementcan be prepared using in vitro regeneration of a tissue culture of ahybrid plant of this invention.

A hybrid genetic complement contained in the seed of a hybrid derivedfrom GMLEA, 6F905, 91CSV-1, 91DFA-5, WDAQ2, WDDQ1, 85DGD1, PHEI4,01ASB1, 01IBH2, NL054B or FBMU is a further aspect of this invention.Exemplary hybrid genetic complements are the genetic complements of thehybrids DK706, DK446, DK474, DK642, DK604, DK560, DK566, DK442, DK527and DK626.

Tables 76 through 85 show the identifying numbers of the alleles for thehybrids DK706, DK446, DK604, DK527, DK442, DK474, DK642, DK560, DK566,and DK626, which are exemplary RFLP genetic marker profiles for hybridsderived from the inbreds of the present invention.

Table 76 concerns DK706, which has GMLEA and 6F905, both inbreds of theinvention, as parents. Table 77 concerns DK446, which has 91CSV-1 and01ASB1, both inbreds of the invention, as parents. Table 78 concernsDK604, which has WDAQ2 and 01IBH2, both inbreds of the invention, asparents. Table 79 concerns DK527, which has 01IBH2 and FBMU, bothinbreds of the invention, as parents. Table 80 concerns DK442, whichalso has 01IBH2 as one inbred parent.

Table 81 concerns DK474, which has 91DFA-5 as one inbred parent. Table82 concerns DK642, which has WDDQ1 as a parent. Table 83 concerns DK560,which has 85DGD1 as one inbred parent. Table 84 concerns DK566, whichhas PHEI4 as a parent. Table 85 concerns DK626, which has NL054B as oneinbred parent.

TABLE 76 RFLP PROFILE FOR DK706 *Probe/Enzyme Combination Allelic PairM1120S BC M1238H AF M1401E BB M1406H AB M1447H AA M1B725E BC M2239H DGM2297H CD M2298E BC M2402H DE M3212S AB M3247E BD M3257S AC M3296H ACM3446S BB M4386H BB M4396E AA M4444H BD M4UMC19H AB M4UMC31E BC M4UMC31SDE M5213S AA M5288S AB M5295E CD M5408H AA M5409H AC M5579S BB M6223E CCM6280H FG M6373E AE M7263E BC M7391H AA M7392S BC M7455H BB M8107S CEM8110S DD M8114E CE M8268H AD M8585H BF M8B2369S BD M8UMC48E AD M9209EAA M9211E AC M9266S FG M2UMC34H BE M6UMC85H AC M9UMC94H AC M3UM121X EF*Probes used to detect RFLPs are from Linkage Genetics, 1515 West 2200South, Su1te C, Salt Lake C1ty, Utah 84119.

TABLE 77 RFLP PROFILE FOR DK446 *Probe/Enzyme Combination Allelic PairM0445E BD M1238H AE M1401E AA M1406H AA M1447H AB M2239H CD M2402H DEM3212S AB M3257S AC M3296H CC M4386H AB M4396E AB M4444H AB M4UMC19H AAM4UMC31E CC M4UMC31S AE M5213S AA M5288S AB M5295E CD M5409H CC M5579SAA M5UMC95H AB M6223E BD M6252H AE M6373E DG M7263E CC M7391H AC M7392SCC M8110S AC M8114E DH M8268H BC M8585H AB M8B2369S BD M8UMC48E ACM9209E AC M9211E AG M9266S GH M9B713S AA *Probes used to detect RFLPsare from Linkage Genetics, 1515 West 2200 South, Suite C, Salt LakeCity, Utah 84119.

TABLE 78 RFLP PROFILE FOR DK604 *Probe/Enzyme Combination Allelic PairM0306H AA M11205 BC M1234H BI M1236H AC M1238H EF M1406H BB M1447H ABM1B725E FF M2239H DD M2297H AB M2298E CC M2402H CE M3212S AB M3247E BDM3257S AB M3296H AE M3446S CF M3B815H DD M4396E AH M4444H AA M4451H BBM4UMC19H AB M4UMC31E BC M4UMC31S AD M5213S AA M5295E AD M5408H AA M5409HAC M5579S AC M5UMC95H AB M6223E CC M6252H AA M6280H AG M7263E AA M7391HAA M7392S BC M7455H BB M8110S AC M8114E BF M8268H BK M8585H BD M8B2369SBD M9209E AA M9266S AB M9B713S AA M9BZE BB M9WAXE BG M2UMC34H DEM6UMC85H AC M9UMC94H BE M3UM121X AC MOUMC130 AC *Probes used to detectRFLPs are from Linkage Genetics, 1515 West 2200 South, Suite C, SaltLake City, Utah 84119.

TABLE 79 RFLP PROFILE FOR DK527 *Probe/Enzyme Combination Allelic PairM0264H GH M0306H AD M0445E ABC M1120S CF M1234H DI M1236H AAC M1238H EFM1406H AB M1447H AB M1B725E CF M2239H CD M2297H AB M2298E BC M2402H CEM3212S AB M3247E EB M3257S AB M3446S BC M3457E EE M3B815H BD M4396E HHM4444H AB M4451H BB M4UMC19H AA M4UMC31E BB M4UMC31S DD M5213S AC M5295EAD M5408H AA M5409H AC M5579S AB M5UMC95H AB M6223E BC M6252H AD M6280HEG M6373E EG M7263E AA M7391H AC M7392S BC M7455H AB M8110S AD M8114E DFM8268H BK M8438E AB M8585H AB M8B2369S DD M9209E AC M9211E CG M9266S BHM9B713S AA M9BZE AB M9WAXE AB *Probes used to detect RFLPs are fromLinkage Genetics, 1515 West 2200 South, Suite C, Salt Lake City, Utah84119.

TABLE 80 RFLP PROFILE FOR DK442 *Probe/Enzyme Allelic Pair CombinationDK442 M0264H HL M0306H AE M0445E ABC M1120S CD M1234H EI M1236H AACM1238H AE M1406H BB M1447H AE M1B725E — M2239H — M2297H BC M2298E —M2402H CE M3212S AA M3247E BB M3257S — M3296H DE M3446S CF M3457E EEM3B815H DD M4396E FH M4444H AA M4451H BB M4UMC19H AA M4UMC31E — M4UMC31SBD M5213S AB M5295E AD M5408H AA M5409H AC M5579S AC M5UMC95H — M6223E —M6252H AE M6280H AG M6373E AE M7263E AA M7391H AA M7392S BB M7455H BCM8110S AD M8114E EF M8268H BK M8438E AB M8585H BB M8B2369S DD M9209E AAM9211E — M9266S — M9B713S AB M9BZE — M9WAXE AB M2UMC34H — M6UMC85H —M9UMC94H — M3UM121X — MOUMC130 — *Probes used to detect RFLPs are fromLinkage Genetics, 1515 West 2200 South, Suite C, Salt Lake City, Utah84119.

TABLE 81 RFLP PROFILE FOR DK474 *Probe/Enzyme Combination Allelic PairM0264H FG M0306H AC M0445E BD M1234H AE M1238H FF M1406H AB M1447H BBM1B725E BH M2297H AD M2402H DE M3296H AC M3B815H BC M4386H BD M4396E ABM4444H AB M4451H BB M4UMC19H AB M4UMC31E CC M5213S AA M5295E CD M5408HAA M5409H CC M6223E CC M6280H BF M6373E AD M7263E AC M7391H AA M7455H BCM8110S AA M8268H BC M9209E AA M9BZE AB M9WAXE AG *Probes used to detectRFLPs are from Linkage Genetics, 1515 West 2200 South, Suite C, SaltLake City, Utah 84119.

TABLE 82 RFLP PROFILE FOR DK642 *Probe/Enzyme Combination Allelic PairM0264H FG M0306H AC M1234H AD M1238H FF M1401E AA M1406H AB M1447H ABM1B725E BB M2297H AD M2298E BB M2402H DE M3296H AF M3B815H BD M4386H BBM4396E BH M4444H AB M4451H AB M4UMC19H BC M4UMC31E BC M5213S AB M5295ECD M5409H AC M5UMC95H AC M6223E CC M6252H AB M6280H BI M6373E AE M7391HAC M7455H AB M8110S AC M8114E BD M8268H BJ M8438E AD M8UMC48E AC M9209EAC M9211E AC M9B713S AB M9BZE BB M9WAXE AA *Probes used to detect RFLPsare from Linkage Genetics, 1515 West 2200 South, Suite C, Salt LakeCity, Utah 84119.

TABLE 83 RFLP PROFILE FOR DK560 *Probe/Enzyme Combination Allelic PairM0445E BCD M0B304E AC M1234H ADE M1236H AA M1238H EF M1401E AA M1406H ABM1447H BB M2239H AG M2297H AC M2298E BC M32125 BC M3257S BC M3432H FGHM3B815H BB M4396E AH M52135 AB M5295E CD M5408H AA M5409H CC M6223E CCM6252H AE M6373E AE M7263E BC M7392S BC M8110S CC M8114E BE M8438E ABM8585H AD M8UMC48E AC M9209E AA M9211E CC M9266S AC M9B713S AB M9BZE ABM9WAXE BG M2UMC34H EE M6UMC85H AA M9UMC94H BB M3UMC121X CC MOUMC130 AH*Probes used to detect RFLPs are from Linkage Genetics, 1515 West 2200South, Suite C, Salt Lake City, Utah 84119.

TABLE 84 RFLP PROFILE FOR DK566 *Probe/Enzyme Combination Allelic PairM0264H EG M0306H AA M0445E BD M1120S BE M1234H AK M1238H AE M1401E ACM1406H AB M1447H AD M1B725E BC M2297H AE M2298E BC M3296H AE M3432H DFIM3457E EE M3B815H BB M4386H BI M4396E HH M4451H BB M4UMC19H AA M5213S ABM5295E CD M5408H AA M5409H CC M5UMC95H AB M6223E BC M6252H AE M6280H DGM6373E AE M7263E BC M7391H CC M7455H AA M8107S DF M8110S AC M8114E BBM8268H BK M8438E AB M8UMC48E CC M9209E AC M9211E CC M9B713S AA M9BZE ABM9WAXE AB *Probes used to detect RFLPs are from Linkage Genetics, 1515West 2200 South, Suite C, Salt Lake City, Utah 84119.

TABLE 85 RFLP PROFILE FOR DK626 *Probe/Enzyme Combination Allelic PairM0264H FG M0306H AC M0445E BD M1234H AD M1238H FF M1401E AC M1406H AAM1447H AB M1B725E BB M2297H AD M2298E BC M2402H DE M3296H AA M3B815H BDM4386H BD M4396E BH M4444H AB M4UMC19H BC M4UMC31E CC M5213S AB M5295ECD M5409H AC M5UMC95H AC M6223E CC M6252H AE M6280H BI M6373E AJ M7391HAA M7455H AB M8110S AC M8114E BD M8268H BB M8UMC48E AC M9209E AA M9211EAG M9BZE AB M9WAXE AB *Probes used to detect RFLPs are from LinkageGenetics, 1515 West 2200 South, Suite C, Salt Lake City, Utah 84119.

The exemplary hybrid genetic complements of hybrids DK706, DK446, DK604,DK527, DK442, DK474, DK642, DK560, DK566, and DK626, may also beassessed by genetic isozyme typing profiles using a standard set of locias a reference set, using, e.g., the same, or a different, set of locito those described above. Tables 86 through 95 list the identifyingnumbers of the alleles at isozyme loci types and present the exemplarygenetic isozyme typing profiles for the hybrids DK706, DK446, DK604,DK527, DK442, DK474, DK642, DK560, DK566, and DK626, which are exemplaryhybrids derived from the inbreds of the present invention.

Table 86 concerns DK706, which has GMLEA and 6F905, both inbreds of theinvention, as parents. Table 87 concerns DK446, which has 91CSV-1 and01ASB1, both inbreds of the invention, as parents. Table 88 concernsDK604, which has WDAQ2 and 01IBH2, both inbreds of the invention, asparents. Table 89 concerns DK527, which has 01IBH2 and FBMU, bothinbreds of the invention, as parents. Table 90 concerns DK442, whichalso has 01IBH2 as one inbred parent.

Table 91 concerns DK474, which has 91DFA-5 as one inbred parent. Table92 concerns DK642, which has WDDQ1 as a parent. Table 93 concerns DK560,which has 85DGD1 as one inbred parent. Table 94 concerns DK566, whichhas PHEI4 as a parent. Table 95 concerns DK626, which has NL054B as oneinbred parent.

TABLE 86 ISOZYME GENOTYPE FOR HYBRID DK706 ISOZYME ALLELE LOCUS Acph-12/4 Adh-1 4 Cat-3 9 Got-1 4 Got-2 4 Got-3 4 Idh-1 4 Idh-2 4/6 Mdh-1 6Mdh-2 3.5/6   Mdh-3 16  Mdh-4 12  Mdh-5 12  6-Pgd-1   2/3.8 6-Pgd-2 5Pgm-1 9 Pgm-2 4 Phi-1 4

TABLE 87 ISOZYME GENOTYPE FOR HYBRID DK446 ISOZYME ALLELE LOCUS Acph-12/3 Adh-1 4 Cat-3 9 Got-1 4 Got-2 4 Got-3 4 Idh-1 4 Idh-2 4 Mdh-1 6Mdh-2 3.5/6   Mdh-3 16 Mdh-4 12 Mdh-5 12 6-Pgd-1   3.8 6-Pgd-2 5 Pgm-1 9Pgm-2 4 Phi-1 4/5

TABLE 88 ISOZYME GENOTYPE FOR HYBRID DK604 LOCUS ISOZYME ALLELE Acph-12/4 Adh-1 4 Cat-3 9 Got-1 4 Got-2 2/4 Got-3 4 Idh-1 4 Idh-2 NS* Mdh-1 6Mdh-2 NS* Mdh-3 16  Mdh-4 12  Mdh-5 12  6-Pgd-1 NS* 6-Pgd-2 NS* Pgm-1 9Pgm-2 4 Phi-1 4 *NS - enzyme system was not scorable.

TABLE 89 ISOZYME GENOTYPE FOR HYBRID DK527 LOCUS ISOZYME ALLELE Acph-1 2Adh-1 4 Cat-3 9 Got-1 4 Got-2 4 Got-3 4 Idh-1 4 Idh-2 NS* Mdh-1 6 Mdh-2NS* Mdh-3 16  Mdh-4 12  Mdh-5 12  6-Pgd-1   3.8 6-Pgd-2 5 Pgm-1 9 Pgm-24 Phi-1 4 *Enzyme system was not scorable.

TABLE 90 ISOZYME GENOTYPE FOR HYBRID DK442 ISOZYME ALLELES LOCUS DK442Acph-1 2 Adh-1 4 Cat-3 9 Got-1 4 Got-2 4 Got-3 4 Idh-1 4 Idh-2 4/6 Mdh-16 Mdh-2 3/6 Mdh-3 16  Mdh-4 12  Mdh-5 12  6-Pgd-1   3.8 6-Pgd-2 5 Pgm-19 Pgm-2 4 Phi-1 4 * Enzyme system was not scorable.

TABLE 91 ISOZYME GENOTYPE FOR HYBRID DK474 LOCUS ISOZYME ALLELE Acph-13/4 Adh-1 4 Cat-3 9 Got-1 4 Got-2 4 Got-3 4 Idh-1 4 Idh-2 4 Mdh-1 —Mdh-2 3.5/6   Mdh-3 16  Mdh-4 12  Mdh-5 12  6-Pgd-1   3.8 6-Pgd-2 5Pgm-1 9 Pgm-2 4 Phi-1 4

TABLE 92 ISOZYME GENOTYPE FOR HYBRID DK642 LOCUS ISOZYME ALLELE Acph-12/4 Adh-1 4 Cat-3 9 Got-1 4 Got-2 2/4 Got-3 4 Idh-1 4 Idh-2 4/6 Idh-1 6Mdh-2 3.5/6   Mdh-3 16  Mdh-4 12  Mdh-5 12  6-Pgd-1   2/3.8 6-Pgd-2 5Pgm-1 9 Pgm-2 4 Phi-1 4

TABLE 93 ISOZYME GENOTYPE FOR HYBRID DK560 LOCUS ISOZYME ALLELE Acph-12/2; 2/4 Adh-1 4 Cat-3 9 Got-1 4 Got-2 4 Got-3 NS* Idh-1 4 Idh-2 4/6Mdh-1 6 Mdh-2   3.5 Mdh-3 16  Mdh-4 12  Mdh-5 12  6-Pgd-1   3.8 6-Pgd-25 Pgm-1 9 Pgm-2 4 Phi-1 4 *NS - Enzyme system was not scorable.

TABLE 94 ISOZYME GENOTYPE FOR HYBRID DK566 LOCUS ISOZYME ALLELE Acph-1 2Adh-1 4 Cat-3 9 Got-1 4 Got-2 4 Got-3 4 Idh-1 4 Idh-2 6 Mdh-1 6 Mdh-23.5 Mdh-3 16 Mdh-4 12 Mdh-5 12 6-Pgd-1 3.8 6-Pgd-2 5 Pgm-1 9 Pgm-2 4Phi-1 4

TABLE 95 ISOZYME GENOTYPE FOR HYBRID DK626 LOCUS ISOZYME ALLELE Acph-12/4 Adh-1 4 Cat-3 9 Got-1 4 Got-2 4 Got-3 NS* Idh-1 4 Idh-2 4/6 Mdh-1 6Mdh-2 3.5/6 Mdh-3 16 Mdh-4 12 Mdh-5 12 6-Pgd-1 2 6-Pgd-2 5 Pgm-1 9 Pgm-24 Phi-1 4 *NS - enzyme system was not scorable.

All of the compositions and methods disclosed and claimed herein can bemade and executed without undue experimentation in light of the presentdisclosure. While the compositions and methods of this invention havebeen described in terms of the foregoing illustrative embodiments, itwill be apparent to those of skill in the art that variations, changes,modifications and alterations may be applied to the composition,methods, and in the steps or in the sequence of steps of the methodsdescribed herein, without departing from the true concept, spirit andscope of the invention. More specifically, it will be apparent thatcertain agents that are both chemically and physiologically related maybe substituted for the agents described herein while the same or similarresults would be achieved. All such similar substitutes andmodifications apparent to those skilled in the art are deemed to bewithin the spirit, scope and concept of the invention as defined by theappended claims.

REFERENCES

The following references, to the extent that they provide exemplaryprocedural or other details supplementary to those set forth herein, arespecifically incorporated herein by reference.

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1. Inbred corn seed of the corn plant designated 85DGD1, a sample ofsaid seed having been deposited under ATCC Accession No. PTA-6160.
 2. Anessentially homogeneous population of corn seed consisting essentiallyof inbred corn seed designated 85DGD1, a sample of the seeds of saidcorn plant 85DGD1 having been deposited under ATCC Accession No.PTA-6160.
 3. An essentially homogeneous population of corn seedconsisting essentially of the inbred corn seed of claim 1, andessentially free from hybrid seed.
 4. A corn plant produced by growingthe seed of claim
 1. 5. Pollen of the plant of claim
 4. 6. An ovule ofthe plant of claim
 4. 7. An essentially homogeneous population of cornplants consisting essentially of corn plants produced by growing theseed of claim
 1. 8. A corn plant having all the physiological andmorphological characteristics of the inbred corn plant 85DGD1, a sampleof the seed of said corn plant 85DGD1 having been deposited under ATCCAccession No. PTA-6160.
 9. A tissue culture of regenerable cells ofinbred corn plant 85DGD1, wherein the tissue regenerates plants havingall the physiological and morphological characteristics of corn plant85DGD1, a sample of the seed of said corn plant 85DGD1 having beendeposited under ATCC Accession No. PTA-6160.
 10. The tissue culture ofclaim 9, wherein the regenerable cells are from embryos, meristematiccells, pollen, leaves, anthers, roots, root tips, silk, flowers,kernels, ears, cobs, husks, stalks, or protoplasts or callus derivedtherefrom.
 11. A corn plant regenerated from the tissue culture of claim9, having all the physiological and morphological characteristics ofcorn plant 85DGD1.
 12. An inbred corn plant cell of the corn plant ofclaim 4 having: (a) the RFLP genetic marker profile shown in Table 58;or (b) the genetic isozyme typing profile shown in Table
 70. 13. Theinbred corn plant cell of claim 12, having the RFLP genetic markerprofile shown in Table
 58. 14. The inbred corn plant cell of claim 12,having the genetic isozyme typing profile shown in Table
 70. 15. Theinbred corn plant cell of claim 12, having the RFLP genetic markerprofile and genetic isozyme typing profile shown in Tables 58 and 70.16. A process of preparing corn seed, comprising crossing a first parentcorn plant with a second parent corn plant, wherein said first or secondcorn plant is the inbred corn plant 85DGD1, a sample of the seed of saidinbred corn plant 85DGD1 having been deposited under ATCC Accession No.PTA-6160.
 17. The process of claim 16, further defined as a process ofpreparing hybrid corn seed, comprising crossing a first inbred cornplant with a second, distinct inbred corn plant, wherein said first orsecond inbred corn plant is the inbred corn plant 85DGD1, a sample ofthe seed of said inbred corn plant 85DGD1 having been deposited underATCC Accession No. PTA-6160.
 18. The process of claim 17, whereincrossing comprises the steps of: (a) planting in pollinating proximityseeds of said first and second inbred corn plants; (b) cultivating theseeds of said first and second inbred corn plants into plants that bearflowers; (c) emasculating the male flowers of said first or secondinbred corn plant to produce an emasculated corn plant; (d) allowingcross-pollination to occur between said first and second inbred cornplants; and (e) harvesting seeds produced on said emasculated cornplant.
 19. The process of claim 18, further comprising growing saidharvested seed to produce a hybrid corn plant.
 20. A method of producinga male sterile corn plant comprising transforming the corn plant ofclaim 4 with a nucleic acid molecule that confers male sterility.
 21. Amale-sterile corn plant produced by the method of claim
 20. 22. A methodof producing an herbicide resistant corn plant comprising transformingthe corn plant of claim 4 with a transgene that confers herbicideresistance.
 23. An herbicide resistant corn plant produced by the methodof claim
 22. 24. A method of producing an insect resistant corn plantcomprising transforming the corn plant of claim 4 with a transgene thatconfers insect resistance.
 25. An insect resistant corn plant producedby the method of claim
 24. 26. A method of producing a disease resistantcorn plant comprising transforming the corn plant of claim 4 with atransgene that confers resistance to bacterial, fungal or viral disease.27. A disease resistant corn plant produced by the method of claim 26.28. A method of producing a corn plant with modified carbohydratecomposition comprising transforming the corn plant of claim 4 with atransgene conferring waxy starch.
 29. A corn plant produced by themethod of claim 28.